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dc.contributor.authorAho, Vesa
dc.contributor.authorMäntylä, Elina
dc.contributor.authorEkman, Axel
dc.contributor.authorHakanen, Satu
dc.contributor.authorMattola, Salla
dc.contributor.authorChen, Jian-Hua
dc.contributor.authorWeinhardt, Venera
dc.contributor.authorRuokolainen, Visa
dc.contributor.authorSodeik, Beate
dc.contributor.authorLarabell, Carolyn
dc.contributor.authorVihinen-Ranta, Maija
dc.date.accessioned2019-10-21T04:27:10Z
dc.date.available2019-10-21T04:27:10Z
dc.date.issued2019
dc.identifier.citationAho, Vesa; Mäntylä, Elina; Ekman, Axel; Hakanen, Satu; Mattola, Salla; Chen, Jian-Hua; Weinhardt, Venera; Ruokolainen, Visa; Sodeik, Beate; Larabell, Carolyn; Vihinen-Ranta, Maija (2019). Quantitative Microscopy Reveals Stepwise Alteration of Chromatin Structure during Herpesvirus Infection. Viruses, 11 (10), 935. DOI: 10.3390/v11100935
dc.identifier.otherCONVID_33277986
dc.identifier.urihttps://jyx.jyu.fi/handle/123456789/65964
dc.description.abstractDuring lytic herpes simplex virus 1 (HSV-1) infection, the expansion of the viral replication compartments leads to an enrichment of the host chromatin in the peripheral nucleoplasm. We have shown previously that HSV-1 infection induces the formation of channels through the compacted peripheral chromatin. Here, we used three-dimensional confocal and expansion microscopy, soft X-ray tomography, electron microscopy, and random walk simulations to analyze the kinetics of host chromatin redistribution and capsid localization relative to their egress site at the nuclear envelope. Our data demonstrated a gradual increase in chromatin marginalization, and the kinetics of chromatin smoothening around the viral replication compartments correlated with their expansion. We also observed a gradual transfer of capsids to the nuclear envelope. Later in the infection, random walk modeling indicated a gradually faster transport of capsids to the nuclear envelope that correlated with an increase in the interchromatin channels in the nuclear periphery. Our study reveals a stepwise and time-dependent mechanism of herpesvirus nuclear egress, in which progeny viral capsids approach the egress sites at the nuclear envelope via interchromatin spaces.en
dc.format.mimetypeapplication/pdf
dc.languageeng
dc.publisherMDPI
dc.relation.ispartofseriesViruses
dc.rightsCC BY 4.0
dc.subject.otherHSV-1
dc.subject.otherchromatin
dc.subject.othernuclear egress
dc.titleQuantitative Microscopy Reveals Stepwise Alteration of Chromatin Structure during Herpesvirus Infection
dc.typearticle
dc.identifier.urnURN:NBN:fi:jyu-201910214530
dc.contributor.laitosBio- ja ympäristötieteiden laitosfi
dc.contributor.laitosDepartment of Biological and Environmental Scienceen
dc.type.urihttp://purl.org/eprint/type/JournalArticle
dc.description.reviewstatuspeerReviewed
dc.relation.issn1999-4915
dc.relation.numberinseries10
dc.relation.volume11
dc.type.versionpublishedVersion
dc.rights.copyright© Authors, 2019
dc.rights.accesslevelopenAccessfi
dc.relation.grantnumber
dc.subject.ysotuma
dc.subject.ysoinfektiot
dc.subject.ysomikroskopia
dc.subject.ysoherpes simplex -virus
dc.subject.ysoherpesvirukset
dc.format.contentfulltext
jyx.subject.urihttp://www.yso.fi/onto/yso/p2411
jyx.subject.urihttp://www.yso.fi/onto/yso/p7316
jyx.subject.urihttp://www.yso.fi/onto/yso/p16290
jyx.subject.urihttp://www.yso.fi/onto/yso/p7738
jyx.subject.urihttp://www.yso.fi/onto/yso/p21634
dc.rights.urlhttps://creativecommons.org/licenses/by/4.0/
dc.relation.doi10.3390/v11100935
dc.relation.funderJane and Aatos Erkko Foundation
jyx.fundingprogramFoundation
jyx.fundinginformationThis research was funded by the Jane and Aatos Erkko Foundation (M.V-R.), the National Institute of General Medical Sciences of the National Institute of Health under the award number P41 GM103445, and the US Department of Energy, Biological and Environmental Research (DE-AC02-05CH11231, C.A.L.), and the German Research Foundation (CRC 900, project C2, B.S.)


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