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dc.contributor.authorLeppänen, Miika
dc.contributor.authorMaasilta, Ilari
dc.contributor.authorSundberg, Lotta-Riina
dc.date.accessioned2019-10-14T10:23:10Z
dc.date.available2019-10-14T10:23:10Z
dc.date.issued2019
dc.identifier.citationLeppänen, M., Maasilta, I., & Sundberg, L.-R. (2019). Antibacterial efficiency of surface-immobilized Flavobacterium-infecting bacteriophage. <i>ACS Applied Bio Materials</i>, <i>2</i>(11), 4720-4727. <a href="https://doi.org/10.1021/acsabm.9b00242" target="_blank">https://doi.org/10.1021/acsabm.9b00242</a>
dc.identifier.otherCONVID_33190463
dc.identifier.urihttps://jyx.jyu.fi/handle/123456789/65870
dc.description.abstractControl of bacterial diseases by bacteriophages (phages) is gaining more interest due to increasing antibiotic resistance. This has led to technologies to attach phages on surfaces to form a biomaterial that can functionally display phages that interact with bacteria, to carry out successful infection cycles. Such a material could be applied in many environments, where the target pathogens are expected. Although this approach has been applied successfully in a few studies already, the basis of the antibacterial effect by the immobilized phages is unclear, and the interpretation of the results depends on the study. Here, we studied the phage attachment density, their detachment rate and infectivity on five different surfaces: silicon, amine-treated silicon, gold, carboxylate-treated gold and crosslinker-activated carboxylate-treated gold. The density of attached phages varied between the different surfaces and was highest on the crosslinker-activated carboxylate-treated gold. To understand whether the antibacterial effect is caused by the attached or the detached phages, the strength of the immobilization was analyzed by performing 3-12 washing steps. The detachment rates differed between the materials, with the amine treated silicon surface generating the highest release of phages and maintaining the highest infectivity, even after extensive washing. On the other hand, covalent crosslinking seemed to interfere with the infectivity. Our results suggest that the detachment of the phages from the surface is a possible mechanism for the antibacterial effect. Furthermore, we introduce a measure of the infectivity by comparing the bacterial growth reductions produced by the phage-treated materials to the effect caused by a known number of free phages, resulting in a unit “Effective PFU/surface area”, a comparable standard between different studies.en
dc.format.mimetypeapplication/pdf
dc.languageeng
dc.language.isoeng
dc.publisherAmerican Chemical Society
dc.relation.ispartofseriesACS Applied Bio Materials
dc.rightsIn Copyright
dc.subject.otherphage therapy
dc.subject.othersurface adsorbed bacteriophages
dc.subject.otherantibacterial surfaces
dc.subject.otheraquaculture
dc.subject.othervirus material
dc.subject.otherbiomaterial
dc.titleAntibacterial efficiency of surface-immobilized Flavobacterium-infecting bacteriophage
dc.typearticle
dc.identifier.urnURN:NBN:fi:jyu-201910144450
dc.contributor.laitosBio- ja ympäristötieteiden laitosfi
dc.contributor.laitosFysiikan laitosfi
dc.contributor.laitosDepartment of Biological and Environmental Scienceen
dc.contributor.laitosDepartment of Physicsen
dc.contributor.oppiaineSolu- ja molekyylibiologiafi
dc.contributor.oppiaineNanoscience Centerfi
dc.contributor.oppiaineCell and Molecular Biologyen
dc.contributor.oppiaineNanoscience Centeren
dc.type.urihttp://purl.org/eprint/type/JournalArticle
dc.type.coarhttp://purl.org/coar/resource_type/c_2df8fbb1
dc.description.reviewstatuspeerReviewed
dc.format.pagerange4720-4727
dc.relation.issn2576-6422
dc.relation.numberinseries11
dc.relation.volume2
dc.type.versionacceptedVersion
dc.rights.copyright© 2019 American Chemical Society
dc.rights.accesslevelopenAccessfi
dc.relation.grantnumber
dc.relation.grantnumber266879
dc.relation.grantnumber314939
dc.subject.ysopinnat
dc.subject.ysobiomateriaalit
dc.subject.ysovesiviljely (kalatalous)
dc.subject.ysobakteriofagit
dc.subject.ysobakteeritaudit
dc.subject.ysofagiterapia
dc.format.contentfulltext
jyx.subject.urihttp://www.yso.fi/onto/yso/p20972
jyx.subject.urihttp://www.yso.fi/onto/yso/p711
jyx.subject.urihttp://www.yso.fi/onto/yso/p5099
jyx.subject.urihttp://www.yso.fi/onto/yso/p25303
jyx.subject.urihttp://www.yso.fi/onto/yso/p19293
jyx.subject.urihttp://www.yso.fi/onto/yso/p29496
dc.rights.urlhttp://rightsstatements.org/page/InC/1.0/?language=en
dc.relation.doi10.1021/acsabm.9b00242
dc.relation.funderJane ja Aatos Erkon säätiöfi
dc.relation.funderSuomen Akatemiafi
dc.relation.funderSuomen Akatemiafi
dc.relation.funderJane and Aatos Erkko Foundationen
dc.relation.funderResearch Council of Finlanden
dc.relation.funderResearch Council of Finlanden
jyx.fundingprogramSäätiöfi
jyx.fundingprogramAkatemiatutkija, SAfi
jyx.fundingprogramAkatemiahanke, SAfi
jyx.fundingprogramFoundationen
jyx.fundingprogramAcademy Research Fellow, AoFen
jyx.fundingprogramAcademy Project, AoFen
jyx.fundinginformationThe authors would like to thank Dr. Elina Laanto for providing the bacterial and phage isolate used in this study. L.-R. S. acknowledges funding from the Academy of Finland (grants #266879 and #314939), and from the Jane and Aatos Erkko Foundation.
dc.type.okmA1


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