Näytä suppeat kuvailutiedot

dc.contributor.authorMikonranta, Lauri
dc.contributor.authorBuckling, Angus
dc.contributor.authorJalasvuori, Matti
dc.contributor.authorRaymond, Ben
dc.date.accessioned2019-05-14T08:00:40Z
dc.date.available2019-05-14T08:00:40Z
dc.date.issued2019
dc.identifier.citationMikonranta, L., Buckling, A., Jalasvuori, M., & Raymond, B. (2019). Targeting antibiotic resistant bacteria with phage reduces bacterial density in an insect host. <i>Biology Letters</i>, <i>15</i>(3), Article 20180895. <a href="https://doi.org/10.1098/rsbl.2018.0895" target="_blank">https://doi.org/10.1098/rsbl.2018.0895</a>
dc.identifier.otherCONVID_28968117
dc.identifier.otherTUTKAID_80944
dc.identifier.urihttps://jyx.jyu.fi/handle/123456789/63920
dc.description.abstractPhage therapy is attracting growing interest among clinicians as antibiotic resistance continues becoming harder to control. However, clinical trials and animal model studies on bacteriophage treatment are still scarce and results on the efficacy vary. Recent research suggests that using traditional antimicrobials in concert with phage could have desirable synergistic effects that hinder the evolution of resistance. Here, we present a novel insect gut model to study phage–antibiotic interaction in a system where antibiotic resistance initially exists in very low frequency and phage specifically targets the resistance bearing cells. We demonstrate that while phage therapy could not reduce the frequency of target bacteria in the population during positive selection by antibiotics, it alleviated the antibiotic induced blooming by lowering the overall load of resistant cells. The highly structured gut environment had pharmacokinetic effects on both phage and antibiotic dynamics compared with in vitro: antibiotics did not reduce the overall amount of bacteria, demonstrating a simple turnover of gut microbiota from non-resistant to resistant population with little cost. The results imply moderate potential for using phage as an aid to target antibiotic resistant gut infections, and question the usefulness of in vitro inferences.en
dc.format.mimetypeapplication/pdf
dc.language.isoeng
dc.publisherRoyal Society Publishing
dc.relation.ispartofseriesBiology Letters
dc.rightsIn Copyright
dc.subject.otherbacteriophage
dc.subject.otherEnterobacter cloacae
dc.subject.othergut infection
dc.subject.otherinsect model
dc.titleTargeting antibiotic resistant bacteria with phage reduces bacterial density in an insect host
dc.typearticle
dc.identifier.urnURN:NBN:fi:jyu-201905102532
dc.contributor.laitosBio- ja ympäristötieteiden laitosfi
dc.contributor.laitosDepartment of Biological and Environmental Scienceen
dc.contributor.oppiaineSolu- ja molekyylibiologiafi
dc.contributor.oppiaineNanoscience Centerfi
dc.contributor.oppiaineCell and Molecular Biologyen
dc.contributor.oppiaineNanoscience Centeren
dc.type.urihttp://purl.org/eprint/type/JournalArticle
dc.date.updated2019-05-10T09:15:06Z
dc.type.coarhttp://purl.org/coar/resource_type/c_2df8fbb1
dc.description.reviewstatuspeerReviewed
dc.relation.issn1744-9561
dc.relation.numberinseries3
dc.relation.volume15
dc.type.versionacceptedVersion
dc.rights.copyright© 2019 The Author(s)
dc.rights.accesslevelopenAccessfi
dc.relation.grantnumber297049
dc.subject.ysoantibioottiresistenssi
dc.subject.ysoenterobakteerit
dc.subject.ysobakteriofagit
dc.subject.ysofagiterapia
dc.format.contentfulltext
jyx.subject.urihttp://www.yso.fi/onto/yso/p29640
jyx.subject.urihttp://www.yso.fi/onto/yso/p28554
jyx.subject.urihttp://www.yso.fi/onto/yso/p25303
jyx.subject.urihttp://www.yso.fi/onto/yso/p29496
dc.rights.urlhttp://rightsstatements.org/page/InC/1.0/?language=en
dc.relation.datasethttp://dx.doi.org/10.5061/dryad.sc54383
dc.relation.doi10.1098/rsbl.2018.0895
dc.relation.funderSuomen Akatemiafi
dc.relation.funderAcademy of Finlanden
jyx.fundingprogramAkatemiatutkija, SAfi
jyx.fundingprogramAcademy Research Fellow, AoFen
jyx.fundinginformationThe work was funded by an MRC innovation award (MR/N013824/1), Academy of Finland grants (252411 and 297049) and Emil Aaltonen Foundation grant.
dc.type.okmA1


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