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dc.contributor.authorPuttaraksa, Kanoktip
dc.contributor.authorPirttinen, Heidi
dc.contributor.authorKarvonen, Kati
dc.contributor.authorNykky, Jonna
dc.contributor.authorNaides, Stanley J.
dc.contributor.authorGilbert, Leona
dc.date.accessioned2019-04-23T09:03:21Z
dc.date.available2019-04-23T09:03:21Z
dc.date.issued2019fi
dc.identifier.citationPuttaraksa, K., Pirttinen, H., Karvonen, K., Nykky, J., Naides, S. J., & Gilbert, L. (2019). Parvovirus B19V Non-structural Protein NS1 Induces dsDNA Autoantibodies and End Organ Damage in Non-autoimmune Mice. <em>Journal of Infectious Diseases</em>, 219 (9), 1418-1429. <a href="https://doi.org/10.1093/infdis/jiy614">doi:10.1093/infdis/jiy614</a>fi
dc.identifier.otherTUTKAID_79284
dc.identifier.urihttps://jyx.jyu.fi/handle/123456789/63573
dc.description.abstractBackground Viral infection is implicated in development of autoimmunity. Parvovirus B19 (B19V) nonstructural protein, NS1, a helicase, covalently modifies self double-stranded deoxyribonucleic acid (dsDNA) and induces apoptosis. This study tested whether resulting apoptotic bodies (ApoBods) containing virally modified dsDNA could induce autoimmunity in an animal model. Methods BALB/c mice were inoculated with (1) pristane-induced, (2) B19V NS1-induced, or (3) staurosporine-induced ApoBods. Serum was tested for dsDNA autoantibodies by Crithidia luciliae staining and enzyme-linked immunosorbent assay. Brain, heart, liver, and kidney pathology was examined. Deposition of self-antigens in glomeruli was examined by staining with antibodies to dsDNA, histones H1 and H4, and TATA-binding protein. Results The B19V NS1-induced ApoBod inoculation induced dsDNA autoantibodies in a dose-dependent fashion. Histopathological features of immune-mediated organ damage were evident in pristane-induced and NS1-induced ApoBod groups; severity scores were higher in these groups than in staurosporine-treated groups. Tissue damage was dependent on NS1-induced ApoBod dose. Nucleosomal antigens were deposited in target tissue from pristane-induced and NS1-induced ApoBod inoculated groups, but not in the staurosporine-induced ApoBod inoculated group. Conclusions This study demonstrated proof of principle in an animal model that virally modified dsDNA in apoptotic bodies could break tolerance to self dsDNA and induce dsDNA autoantibodies and end-organ damage.fi
dc.format.mimetypeapplication/pdf
dc.language.isoeng
dc.publisherOxford University Press
dc.relation.ispartofseriesJournal of Infectious Diseases
dc.rightsCC BY-NC-ND 4.0
dc.subject.otherviruksetfi
dc.subject.otherparvoviruksetfi
dc.subject.otherinfektiotfi
dc.subject.othervirustauditfi
dc.subject.otherautoimmuniteettifi
dc.subject.otherautovasta-aineetfi
dc.subject.otherohjelmoitunut solukuolemafi
dc.subject.otherparvovirusfi
dc.subject.otherB19Vfi
dc.subject.otherapoptosisfi
dc.subject.otherapoptotic bodiesfi
dc.subject.otheranti-dsDNA antibodyfi
dc.subject.otherSLEfi
dc.subject.otherglomerulonephritisfi
dc.subject.otherhepatitisfi
dc.subject.othertolerancefi
dc.titleParvovirus B19V Non-structural Protein NS1 Induces dsDNA Autoantibodies and End Organ Damage in Non-autoimmune Micefi
dc.typearticle
dc.identifier.urnURN:NBN:fi:jyu-201904182227
dc.contributor.laitosBio- ja ympäristötieteiden laitosfi
dc.contributor.laitosThe Department of Biological and Environmental Scienceen
dc.contributor.oppiaineSolu- ja molekyylibiologia
dc.type.urihttp://purl.org/eprint/type/JournalArticle
dc.date.updated2019-04-18T12:15:09Z
dc.description.reviewstatuspeerReviewed
dc.format.pagerange1418-1429
dc.relation.issn0022-1899
dc.relation.numberinseries9
dc.relation.volume219
dc.type.versionpublishedVersion
dc.rights.copyright© The Author(s) 2018.
dc.rights.accesslevelopenAccessfi
dc.format.contentfulltext
dc.rights.urlhttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.relation.doi10.1093/infdis/jiy614


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