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dc.contributor.authorVinner, Gurinder K.
dc.contributor.authorRezaie-Yazdi, Zahra
dc.contributor.authorLeppänen, Miika
dc.contributor.authorStapley, Andrew G.F.
dc.contributor.authorLeaper, Mark C.
dc.contributor.authorMalik, Danish J.
dc.date.accessioned2019-04-08T10:12:04Z
dc.date.available2019-04-08T10:12:04Z
dc.date.issued2019
dc.identifier.citationVinner, G. K., Rezaie-Yazdi, Z., Leppänen, M., Stapley, A. G., Leaper, M. C., & Malik, D. J. (2019). Microencapsulation of Salmonella-Specific Bacteriophage Felix O1 Using Spray-Drying in a pH-Responsive Formulation and Direct Compression Tableting of Powders into a Solid Oral Dosage Form. <i>Pharmaceuticals</i>, <i>12</i>(1), Article 43. <a href="https://doi.org/10.3390/ph12010043" target="_blank">https://doi.org/10.3390/ph12010043</a>
dc.identifier.otherCONVID_28992352
dc.identifier.otherTUTKAID_81079
dc.identifier.urihttps://jyx.jyu.fi/handle/123456789/63430
dc.description.abstractThe treatment of enteric bacterial infections using oral bacteriophage therapy can be challenging since the harsh acidic stomach environment renders phages inactive during transit through the gastrointestinal tract. Solid oral dosage forms allowing site-specific gastrointestinal delivery of high doses of phages, e.g., using a pH or enzymatic trigger, would be a game changer for the nascent industry trying to demonstrate the efficacy of phages, including engineered phages for gut microbiome modulation in expensive clinical trials. Spray-drying is a scalable, low-cost process for producing pharmaceutical agents in dry powder form. Encapsulation of a model Salmonella-specific phage (Myoviridae phage Felix O1) was carried out using the process of spray-drying, employing a commercially available Eudragit S100® pH-responsive anionic copolymer composed of methyl methacrylate-co-methacrylic acid formulated with trehalose. Formulation and processing conditions were optimised to improve the survival of phages during spray-drying, and their subsequent protection upon exposure to simulated gastric acidity was demonstrated. Addition of trehalose to the formulation was shown to protect phages from elevated temperatures and desiccation encountered during spray-drying. Direct compression of spray-dried encapsulated phages into tablets was shown to significantly improve phage protection upon exposure to simulated gastric fluid. The results reported here demonstrate the significant potential of spray-dried pH-responsive formulations for oral delivery of bacteriophages targeting gastrointestinal applications.fi
dc.format.mimetypeapplication/pdf
dc.language.isoeng
dc.publisherMDPI AG
dc.relation.ispartofseriesPharmaceuticals
dc.rightsCC BY 4.0
dc.subject.otherdirect compression
dc.subject.othermicroparticles
dc.subject.otherpH-responsive
dc.subject.otherspray drying
dc.subject.othersalmonella
dc.subject.othertablets
dc.titleMicroencapsulation of Salmonella-Specific Bacteriophage Felix O1 Using Spray-Drying in a pH-Responsive Formulation and Direct Compression Tableting of Powders into a Solid Oral Dosage Form
dc.typearticle
dc.identifier.urnURN:NBN:fi:jyu-201903292012
dc.contributor.laitosBio- ja ympäristötieteiden laitosfi
dc.contributor.laitosDepartment of Biological and Environmental Scienceen
dc.contributor.oppiaineSolu- ja molekyylibiologiafi
dc.contributor.oppiaineNanoscience Centerfi
dc.contributor.oppiaineCell and Molecular Biologyen
dc.contributor.oppiaineNanoscience Centeren
dc.type.urihttp://purl.org/eprint/type/JournalArticle
dc.date.updated2019-03-29T16:15:11Z
dc.type.coarhttp://purl.org/coar/resource_type/c_2df8fbb1
dc.description.reviewstatuspeerReviewed
dc.relation.issn1424-8247
dc.relation.numberinseries1
dc.relation.volume12
dc.type.versionpublishedVersion
dc.rights.copyright© 2019 by The Authors.
dc.rights.accesslevelopenAccessfi
dc.subject.ysoantibioottiresistenssi
dc.subject.ysolääkesuunnittelu
dc.subject.ysotabletit (puristeet)
dc.subject.ysofagiterapia
dc.subject.ysobakteriofagit
dc.subject.ysoSalmonella-bakteerit
dc.format.contentfulltext
jyx.subject.urihttp://www.yso.fi/onto/yso/p29640
jyx.subject.urihttp://www.yso.fi/onto/yso/p25180
jyx.subject.urihttp://www.yso.fi/onto/yso/p23221
jyx.subject.urihttp://www.yso.fi/onto/yso/p29496
jyx.subject.urihttp://www.yso.fi/onto/yso/p25303
jyx.subject.urihttp://www.yso.fi/onto/yso/p15496
dc.rights.urlhttps://creativecommons.org/licenses/by/4.0/
dc.relation.doi10.3390/ph12010043
dc.type.okmA1


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