Viral highway to nucleus exposed by image correlation analyses
Mäntylä, E., Chacko, J. V., Aho, V., Parrish, C. R., Shahin, V., Kann, M., Digman, M. A., Gratton, E., & Vihinen-Ranta, M. (2018). Viral highway to nucleus exposed by image correlation analyses. Scientific Reports, 8, Article 1152. https://doi.org/10.1038/s41598-018-19582-w
Published inScientific Reports
© Te Author(s) 2018. This is an open access article distributed under the terms of the Creative Commons License.
Parvoviral genome translocation from the plasma membrane into the nucleus is a coordinated multistep process mediated by capsid proteins. We used fast confocal microscopy line scan imaging combined with image correlation methods including auto-, pair- and cross-correlation, and number and brightness analysis, to study the parvovirus entry pathway at the single-particle level in living cells. Our results show that the endosome-associated movement of virus particles fluctuates from fast to slow. Fast transit of single cytoplasmic capsids to the nuclear envelope is followed by slow movement of capsids and fast diffusion of capsid fragments in the nucleoplasm. The unique combination of image analyses allowed us to follow the fate of intracellular single virus particles and their interactions with importin β revealing previously unknown dynamics of the entry pathway.
PublisherNature Publishing Group
ISSN Search the Publication Forum2045-2322
Publication in research information system
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Except where otherwise noted, this item's license is described as © Te Author(s) 2018. This is an open access article distributed under the terms of the Creative Commons License.
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