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dc.contributor.authorMustonen, Marina
dc.contributor.authorHaimi, Jari
dc.contributor.authorKesäniemi, Jenni
dc.contributor.authorHögmander, Harri
dc.contributor.authorKnott, Emily
dc.date.accessioned2017-04-12T09:28:54Z
dc.date.available2017-04-12T09:28:54Z
dc.date.issued2017
dc.identifier.citationMustonen, M., Haimi, J., Kesäniemi, J., Högmander, H., & Knott, E. (2017). Variation in gene expression within clones of the earthworm Dendrobaena octaedra. <em>PLoS ONE</em>, 12 (4), e0174960. <a href="https://doi.org/10.1371/journal.pone.0174960">doi:10.1371/journal.pone.0174960</a>
dc.identifier.otherTUTKAID_73485
dc.identifier.urihttps://jyx.jyu.fi/handle/123456789/53585
dc.description.abstractGene expression is highly plastic, which can help organisms to both acclimate and adapt to changing environments. Possible variation in gene expression among individuals with the same genotype (among clones) is not widely considered, even though it could impact the results of studies that focus on gene expression phenotypes, for example studies using clonal lines. We examined the extent of within and between clone variation in gene expression in the earthworm Dendrobaena octaedra, which reproduces through apomictic parthenogenesis. Five microsatellite markers were developed and used to confirm that offspring are genetic clones of their parent. After that, expression of 12 genes was measured from five individuals each from six clonal lines after exposure to copper contaminated soil. Variation in gene expression was higher over all genotypes than within genotypes, as initially assumed. A subset of the genes was also examined in the offspring of exposed individuals in two of the clonal lines. In this case, variation in gene expression within genotypes was as high as that observed over all genotypes. One gene in particular (chymotrypsin inhibitor) also showed significant differences in the expression levels among genetically identical individuals. Gene expression can vary considerably, and the extent of variation may depend on the genotypes and genes studied. Ensuring a large sample, with many different genotypes, is critical in studies comparing gene expression phenotypes. Researchers should be especially cautious inferring gene expression phenotypes when using only a single clonal or inbred line, since the results might be specific to only certain genotypes.
dc.language.isoeng
dc.publisherPublic Library of Science
dc.relation.ispartofseriesPLoS ONE
dc.subject.othergene expression
dc.subject.othergenotype
dc.subject.otherclones
dc.subject.otherparthenogenesis
dc.subject.otherearthworms
dc.subject.otherDendrobaena octaedra
dc.titleVariation in gene expression within clones of the earthworm Dendrobaena octaedra
dc.typearticle
dc.identifier.urnURN:NBN:fi:jyu-201704121977
dc.contributor.laitosBio- ja ympäristötieteiden laitosfi
dc.contributor.laitosMatematiikan ja tilastotieteen laitosfi
dc.contributor.laitosThe Department of Biological and Environmental Scienceen
dc.contributor.laitosDepartment of Mathematics and Statisticsen
dc.contributor.oppiaineEkologia ja evoluutiobiologia
dc.contributor.oppiaineTilastotiede
dc.type.urihttp://purl.org/eprint/type/JournalArticle
dc.date.updated2017-04-12T09:15:07Z
dc.type.coarjournal article
dc.description.reviewstatuspeerReviewed
dc.relation.issn1932-6203
dc.relation.volume12
dc.type.versionpublishedVersion
dc.rights.copyright© 2017 Mustonen et al. This is an open access article distributed under the terms of the Creative Commons Attribution License.
dc.rights.accesslevelopenAccessfi
dc.rights.urlhttps://creativecommons.org/licenses/by/4.0/
dc.relation.doi10.1371/journal.pone.0174960


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© 2017 Mustonen et al. This is an open access article distributed under the terms of the
Creative Commons Attribution License.
Except where otherwise noted, this item's license is described as © 2017 Mustonen et al. This is an open access article distributed under the terms of the Creative Commons Attribution License.