Variation in gene expression within clones of the earthworm Dendrobaena octaedra
Mustonen, M., Haimi, J., Kesäniemi, J., Högmander, H., & Knott, E. (2017). Variation in gene expression within clones of the earthworm Dendrobaena octaedra. PLoS ONE, 12(4), Article e0174960. https://doi.org/10.1371/journal.pone.0174960
Published inPLoS ONE
© 2017 Mustonen et al. This is an open access article distributed under the terms of the Creative Commons Attribution License.
Gene expression is highly plastic, which can help organisms to both acclimate and adapt to changing environments. Possible variation in gene expression among individuals with the same genotype (among clones) is not widely considered, even though it could impact the results of studies that focus on gene expression phenotypes, for example studies using clonal lines. We examined the extent of within and between clone variation in gene expression in the earthworm Dendrobaena octaedra, which reproduces through apomictic parthenogenesis. Five microsatellite markers were developed and used to confirm that offspring are genetic clones of their parent. After that, expression of 12 genes was measured from five individuals each from six clonal lines after exposure to copper contaminated soil. Variation in gene expression was higher over all genotypes than within genotypes, as initially assumed. A subset of the genes was also examined in the offspring of exposed individuals in two of the clonal lines. In this case, variation in gene expression within genotypes was as high as that observed over all genotypes. One gene in particular (chymotrypsin inhibitor) also showed significant differences in the expression levels among genetically identical individuals. Gene expression can vary considerably, and the extent of variation may depend on the genotypes and genes studied. Ensuring a large sample, with many different genotypes, is critical in studies comparing gene expression phenotypes. Researchers should be especially cautious inferring gene expression phenotypes when using only a single clonal or inbred line, since the results might be specific to only certain genotypes. ...
PublisherPublic Library of Science
ISSN Search the Publication Forum1932-6203
Publication in research information system
MetadataShow full item record
Except where otherwise noted, this item's license is described as © 2017 Mustonen et al. This is an open access article distributed under the terms of the Creative Commons Attribution License.
Showing items with similar title or keywords.
Genetic diversity of a clonal earthworm : gene expression variation and impacts on decomposition in metal contaminated soil Mustonen, Marina (University of Jyväskylä, 2016)Biodiversity affects ecosystem functioning positively. Metal-contamination diminishes species diversity and ecosystem function depends on the remaining species, and the genotypes within them. I studied how the clonally ...
Variants associated with HHIP expression have sex-differential effects on lung function [version 1; peer review: 2 approved] Fawcett, KA; Obeidat, M; Melbourne, C; Shrine, N; Guyatt, AL; John, C; Luan, J; Richmond, A; Moksnes, MR; Granell, R; Weiss, S; Imboden, M; May-Wilson, S; Hysi, P; Boutin, TS; Portas, L; Flexeder, C; Harris, SE; Wang, CA; Lyytikäinen, LP; Palviainen, T; Foong, RE; Keidel, D; Minelli, C; Langenberg, C; Bossé, Y; Van den Berge, M; Sin, DD; Hao, K; Campbell, A; Porteous, D; Padmanabhan, S; Smith, BH; Evans, DM; Ring, S; Langhammer, A; Hveem, K; Willer, C; Ewert, R; Stubbe, B; Pirastu, N; Klaric, L; Joshi, PK; Patasova, K; Massimo, M; Polasek, O; Starr, JM; Karrasch, S; Strauch, K; Meitinger, T; Rudan, I; Rantanen, T.; Pietiläinen, K; Kähönen, M; Raitakari, OT; Hall, GL; Sly, PD; Pennell, CE; Kaprio, J; Lehtimäki, T; Vitart, V; Deary, IJ; Jarvis, D; Wilson, JF; Spector, T; Probst-Hensch, N; Wareham, NJ; Völzke, H; Henderson, J; Strachan, DP; Brumpton, BM; Hayward, C; Hall, IP; Tobin, MD; Wain, LV (Wellcome Trust, 2020)Background: Lung function is highly heritable and differs between the sexes throughout life. However, little is known about sex-differential genetic effects on lung function. We aimed to conduct the first genome-wide ...
Hormone Replacement Therapy Associated White Blood Cell DNA Methylation and Gene Expression are Associated With Within-Pair Differences of Body Adiposity and Bone Mass Bahl, Aileen; Pöllänen, Eija; Ismail, Khadeeja; Sipilä, Sarianna; Mikkola, Tuija; Berglund, Eva; Lindqvist, Carl Mårten; Syvänen, Ann-Christine; Rantanen, Taina; Kaprio, Jaakko; Kovanen, Vuokko; Ollikainen, Miina (Cambridge University Press; International Society for Twin Studies, 2015)The loss of estrogen during menopause causes changes in the female body, with wide-ranging effects on health. Estrogen-containing hormone replacement therapy (HRT) leads to a relief of typical menopausal symptoms, benefits ...