Soft X-Ray Tomography Reveals Gradual Chromatin Compaction and Reorganization during Neurogenesis In Vivo
Gros, M. A. L., Clowney, E. J., Magklara, A., Yen, A., Markenscoff-Papadimitriou, E., Colquitt, B., Myllys, M., Kellis, M., Lomvardas, S., & Larabell, C. A. (2016). Soft X-Ray Tomography Reveals Gradual Chromatin Compaction and Reorganization during Neurogenesis In Vivo. Cell Reports, 17(8), 2125-2136. https://doi.org/10.1016/j.celrep.2016.10.060
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Cell ReportsAuthors
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2016Copyright
© 2016 The Author(s). This is an open access article under the CC BY-NC-ND license.
The realization that nuclear distribution of DNA, RNA,
and proteins differs between cell types and developmental
stages suggests that nuclear organization
serves regulatory functions. Understanding the logic
of nuclear architecture and how it contributes to differentiation
and cell fate commitment remains challenging.
Here, we use soft X-ray tomography (SXT)
to image chromatin organization, distribution, and
biophysical properties during neurogenesis in vivo.
Our analyses reveal that chromatin with similar biophysical
properties forms an elaborate connected
network throughout the entire nucleus. Although
this interconnectivity is present in every developmental
stage, differentiation proceeds with concomitant
increase in chromatin compaction and re-distribution
of condensed chromatin toward the nuclear
core. HP1b, but not nucleosome spacing or phasing,
regulates chromatin rearrangements because it governs
both the compaction of chromatin and its interactions
with the nuclear envelope. Our experiments
introduce SXT as a powerful imaging technology for
nuclear architecture.
...


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ElsevierISSN Search the Publication Forum
2211-1247Keywords
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Except where otherwise noted, this item's license is described as © 2016 The Author(s). This is an open access article under the CC BY-NC-ND license.
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