Näytä suppeat kuvailutiedot

dc.contributor.authorVehniäinen, Eeva-Riikka
dc.contributor.authorKukkonen, Jussi
dc.date.accessioned2016-08-11T06:03:26Z
dc.date.available2016-12-27T22:45:06Z
dc.date.issued2015
dc.identifier.citationVehniäinen, E.-R., & Kukkonen, J. (2015). Multixenobiotic resistance efflux activity in Daphnia magna and Lumbriculus variegatus. <i>Chemosphere</i>, <i>124</i>(April 2015), 143-149. <a href="https://doi.org/10.1016/j.chemosphere.2014.12.014" target="_blank">https://doi.org/10.1016/j.chemosphere.2014.12.014</a>
dc.identifier.otherCONVID_24465672
dc.identifier.urihttps://jyx.jyu.fi/handle/123456789/50929
dc.description.abstractMultixenobiotic resistance is a phenomenon in which ATP-binding cassette (ABC) family proteins transfer harmful compounds out of cells. Daphnia magna and Lumbriculus variegatus are model species in aquatic ecotoxicology, but the presence and activity of ABC proteins have not been well described in these species. The aim of this work was to study the presence, activity, and inhibition of ABC transport proteins in D. magna and L. variegatus. The presence of abcb1 and abcc transcripts in 8–9-day-old D. magna was investigated by qRT-PCR. The activity of MXR in D. magna and L. variegatus was explored by influx of the fluorescent ABC protein substrates rhodamine B and calcein-AM, with and without the model inhibitors verapamil (unspecific ABC inhibitor), reversin 205 (ABCB1 inhibitor) and MK571 (ABCC inhibitor). Juvenile D. magna possessed all examined abcb and abcc transcripts, but only reversin 205 inhibited MXR activity. The MXR activity in L. variegatus was inhibited by MK571, and to a lesser extent by verapamil, whereas reversin 205 seemed to stimulate the transport activity. Whereas calcein-AM worked better as an MXR substrate in D. magna, rhodamine B was a better substrate for L. variegatus MXR activity measurements. This is the first report on MXR activity in the order Lumbriculida, subclass Oligochaeta, and class Clitellata.
dc.language.isoeng
dc.publisherPergamon
dc.relation.ispartofseriesChemosphere
dc.subject.otherABC transfer protein
dc.subject.otherDaphnia magna
dc.subject.otherLumbriculus variegatus
dc.subject.othermultixenobiotic resistance
dc.subject.otherMXR activity
dc.titleMultixenobiotic resistance efflux activity in Daphnia magna and Lumbriculus variegatus
dc.typeresearch article
dc.identifier.urnURN:NBN:fi:jyu-201608103763
dc.contributor.laitosBio- ja ympäristötieteiden laitosfi
dc.contributor.laitosDepartment of Biological and Environmental Scienceen
dc.contributor.oppiaineYmpäristötiedefi
dc.contributor.oppiaineEnvironmental Scienceen
dc.type.urihttp://purl.org/eprint/type/JournalArticle
dc.date.updated2016-08-10T15:15:14Z
dc.type.coarhttp://purl.org/coar/resource_type/c_2df8fbb1
dc.description.reviewstatuspeerReviewed
dc.format.pagerange143–149
dc.relation.issn0045-6535
dc.relation.numberinseriesApril 2015
dc.relation.volume124
dc.type.versionacceptedVersion
dc.rights.copyright© Elsevier. This is a final draft version of an article whose final and definitive version has been published by Elsevier.
dc.rights.accesslevelopenAccessfi
dc.type.publicationarticle
dc.relation.doi10.1016/j.chemosphere.2014.12.014
dc.type.okmA1


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