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dc.contributor.authorSE, Krieger
dc.contributor.authorC, Kim
dc.contributor.authorL, Zhang
dc.contributor.authorMarjomäki, Varpu
dc.contributor.authorJ, Bergelson
dc.date.accessioned2016-03-16T09:21:18Z
dc.date.available2016-03-16T09:21:18Z
dc.date.issued2013
dc.identifier.citationSE, K., C, K., L, Z., Marjomäki, V., & J, B. (2013). Echovirus 1 entry into polarized Caco-2 cells depends on dynamin, cholesterol, and cellular factors associated with macropinocytosis. <i>Journal of Virology</i>, <i>87</i>(16), 8884-8895. <a href="https://doi.org/10.1128/JVI.03415-12" target="_blank">https://doi.org/10.1128/JVI.03415-12</a>
dc.identifier.otherCONVID_22995772
dc.identifier.otherTUTKAID_59105
dc.identifier.urihttps://jyx.jyu.fi/handle/123456789/49074
dc.description.abstractEnteroviruses invade their hosts by crossing the intestinal epithelium. We have examined the mechanism by which echovirus 1 (EV1) enters polarized intestinal epithelial cells (Caco-2). Virus binds to VLA-2 on the apical cell surface and moves rapidly to early endosomes. Using inhibitory drugs, dominant negative mutants, and small interfering RNAs (siRNAs) to block specific endocytic pathways, we found that virus entry requires dynamin GTPase and membrane cholesterol but is independent of both clathrin- and caveolin-mediated endocytosis. Instead, infection requires factors commonly associated with macropinocytosis, including amiloride-sensitive Na /H exchange, protein kinase C, and C-terminal-binding protein-1 (CtBP1); furthermore, EV1 accumulates rapidly in intracellular vesicles with dextran, a fluid-phase marker. These results suggest a role for macropinocytosis in the process by which EV1 enters polarized cells to initiate infection.
dc.language.isoeng
dc.publisherAmerican Society for Microbiology
dc.relation.ispartofseriesJournal of Virology
dc.subject.otherechovirus
dc.subject.otherinfektio
dc.subject.otherendosomi
dc.subject.otherinfection
dc.subject.otherendosome
dc.titleEchovirus 1 entry into polarized Caco-2 cells depends on dynamin, cholesterol, and cellular factors associated with macropinocytosis
dc.typearticle
dc.identifier.urnURN:NBN:fi:jyu-201603101814
dc.contributor.laitosBio- ja ympäristötieteiden laitosfi
dc.contributor.laitosDepartment of Biological and Environmental Scienceen
dc.contributor.oppiaineSolu- ja molekyylibiologiafi
dc.contributor.oppiaineCell and Molecular Biologyen
dc.type.urihttp://purl.org/eprint/type/JournalArticle
dc.date.updated2016-03-10T10:15:43Z
dc.type.coarhttp://purl.org/coar/resource_type/c_2df8fbb1
dc.description.reviewstatuspeerReviewed
dc.format.pagerange8884-8895
dc.relation.issn1098-5514
dc.relation.numberinseries16
dc.relation.volume87
dc.type.versionpublishedVersion
dc.rights.copyright© 2013, American Society for Microbiology. Published in this repository with the kind permission of the publisher.
dc.rights.accesslevelopenAccessfi
dc.relation.doi10.1128/JVI.03415-12
dc.type.okmA1


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