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dc.contributor.advisorKainulainen, Heikki
dc.contributor.advisorFachada, Vasco
dc.contributor.authorTriantou, Vasiliki
dc.date.accessioned2015-12-02T15:46:09Z
dc.date.available2015-12-02T15:46:09Z
dc.date.issued2015
dc.identifier.otheroai:jykdok.linneanet.fi:1505173
dc.identifier.urihttps://jyx.jyu.fi/handle/123456789/47964
dc.description.abstractIn the modern world, diet patterns high on lipids, sedentary lifestyle and obesity contribute all in the development of metabolic syndrome, which in turn can lead to type 2 diabetes. All the excess fats, that the majority of the population consumes nowadays, are stored as ectopic fat, particularly in the skeletal muscle, a tissue beyond doubt insulin-sensitive. Physical activity has been proposed according to various studies to be the most drastic factor, which can induce changes in skeletal muscle lipid metabolism,as well as different diet models. We hypothesized that different exercise patterns and diet-changes can alter the spatial arrangement in lipid droplets (LDs) in different skeletal muscle fiber types of C57BL/6J mice. Muscle samples from gastrocnemius were collected from 8 groups: sedentary controls fed with a high-fat (HFD, n=10), or a low-fat diet (LFD, n=10), chronic exercise group fed with a high-fat (HFDR, n=10) or a low-fat diet (LFDR, n=10) and acute exercise group that either killed immediately after exercise or 6 hours later following a high-fat (HFD0, n=7 or HFDR6, n=7) or a low-fat diet (LFDR0, n=7 or LFDR6, n=7). Then immunohistochemistry and laser confocal microscopy were used, followed by image analysis. Fiber type LD size showed a significant difference between all groups, apart from the chronic exercise group fed with the low-fat diet and the acute exercise group fed with the high-fat diet and sacrificed 6 hours after exhaustion. Fiber type LD density showed a very significant difference(P<0.001) between all groups. It seems that physical activity with or without the highfat diet, induced changes in size and density of LDs, meaning that those two factors exercise and diet play a critical role in the prevention of metabolic syndrome later on. A precise comprehension of metabolic pathways participating in obesity and metabolic syndrome reinforces the worldwide effort of diminishing or totally eliminate them.en
dc.format.extent1 verkkoaineisto (71 sivua)
dc.language.isoeng
dc.rightsJulkaisu on tekijänoikeussäännösten alainen. Teosta voi lukea ja tulostaa henkilökohtaista käyttöä varten. Käyttö kaupallisiin tarkoituksiin on kielletty.fi
dc.rightsThis publication is copyrighted. You may download, display and print it for Your own personal use. Commercial use is prohibited.en
dc.subject.otherlipid metabolism
dc.subject.otherlipid droplets
dc.subject.otherphysical activity
dc.subject.otherinsulin resistance
dc.subject.otherobesity
dc.subject.othermice
dc.subject.otherdiet
dc.subject.otherfiber typing
dc.titleComparison of acute and chronic exercise effects in the lipid droplets topography skeletal muscle, following high and low-fat diet in mice
dc.identifier.urnURN:NBN:fi:jyu-201512023898
dc.type.ontasotPro gradufi
dc.type.ontasotMaster's thesisen
dc.contributor.tiedekuntaLiikuntatieteellinen tiedekuntafi
dc.contributor.tiedekuntaFaculty of Sport and Health Sciencesen
dc.contributor.laitosLiikuntabiologian laitosfi
dc.contributor.laitosDepartment of Biology of Physical Activityen
dc.contributor.yliopistoUniversity of Jyväskyläen
dc.contributor.yliopistoJyväskylän yliopistofi
dc.contributor.oppiaineLiikuntafysiologiafi
dc.contributor.oppiaineExercise Physiologyen
dc.date.updated2015-12-02T15:46:10Z
dc.rights.accesslevelopenAccessfi
dc.contributor.oppiainekoodi5011
dc.subject.ysoaineenvaihdunta
dc.subject.ysohiiret
dc.subject.ysoruokavaliot
dc.subject.ysofyysinen aktiivisuus
dc.subject.ysolihavuus
dc.subject.ysoinsuliiniresistenssi
dc.subject.ysorasvat


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