Näytä suppeat kuvailutiedot

dc.contributor.authorNykky, Jonna
dc.contributor.authorTuusa, Jenni
dc.contributor.authorKirjavainen, Sanna
dc.contributor.authorVuento, Matti
dc.contributor.authorGilbert, Leona
dc.date.accessioned2015-11-06T09:21:19Z
dc.date.available2015-11-06T09:21:19Z
dc.date.issued2010
dc.identifier.citationNykky, J., Tuusa, J., Kirjavainen, S., Vuento, M., & Gilbert, L. (2010). Mechanisms of cell death in canine parvovirus-infected cells provide intuitive insights to developing nanotools for medicine. <i>International Journal of Nanomedicine</i>, <i>5</i>, 417-428. <a href="https://doi.org/10.2147/ijn.s10579" target="_blank">https://doi.org/10.2147/ijn.s10579</a>
dc.identifier.otherCONVID_19584480
dc.identifier.otherTUTKAID_41069
dc.identifier.urihttps://jyx.jyu.fi/handle/123456789/47581
dc.description.abstractViruses have great potential as nanotools in medicine for gene transfer, targeted gene delivery, and oncolytic cancer virotherapy. Here we have studied cell death mechanisms of canine parvovirus (CPV) to increase the knowledge on the CPV life cycle in order to facilitate the development of better parvovirus vectors. Morphological studies of CPV-infected Norden laboratory feline kidney (NLFK) cells and canine fibroma cells (A72) displayed characteristic apoptotic events. Apoptosis was further confirmed by activation of caspases and cellular DNA damage. However, results from annexin V-propidium iodide (PI) labeling and membrane polarization assays indicated disruption of the plasma membrane uncommon to apoptosis. These results provide evidence that secondary necrosis followed apoptosis. In addition, two human cancer cell lines were found to be infected by CPV. This necrotic event over apoptotic cell death and infection in human cells provide insightful information when developing CPV as a nanotool for cancer treatments.
dc.language.isoeng
dc.publisherDove Medical Press Ltd
dc.relation.ispartofseriesInternational Journal of Nanomedicine
dc.relation.urihttp://www.dovepress.com/international-journal-of-nanomedicine-journal
dc.subject.otherkoiran parvovirus
dc.subject.otherapoptoosi
dc.subject.othernekroosi
dc.subject.othernanopartikkeli
dc.subject.othercanine parvovirus
dc.subject.otherapoptosis
dc.subject.othernecrosis
dc.subject.othernanoparticle
dc.titleMechanisms of cell death in canine parvovirus-infected cells provide intuitive insights to developing nanotools for medicine
dc.typearticle
dc.identifier.urnURN:NBN:fi:jyu-201511023572
dc.contributor.laitosBio- ja ympäristötieteiden laitosfi
dc.contributor.laitosDepartment of Biological and Environmental Scienceen
dc.contributor.oppiaineSolu- ja molekyylibiologiafi
dc.contributor.oppiaineCell and Molecular Biologyen
dc.type.urihttp://purl.org/eprint/type/JournalArticle
dc.date.updated2015-11-02T13:15:04Z
dc.type.coarhttp://purl.org/coar/resource_type/c_2df8fbb1
dc.description.reviewstatuspeerReviewed
dc.format.pagerange417-428
dc.relation.issn1178-2013
dc.relation.volume5
dc.type.versionpublishedVersion
dc.rights.copyright© 2010 Nykky et al, publisher and licensee Dove Medical Press Ltd. This is an Open Access article which permits unrestricted noncommercial use, provided the original work is properly cited.
dc.rights.accesslevelopenAccessfi
dc.subject.ysoviroterapia
jyx.subject.urihttp://www.yso.fi/onto/yso/p21936
dc.rights.urlhttps://creativecommons.org/licenses/by-nc/3.0/us/
dc.relation.doi10.2147/ijn.s10579
dc.type.okmA1


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Näytä suppeat kuvailutiedot

© 2010 Nykky et al, publisher and licensee Dove Medical Press Ltd. This is an Open Access article
which permits unrestricted noncommercial use, provided the original work is properly cited.
Ellei muuten mainita, aineiston lisenssi on © 2010 Nykky et al, publisher and licensee Dove Medical Press Ltd. This is an Open Access article which permits unrestricted noncommercial use, provided the original work is properly cited.