dc.contributor.author | Nykky, Jonna | |
dc.contributor.author | Tuusa, Jenni | |
dc.contributor.author | Kirjavainen, Sanna | |
dc.contributor.author | Vuento, Matti | |
dc.contributor.author | Gilbert, Leona | |
dc.date.accessioned | 2015-11-06T09:21:19Z | |
dc.date.available | 2015-11-06T09:21:19Z | |
dc.date.issued | 2010 | |
dc.identifier.citation | Nykky, J., Tuusa, J., Kirjavainen, S., Vuento, M., & Gilbert, L. (2010). Mechanisms of cell death in canine parvovirus-infected cells provide intuitive insights to developing nanotools for medicine. <i>International Journal of Nanomedicine</i>, <i>5</i>, 417-428. <a href="https://doi.org/10.2147/ijn.s10579" target="_blank">https://doi.org/10.2147/ijn.s10579</a> | |
dc.identifier.other | CONVID_19584480 | |
dc.identifier.other | TUTKAID_41069 | |
dc.identifier.uri | https://jyx.jyu.fi/handle/123456789/47581 | |
dc.description.abstract | Viruses have great potential as nanotools in medicine for gene transfer, targeted
gene delivery, and oncolytic cancer virotherapy. Here we have studied cell death mechanisms of
canine parvovirus (CPV) to increase the knowledge on the CPV life cycle in order to facilitate
the development of better parvovirus vectors. Morphological studies of CPV-infected Norden
laboratory feline kidney (NLFK) cells and canine fibroma cells (A72) displayed characteristic
apoptotic events. Apoptosis was further confirmed by activation of caspases and cellular DNA
damage. However, results from annexin V-propidium iodide (PI) labeling and membrane
polarization assays indicated disruption of the plasma membrane uncommon to apoptosis.
These results provide evidence that secondary necrosis followed apoptosis. In addition, two
human cancer cell lines were found to be infected by CPV. This necrotic event over apoptotic
cell death and infection in human cells provide insightful information when developing CPV
as a nanotool for cancer treatments. | |
dc.language.iso | eng | |
dc.publisher | Dove Medical Press Ltd | |
dc.relation.ispartofseries | International Journal of Nanomedicine | |
dc.relation.uri | http://www.dovepress.com/international-journal-of-nanomedicine-journal | |
dc.subject.other | koiran parvovirus | |
dc.subject.other | apoptoosi | |
dc.subject.other | nekroosi | |
dc.subject.other | nanopartikkeli | |
dc.subject.other | canine parvovirus | |
dc.subject.other | apoptosis | |
dc.subject.other | necrosis | |
dc.subject.other | nanoparticle | |
dc.title | Mechanisms of cell death in canine parvovirus-infected cells provide intuitive insights to developing nanotools for medicine | |
dc.type | article | |
dc.identifier.urn | URN:NBN:fi:jyu-201511023572 | |
dc.contributor.laitos | Bio- ja ympäristötieteiden laitos | fi |
dc.contributor.laitos | Department of Biological and Environmental Science | en |
dc.contributor.oppiaine | Solu- ja molekyylibiologia | fi |
dc.contributor.oppiaine | Cell and Molecular Biology | en |
dc.type.uri | http://purl.org/eprint/type/JournalArticle | |
dc.date.updated | 2015-11-02T13:15:04Z | |
dc.type.coar | http://purl.org/coar/resource_type/c_2df8fbb1 | |
dc.description.reviewstatus | peerReviewed | |
dc.format.pagerange | 417-428 | |
dc.relation.issn | 1178-2013 | |
dc.relation.volume | 5 | |
dc.type.version | publishedVersion | |
dc.rights.copyright | © 2010 Nykky et al, publisher and licensee Dove Medical Press Ltd. This is an Open Access article
which permits unrestricted noncommercial use, provided the original work is properly cited. | |
dc.rights.accesslevel | openAccess | fi |
dc.subject.yso | viroterapia | |
jyx.subject.uri | http://www.yso.fi/onto/yso/p21936 | |
dc.rights.url | https://creativecommons.org/licenses/by-nc/3.0/us/ | |
dc.relation.doi | 10.2147/ijn.s10579 | |
dc.type.okm | A1 | |