PGC-1 isoforms and their target genes are expressed differently in human skeletal muscle following resistance and endurance exercise
Silvennoinen, M., Ahtiainen, J., Hulmi, J., Pekkala, S., Taipale, R., Nindl, B. C., Laine, T., Häkkinen, K., Selänne, H., Kyröläinen, H., & Kainulainen, H. (2015). PGC-1 isoforms and their target genes are expressed differently in human skeletal muscle following resistance and endurance exercise. Physiological Reports, 3(10), Article e12563. https://doi.org/10.14814/phy2.12563
Published in
Physiological ReportsAuthors
Date
2015Discipline
LiikuntafysiologiaValmennus- ja testausoppiLiikuntalääketiedeExercise PhysiologyScience of Sport Coaching and Fitness TestingSports and Exercise MedicineCopyright
© 2015 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of
the American Physiological Society and The Physiological Society. This is an open access article under the terms of the Creative Commons Attribution License.
The primary aim of the present study was to investigate the acute gene expression
responses of PGC-1 isoforms and PGC-1a target genes related to mitochondrial
biogenesis (cytochrome C), angiogenesis (VEGF-A), and muscle
hypertrophy (myostatin), after a resistance or endurance exercise bout. In
addition, the study aimed to elucidate whether the expression changes of studied
transcripts were linked to phosphorylation of AMPK and MAPK p38.
Nineteen physically active men were divided into resistance exercise (RE,
n = 11) and endurance exercise (EE, n = 8) groups. RE group performed leg
press exercise (10 9 10 RM, 50 min) and EE walked on a treadmill (~80%
HRmax, 50 min). Muscle biopsies were obtained from the vastus lateralis muscle
before, 30 min, and 180 min after exercise. EE and RE significantly
increased the gene expression of alternative promoter originated PGC-1a exon
1b- and 1b’-derived isoforms, whereas the proximal promoter originated exon
1a-derived transcripts were less inducible and were upregulated only after EE.
Truncated PGC-1a transcripts were upregulated both after EE and RE. Neither
RE nor EE affected the expression of PGC-1b. EE upregulated the expression
of cytochrome C and VEGF-A, whereas RE upregulated VEGF-A and downregulated
myostatin. Both EE and RE increased the levels of p-AMPK and pMAPK
p38, but these changes were not linked to the gene expression
responses of PGC-1 isoforms. The present study comprehensively assayed
PGC-1 transcripts in human skeletal muscle and showed exercise mode-speci-
fic responses thus improving the understanding of early signaling events in
exercise-induced muscle adaptations.
...
Publisher
Wiley Periodicals, Inc.; American Physiological Society and The Physiological SocietyISSN Search the Publication Forum
2051-817XKeywords
Publication in research information system
https://converis.jyu.fi/converis/portal/detail/Publication/25210951
Metadata
Show full item recordCollections
- Liikuntatieteiden tiedekunta [2895]
License
Except where otherwise noted, this item's license is described as © 2015 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of
the American Physiological Society and The Physiological Society. This is an open access article under the terms of the Creative Commons Attribution License.
Related items
Showing items with similar title or keywords.
-
Effects of resistance training on expression of IGF-I splice variants in younger and older men
Ahtiainen, Juha; Hulmi, Juha; Lehti, Maarit; Kraemer, William J.; Nyman, Kai; Selänne, Harri; Alen, Markku; Komulainen, Jyrki; Kovanen, Vuokko; Mero, Antti; Philippou, Anastassios; Laakkonen, Eija; Häkkinen, Keijo (Taylor & Francis Ltd.; European College of Sport Science, 2016)Insulin-like growth factor-I (IGF-I) and its splice variants Insulin-like growth factor-I isoform Ea (IGF-IEa) and mechano growth factor (MGF) may play an important role in muscular adaptations to resistance training (RT) ... -
No Difference in Penetrance between Truncating and Missense/Aberrant Splicing Pathogenic Variants in MLH1 and MSH2 : A Prospective Lynch Syndrome Database Study
Dominguez-Valentin, Mev; Plazzer, John-Paul; Sampson, Julian R.; Engel, Christoph; Aretz, Stefan; Jenkins, Mark A.; Sunde, Lone; Bernstein, Inge; Capella, Gabriel; Balaguer, Francesc; Macrae, Finlay; Winship, Ingrid M.; Thomas, Huw; Evans, Dafydd Gareth; Burn, John; Greenblatt, Marc; de Vos tot Nederveen Cappel, Wouter H.; Sijmons, Rolf H.; Nielsen, Maartje; Bertario, Lucio; Bonanni, Bernardo; Tibiletti, Maria Grazia; Cavestro, Giulia Martina; Lindblom, Annika; Della Valle, Adriana; Lopez-Kostner, Francisco; Alvarez, Karin; Gluck, Nathan; Katz, Lior; Heinimann, Karl; Vaccaro, Carlos A.; Nakken, Sigve; Hovig, Eivind; Green, Kate; Lalloo, Fiona; Hill, James; Vasen, Hans F. A.; Perne, Claudia; Büttner, Reinhard; Görgens, Heike; Holinski-Feder, Elke; Morak, Monika; Holzapfel, Stefanie; Hüneburg, Robert; von Knebel Doeberitz, Magnus; Loeffler, Markus; Rahner, Nils; Weitz, Jürgen; Steinke-Lange, Verena; Schmiegel, Wolff; Vangala, Deepak; Crosbie, Emma J.; Pineda, Marta; Navarro, Matilde; Brunet, Joan; Moreira, Leticia; Sánchez, Ariadna; Serra-Burriel, Miquel; Mints, Miriam; Kariv, Revital; Rosner, Guy; Piñero, Tamara Alejandra; Pavicic, Walter Hernán; Kalfayan, Pablo; Broeke, Sanne W. ten; Mecklin, Jukka-Pekka; Pylvänäinen, Kirsi; Renkonen-Sinisalo, Laura; Lepistö, Anna; Peltomäki, Päivi; Hopper, John L.; Win, Aung Ko; Buchanan, Daniel D.; Lindor, Noralane M.; Gallinger, Steven; Le Marchand, Loïc; Newcomb, Polly A.; Figueiredo, Jane C.; Thibodeau, Stephen N.; Therkildsen, Christina; Hansen, Thomas V. O.; Lindberg, Lars; Rødland, Einar Andreas; Neffa, Florencia; Esperon, Patricia; Tjandra, Douglas; Möslein, Gabriela; Seppälä, Toni T.; Møller, Pål (MDPI AG, 2021)Background. Lynch syndrome is the most common genetic predisposition for hereditary cancer. Carriers of pathogenic changes in mismatch repair (MMR) genes have an increased risk of developing colorectal (CRC), endometrial, ... -
Stimuli and sensors that initiate skeletal muscle hypertrophy following resistance exercise
Wackerhage, Henning; Schoenfeld, Brad J.; Hamilton, D. Lee; Lehti, Maarit; Hulmi, Juha (American Physiological Society, 2019)One of the most striking adaptations to exercise is the skeletal muscle hypertrophy that occurs in response to resistance exercise. A large body of work shows that a mammalian target of rapamycin complex 1 (mTORC1)-mediated ... -
Are skeletal muscle FNDC5 expression and irisin release regulated by exercise and related to health?
Pekkala, Satu; Wiklund, Petri; Hulmi, Juha; Ahtiainen, Juha; Horttanainen, Mia; Laakkonen, Eija; Mäkelä, Kari; Kainulainen, Heikki; Häkkinen, Keijo; Nyman, Kai; Alén, Markku; Herzig, Karl-Heinz; Cheng, Sulin (Wiley-Blackwell, 2013)Recently, contradictory findings have been reported concerning the function of irisin and its precursor gene, skeletal muscle FNDC5, in energy homeostasis, and the associated regulatory role of exercise and PGC-1α. We ... -
Active recovery shows favorable IGF-I and IGF binding protein responses following heavy resistance exercise compared to passive recovery
Taipale, R. S.; Gagnon, S. S.; Ahtiainen, J. P.; Häkkinen, K.; Kyröläinen, H.; Nindl, B.C. (Elsevier Ltd, 2019)IGF-I and IGFBPs have important physiological modulatory effects and this study sought to examine the influence of active vs. passive recovery following a heavy resistance exercise on IGF-I and IGF binding protein (IGFBP) ...