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dc.contributor.authorMoscoso, Carlos G.
dc.contributor.authorXing, Li
dc.contributor.authorHui, Jinwen
dc.contributor.authorHu, Jeffrey
dc.contributor.authorKalkhoran, Mohammad Baikoghli
dc.contributor.authorYenigun, Onur M.
dc.contributor.authorSun, Yide
dc.contributor.authorPaavolainen, Lassi
dc.contributor.authorMartin, Loïc
dc.contributor.authorVahlne, Anders
dc.contributor.authorZambonelli, Carlo
dc.contributor.authorBarnett, Susan W.
dc.contributor.authorSrivastava, Indresh K.
dc.contributor.authorCheng, R. Holland
dc.date.accessioned2014-12-12T08:37:57Z
dc.date.available2014-12-12T08:37:57Z
dc.date.issued2014
dc.identifier.citationMoscoso, C. G., Xing, L., Hui, J., Hu, J., Kalkhoran, M. B., Yenigun, O. M., Sun, Y., Paavolainen, L., Martin, L., Vahlne, A., Zambonelli, C., Barnett, S. W., Srivastava, I. K., & Cheng, R. H. (2014). Trimeric HIV Env provides epitope occlusion mediated by hypervariable loops. <i>Scientific reports</i>, <i>4</i>(November), Article 7025. <a href="https://doi.org/10.1038/srep07025" target="_blank">https://doi.org/10.1038/srep07025</a>
dc.identifier.otherCONVID_24027436
dc.identifier.otherTUTKAID_63932
dc.identifier.urihttps://jyx.jyu.fi/handle/123456789/44858
dc.description.abstractHypervariable loops of HIV-1 Env protein gp120 are speculated to play roles in the conformational transition of Env to the receptor binding-induced metastable state. Structural analysis of full-length Env-based immunogens, containing the entire V2 loop, displayed tighter association between gp120 subunits, resulting in a smaller trimeric diameter than constructs lacking V2. A prominent basal quaternary location of V2 and V39 that challenges previous reports would facilitate gp41-independent gp120-gp120 interactions and suggests a quaternary mechanism of epitope occlusion facilitated by hypervariable loops. Deletion of V2 resulted in dramatic exposure of basal, membrane-proximal gp41 epitopes, consistent with its predicted basal location. The structural features of HIV-1 Env characterized here provide grounds for a paradigm shift in loop exposure and epitope occlusion, while providing substantive rationale for epitope display required for elicitation of broadly neutralizing antibodies, as well as substantiating previous pertinent literature disregarded in recent reports.
dc.language.isoeng
dc.publisherNature Publishing Group
dc.relation.ispartofseriesScientific reports
dc.relation.urihttp://www.nature.com/srep/2014/141114/srep07025/full/srep07025.html
dc.subject.otherhypervariable loops
dc.subject.otherHIV-1
dc.subject.otherEnv
dc.subject.otherprotein
dc.subject.othergp120
dc.titleTrimeric HIV Env provides epitope occlusion mediated by hypervariable loops
dc.typearticle
dc.identifier.urnURN:NBN:fi:jyu-201412113482
dc.contributor.laitosBio- ja ympäristötieteiden laitosfi
dc.contributor.laitosDepartment of Biological and Environmental Scienceen
dc.contributor.oppiaineSolu- ja molekyylibiologiafi
dc.contributor.oppiaineNanoscience Centerfi
dc.contributor.oppiaineCell and Molecular Biologyen
dc.contributor.oppiaineNanoscience Centeren
dc.type.urihttp://purl.org/eprint/type/JournalArticle
dc.date.updated2014-12-11T16:30:07Z
dc.type.coarjournal article
dc.description.reviewstatuspeerReviewed
dc.relation.issn2045-2322
dc.relation.numberinseriesNovember
dc.relation.volume4
dc.type.versionpublishedVersion
dc.rights.copyright© the Authors. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material.
dc.rights.accesslevelopenAccessfi
dc.rights.urlhttp://creativecommons.org/licenses/by/4.0/
dc.relation.doi10.1038/srep07025


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© the Authors. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material.
Except where otherwise noted, this item's license is described as © the Authors. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material.