Trimeric HIV Env provides epitope occlusion mediated by hypervariable loops
Moscoso, C. G., Xing, L., Hui, J., Hu, J., Kalkhoran, M. B., Yenigun, O. M., Sun, Y., Paavolainen, L., Martin, L., Vahlne, A., Zambonelli, C., Barnett, S. W., Srivastava, I. K., & Cheng, R. H. (2014). Trimeric HIV Env provides epitope occlusion mediated by hypervariable loops. Scientific reports, 4(November), Article 7025. https://doi.org/10.1038/srep07025
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Xing, Li |
Date
2014Copyright
© the Authors. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material.
Hypervariable loops of HIV-1 Env protein gp120 are speculated to play roles in the conformational transition of Env to the receptor binding-induced metastable state. Structural analysis of full-length Env-based immunogens, containing the entire V2 loop, displayed tighter association between gp120 subunits, resulting in a smaller trimeric diameter than constructs lacking V2. A prominent basal quaternary location of V2 and V39 that challenges previous reports would facilitate gp41-independent gp120-gp120
interactions and suggests a quaternary mechanism of epitope occlusion facilitated by hypervariable loops. Deletion of V2 resulted in dramatic exposure of basal, membrane-proximal gp41 epitopes, consistent with its predicted basal location. The structural features of HIV-1 Env characterized here provide grounds for a paradigm shift in loop exposure and epitope occlusion, while providing substantive rationale for epitope
display required for elicitation of broadly neutralizing antibodies, as well as substantiating previous pertinent literature disregarded in recent reports.
...
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Nature Publishing GroupISSN Search the Publication Forum
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http://www.nature.com/srep/2014/141114/srep07025/full/srep07025.htmlPublication in research information system
https://converis.jyu.fi/converis/portal/detail/Publication/24027436
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Except where otherwise noted, this item's license is described as © the Authors. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material.
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