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dc.contributor.authorSahoo, Tarini Prasad
dc.contributor.authorOikari, Aimo
dc.date.accessioned2014-08-18T06:07:26Z
dc.date.available2014-08-18T06:07:26Z
dc.date.issued2013
dc.identifier.citationSahoo, T. P., & Oikari, A. (2013). Use of Early Juvenile Zebrafish Danio Rerio for In-Vivo Assessment of Endocrine Modulation by Xenoestrogens. <i>Journal of Environmental and Analytical Toxicology</i>, <i>4</i>(1), 1-13. <a href="https://doi.org/10.4172/2161-0525.1000202" target="_blank">https://doi.org/10.4172/2161-0525.1000202</a>
dc.identifier.otherCONVID_23713931
dc.identifier.otherTUTKAID_62087
dc.identifier.urihttps://jyx.jyu.fi/handle/123456789/44035
dc.description.abstractReliable and cost-effective early-life stage (ELS) bioassays incorporating practical experimentation without compromising scientific relevance are crucial in chemical risk assessment. This study investigated the use of 20 days- post-fertilization life stage ( 20dpfZF ) of zebrafish Danio rerio to screen environmental chemicals known to be estrogenic in adult fish. Firstly, studies with key genes in steroidogenesis were conducted; the brain isoform of aromatase gene ( cyp19a1b ) being the most prominently expressed biomarker. Regulation of mRNA levels of molecular biomarkers, vitellogenin 1 gene ( vtg1) and cyp19a1b were selected to assess the endocrine modulation by xenoestrogens, 17α-ethinylestradiol (EE2), 4- n -nonylphenol (NP), 4- t -octylphenol (OP) and bisphenol A (BPA). Groups of 20dpfZF (n=15) as three replicates were exposed to chemicals over a five-day period in aerated static setups. Exposure of 20dpfZF to sediment spiked with EE2 (nominal 3μg g -1 dw) was also conducted to assess the sensitivity of this life-stage to sediment with estrogenic potency. Whole body homogenates of exposed juveniles showed the estrogenic potential of chemicals in the order: EE2 > OP > BPA > NP. Higher relative expression of cyp19a1b was noticed at lower ambient concentrations of EE2, although vtg1 showed more pronounced expression to it. The 20dpfZF responded in a dose-related way to sediment spiked with EE2, expanding its use as a general aquatic animal model. The suitability of 20dpfZF as an in vivo model, along with stable expression of reference genes was established. In addition to consistent expression pattern of key target genes on xenoestrogenicity, it serves as a practical screening model for the risk assessment of environmental chemicals and samples with estrogenic potential.fi
dc.language.isoeng
dc.publisherOMICS Publishing Group
dc.relation.ispartofseriesJournal of Environmental and Analytical Toxicology
dc.relation.urihttp://omicsonline.org/use-of-early-juvenile-zebrafish-danio-rerio-for-invivo-assessment-of-endocrine-modulation-by-xenoestrogens-2161-0525.1000202.pdf
dc.subject.otherbrain aromatase
dc.subject.otherearly life stage
dc.subject.otherVitellogenin 1
dc.subject.otherXenoestrogenicity
dc.subject.otherZebrafish
dc.titleUse of Early Juvenile Zebrafish Danio Rerio for In-Vivo Assessment of Endocrine Modulation by Xenoestrogens
dc.typearticle
dc.identifier.urnURN:NBN:fi:jyu-201407232249
dc.contributor.laitosBio- ja ympäristötieteiden laitosfi
dc.contributor.laitosDepartment of Biological and Environmental Scienceen
dc.contributor.oppiaineYmpäristötiedefi
dc.contributor.oppiaineEnvironmental Scienceen
dc.type.urihttp://purl.org/eprint/type/JournalArticle
dc.date.updated2014-07-23T03:30:03Z
dc.type.coarjournal article
dc.description.reviewstatuspeerReviewed
dc.format.pagerange1-13
dc.relation.issn2161-0525
dc.relation.numberinseries1
dc.relation.volume4
dc.type.versionpublishedVersion
dc.rights.copyright© 2013 Sahoo TP, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
dc.rights.accesslevelopenAccessfi
dc.rights.urlhttps://creativecommons.org/licenses/by/3.0/
dc.relation.doi10.4172/2161-0525.1000202


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© 2013 Sahoo TP, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Except where otherwise noted, this item's license is described as © 2013 Sahoo TP, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.