Use of Early Juvenile Zebrafish Danio Rerio for In-Vivo Assessment of Endocrine Modulation by Xenoestrogens
Sahoo, T. P., & Oikari, A. (2013). Use of Early Juvenile Zebrafish Danio Rerio for In-Vivo Assessment of Endocrine Modulation by Xenoestrogens. Journal of Environmental and Analytical Toxicology, 4(1), 1-13. https://doi.org/10.4172/2161-0525.1000202
Julkaistu sarjassa
Journal of Environmental and Analytical ToxicologyPäivämäärä
2013Tekijänoikeudet
© 2013 Sahoo TP, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Reliable and cost-effective early-life stage (ELS) bioassays incorporating practical experimentation without
compromising scientific relevance are crucial in chemical risk assessment. This study investigated the use of 20 days-
post-fertilization life stage (
20dpfZF
) of zebrafish
Danio rerio
to screen environmental chemicals known to be estrogenic
in adult fish. Firstly, studies with key genes in steroidogenesis were conducted; the brain isoform of aromatase gene
(
cyp19a1b
) being the most prominently expressed biomarker. Regulation of mRNA levels of molecular biomarkers,
vitellogenin 1 gene (
vtg1)
and
cyp19a1b
were selected to assess the endocrine modulation by xenoestrogens,
17α-ethinylestradiol (EE2), 4-
n
-nonylphenol (NP), 4-
t
-octylphenol (OP) and bisphenol A (BPA). Groups of
20dpfZF
(n=15)
as three replicates were exposed to chemicals over a five-day period in aerated static setups. Exposure of
20dpfZF
to
sediment spiked with EE2 (nominal 3μg g
-1
dw) was also conducted to assess the sensitivity of this life-stage to sediment
with estrogenic potency. Whole body homogenates of exposed juveniles showed the estrogenic potential of chemicals in
the order: EE2 > OP > BPA > NP. Higher relative expression of
cyp19a1b
was noticed at lower ambient concentrations
of EE2, although
vtg1
showed more pronounced expression to it. The
20dpfZF
responded in a dose-related way to
sediment spiked with EE2, expanding its use as a general aquatic animal model. The suitability of
20dpfZF
as an
in vivo
model, along with stable expression of reference genes was established. In addition to consistent expression pattern of
key target genes on xenoestrogenicity, it serves as a practical screening model for the risk assessment of environmental
chemicals and samples with estrogenic potential.
...
Julkaisija
OMICS Publishing GroupISSN Hae Julkaisufoorumista
2161-0525
Alkuperäislähde
http://omicsonline.org/use-of-early-juvenile-zebrafish-danio-rerio-for-invivo-assessment-of-endocrine-modulation-by-xenoestrogens-2161-0525.1000202.pdfJulkaisu tutkimustietojärjestelmässä
https://converis.jyu.fi/converis/portal/detail/Publication/23713931
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Ellei muuten mainita, aineiston lisenssi on © 2013 Sahoo TP, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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