Näytä suppeat kuvailutiedot

dc.contributor.authorJuuti-Uusitalo, Kati M
dc.contributor.authorKaukinen, Katri
dc.contributor.authorMäki, Markku
dc.contributor.authorTuimala, Jarno
dc.contributor.authorKainulainen, Heikki
dc.date.accessioned2012-11-22T09:25:16Z
dc.date.available2012-11-22T09:25:16Z
dc.date.issued2006fi
dc.identifier.citationJuuti-Uusitalo, K., Kaukinen, K., Mäki, M., Tuimala, J., & Kainulainen, H. (2006). Gene expression in TGFbeta-induced epithelial cell differentiation in a three-dimensional intestinal epithelial cell differentiation model. BMC Genomics, 7:279. doi:10.1186/1471-2164-7-279fi
dc.identifier.urihttp://dx.doi.org/10.1186/1471-2164-7-279
dc.identifier.urihttps://jyx.jyu.fi/handle/123456789/40406
dc.description.abstractBackground. The TGFβ1-induced signal transduction processes involved in growth and differentiation are only partly known. The three-dimensional epithelial differentiation model, in which T84 epithelial cells are induced to differentiate either with TGFβ1 or IMR-90 mesenchymal cell-secreted soluble factors, is previously shown to model epithelial cell differentiation seen in intestine. That model has not been used for large scale gene expression studies, such as microarray method. Therefore the gene expression changes were studied in undifferentiated and differentiated three-dimensional T84 cultures with cDNA microarray method in order to study the molecular changes and find new players in epithelial cell differentiation. Results. The expression of 372 genes out of 5188 arrayed sequences was significantly altered, and 47 of them were altered by both mediators. The data were validated and the altered genes are presented in ontology classes. For the genes tested the expressions in protein level were in accordance with the mRNA results. We also found 194 genes with no known function to be potentially important in epithelial cell differentiation. The mRNA expression changes induced by TGFβ1 were bigger than changes induced by soluble factors secreted by IMR-90 mesenchymal cells. The gene expression data was depicted in already known signaling pathway routes. Conclusion. Our results reveal potential new signaling pathways and several new genes affected by TGFβ in epithelial cell differentiation. The differentiation induced by TGFβ1 appears to be more potent than the differentiation induced by mesenchymal cells. This study indicates that our cell culture model is a suitable tool in studying regulatory mechanisms during epithelial cell differentiation in intestine. Furthermore the present results indicate that our model is a good tool for finding new players acting in the differentiation of epithelial cells.fi
dc.language.isoeng
dc.publisherBioMed Central (BMC)
dc.relation.ispartofseriesBMC Genomics
dc.relation.ispartofseries1471-2164
dc.subject.otherepiteelisolu
dc.subject.othererilaistuminen
dc.subject.otherTGF-beta
dc.subject.othergeenilastu
dc.subject.othergeenien ilmeneminen
dc.subject.otherepithelial cell
dc.subject.otherdifferentiation
dc.subject.otherTGB-beta
dc.subject.othermicroarray
dc.subject.othergene expression
dc.titleGene expression in TGFbeta-induced epithelial cell differentiation in a three-dimensional intestinal epithelial cell differentiation modelfi
dc.typeArticle
dc.identifier.urnURN:NBN:fi:jyu-201804202294
dc.contributor.laitosLiikuntabiologian laitosfi
dc.contributor.laitosDepartment of Biology of Physical Activityen
dc.type.urihttp://purl.org/eprint/type/JournalArticle
dc.identifier.doi10.1186/1471-2164-7-279
dc.date.updated2012-11-15T14:59:38Z
dc.type.coarjournal article
dc.description.reviewstatuspeerReviewed
dc.type.versionpublishedVersion
dc.rights.copyright© 2006 Juuti-Uusitalo et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
dc.rights.accesslevelopenAccessfi
dc.rights.urlhttp://creativecommons.org/licenses/by/2.0


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Näytä suppeat kuvailutiedot

© 2006 Juuti-Uusitalo et al; licensee BioMed Central Ltd. 


This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Ellei muuten mainita, aineiston lisenssi on © 2006 Juuti-Uusitalo et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.