Näytä suppeat kuvailutiedot

dc.contributor.authorRinnankoski-Tuikka, Rita
dc.contributor.authorSilvennoinen, Mika
dc.contributor.authorTorvinen, Sira
dc.contributor.authorHulmi, Juha J.
dc.contributor.authorLehti, Maarit
dc.contributor.authorKivelä, Riikka
dc.contributor.authorReunanen, Hilkka
dc.contributor.authorKainulainen, Heikki
dc.date.accessioned2012-11-16T08:40:34Z
dc.date.available2012-11-16T08:40:34Z
dc.date.issued2012
dc.identifier.citationRinnankoski-Tuikka, R., Silvennoinen, M., Torvinen, S., Hulmi, J., Lehti, M., Kivelä, R., . . . , & Kainulainen, H. (2012). Effects of high-fat diet and physical activity on pyruvate dehydrogenase kinase-4 in mouse skeletal muscle. Nutrition & Metabolism, 9 (53). doi:10.1186/1743-7075-9-53 Retrieved from http://www.nutritionandmetabolism.com/content/9/1/53/abstract
dc.identifier.urihttp://dx.doi.org/10.1186/1743-7075-9-53
dc.identifier.urihttps://jyx.jyu.fi/handle/123456789/40343
dc.description.abstractBackground. The expression of PDK4 is elevated by diabetes, fasting and other conditions associated with the switch from the utilization of glucose to fatty acids as an energy source. It is previously shown that peroxisome proliferator-activated receptor γ coactivator 1α (PGC-1α), a master regulator of energy metabolism, coactivates in cell lines pyruvate dehydrogenase kinase-4 (PDK4) gene expression via the estrogen-related receptor α (ERRα). We investigated the effects of long-term high-fat diet and physical activity on the expression of PDK4, PGC-1α and ERRα and the amount and function of mitochondria in skeletal muscle. Methods. Insulin resistance was induced by a high-fat (HF) diet for 19 weeks in C57BL/6 J mice, which were either sedentary or with access to running wheels. The skeletal muscle expression levels of PDK4, PGC-1α and ERRα were measured and the quality and quantity of mitochondrial function was assessed. Results. The HF mice were more insulin-resistant than the low-fat (LF) -fed mice. Upregulation of PDK4 and ERRα mRNA and protein levels were seen after the HF diet, and when combined with running even more profound effects on the mRNA expression levels were observed. Chronic HF feeding and voluntary running did not have significant effects on PGC-1α mRNA or protein levels. No remarkable difference was found in the amount or function of mitochondria. Conclusions. Our results support the view that insulin resistance is not mediated by the decreased qualitative or quantitative properties of mitochondria. Instead, the role of PDK4 should be contemplated as a possible contributor to high-fat diet-induced insulin resistance.
dc.language.isoeng
dc.publisherBioMed Central (BMC)
dc.relation.ispartofseriesNutrition & Metabolism
dc.subject.otherskeletal muscleen
dc.subject.othermitochondriaen
dc.subject.otherlipidsen
dc.subject.otherglucoseen
dc.subject.otherfuel switchingen
dc.subject.otherlihasfi
dc.subject.othermitokondriafi
dc.subject.otherlipiditfi
dc.subject.otherglukoosifi
dc.titleEffects of high-fat diet and physical activity on pyruvate dehydrogenase kinase-4 in mouse skeletal muscle
dc.typeArticle
dc.identifier.urnURN:NBN:fi:jyu-201804202269
dc.contributor.laitosBio- ja ympäristötieteiden laitosfi
dc.contributor.laitosLiikuntabiologian laitosfi
dc.contributor.laitosThe Department of Biological and Environmental Scienceen
dc.contributor.laitosDepartment of Biology of Physical Activityen
dc.type.urihttp://purl.org/eprint/type/JournalArticle
dc.identifier.doi10.1186/1743-7075-9-53
dc.date.updated2012-11-13T08:29:04Z
dc.rights.holderRita Rinnankoski-Tuikka et al.; licensee BioMed Central Ltd.
dc.type.coarjournal article
dc.description.reviewstatuspeerReviewed
dc.relation.issn1743-7075
dc.type.versionpublishedVersion
dc.rights.copyright© 2012 Rinnankoski-Tuikka et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
dc.rights.accesslevelopenAccessfi
dc.rights.urlhttp://creativecommons.org/licenses/by/2.0


Aineistoon kuuluvat tiedostot

Thumbnail
Thumbnail

Aineisto kuuluu seuraaviin kokoelmiin

Näytä suppeat kuvailutiedot

Rita Rinnankoski-Tuikka et al.; licensee BioMed Central Ltd.
Ellei muuten mainita, aineiston lisenssi on Rita Rinnankoski-Tuikka et al.; licensee BioMed Central Ltd.