dc.contributor.author | Mäkelä, Kati | |
dc.contributor.author | Laanto, Elina | |
dc.contributor.author | Sundberg, Lotta‐Riina | |
dc.date.accessioned | 2024-07-08T06:44:01Z | |
dc.date.available | 2024-07-08T06:44:01Z | |
dc.date.issued | 2024 | |
dc.identifier.citation | Mäkelä, K., Laanto, E., & Sundberg, L. (2024). Determinants in the phage life cycle : The dynamic nature of ssDNA phage FLiP and host interactions under varying environmental conditions and growth phases. <i>Environmental Microbiology</i>, <i>26</i>(7), Article e16670. <a href="https://doi.org/10.1111/1462-2920.16670" target="_blank">https://doi.org/10.1111/1462-2920.16670</a> | |
dc.identifier.other | CONVID_220957976 | |
dc.identifier.uri | https://jyx.jyu.fi/handle/123456789/96323 | |
dc.description.abstract | The influence of environmental factors on the interactions between phages and bacteria, particularly single-stranded DNA (ssDNA) phages, has been largely unexplored. In this study, we used Finnlakevirus FLiP, the first known ssDNA phage species with a lipid membrane, as our model phage. We examined the infectivity of FLiP with three Flavobacterium host strains, B330, B167 and B114. We discovered that FLiP infection is contingent on the host strain and conditions such as temperature and bacterial growth phase. FLiP can infect its hosts across a wide temperature range, but optimal phage replication varies with each host. We uncovered some unique aspects of phage infectivity: FLiP has limited infectivity in liquid-suspended cells, but it improves when cells are surface-attached. Moreover, FLiP infects stationary phase B167 and B114 cells more rapidly and efficiently than exponentially growing cells, a pattern not observed with the B330 host. We also present the first experimental evidence of endolysin function in ssDNA phages. The activity of FLiP's lytic enzymes was found to be condition-dependent. Our findings underscore the importance of studying phage ecology in contexts that are relevant to the environment, as both the host and the surrounding conditions can significantly alter the outcome of phage–host interactions. | en |
dc.format.mimetype | application/pdf | |
dc.language.iso | eng | |
dc.publisher | Wiley-Blackwell | |
dc.relation.ispartofseries | Environmental Microbiology | |
dc.rights | CC BY 4.0 | |
dc.title | Determinants in the phage life cycle : The dynamic nature of ssDNA phage FLiP and host interactions under varying environmental conditions and growth phases | |
dc.type | research article | |
dc.identifier.urn | URN:NBN:fi:jyu-202407085150 | |
dc.contributor.laitos | Bio- ja ympäristötieteiden laitos | fi |
dc.contributor.laitos | Department of Biological and Environmental Science | en |
dc.type.uri | http://purl.org/eprint/type/JournalArticle | |
dc.type.coar | http://purl.org/coar/resource_type/c_2df8fbb1 | |
dc.description.reviewstatus | peerReviewed | |
dc.relation.issn | 1462-2912 | |
dc.relation.numberinseries | 7 | |
dc.relation.volume | 26 | |
dc.type.version | publishedVersion | |
dc.rights.copyright | © 2024 The Author(s). Environmental Microbiology published by John Wiley & Sons Ltd. | |
dc.rights.accesslevel | openAccess | fi |
dc.type.publication | article | |
dc.relation.grantnumber | 101117204 | |
dc.relation.grantnumber | 101117204 | |
dc.relation.grantnumber | 346772 | |
dc.relation.projectid | info:eu-repo/grantAgreement/EC/H2020/101117204/EU//GIAPHAGE | |
dc.format.content | fulltext | |
dc.rights.url | https://creativecommons.org/licenses/by/4.0/ | |
dc.relation.doi | 10.1111/1462-2920.16670 | |
dc.relation.funder | European Commission | en |
dc.relation.funder | Research Council of Finland | en |
dc.relation.funder | Euroopan komissio | fi |
dc.relation.funder | Suomen Akatemia | fi |
jyx.fundingprogram | ERC Starting Grant, HE | en |
jyx.fundingprogram | Academy Project, AoF | en |
jyx.fundingprogram | ERC Starting Grant, HE | fi |
jyx.fundingprogram | Akatemiahanke, SA | fi |
jyx.fundinginformation | The research was funded by research grants from Emil Aaltonen Foundation (#200260, Lotta- Riina Sundberg), Research Council of Finland (#346772, Lotta-Riina Sundberg), Olvi Foundation (#201910409, Kati Mäkelä) and The Finnish Concordia Fund (#20200077, Kati Mäkelä). This project has received funding from the European Research Council (ERC) under the European Union’s Horizon Europe research and innovation programme (grant agreement No. 101117204) (Elina Laanto). | |
datacite.isSupplementedBy.doi | 10.17011/jyx/dataset/95450 | |
datacite.isSupplementedBy | Mäkelä, Kati; Laanto, Elina; Sundberg, Lotta-Riina. (2024). <i>Original data for article: “Determinants in the phage life cycle: The dynamic nature of ssDNA phage FLiP and host interactions under varying environmental conditions and growth phases”</i>. V. 14.5.2024. University of Jyväskylä. <a href="https://doi.org/10.17011/jyx/dataset/95450" target="_blank">https://doi.org/10.17011/jyx/dataset/95450</a>. <a href="http://urn.fi/URN:NBN:fi:jyu-202406034213">https://urn.fi/URN:NBN:fi:jyu-202406034213</a> | |
dc.type.okm | A1 | |