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dc.contributor.authorFranz, Ole
dc.contributor.authorHäkkänen, Heikki
dc.contributor.authorKovanen, Salla
dc.contributor.authorHeikkilä-Huhta, Kati
dc.contributor.authorNissinen, Riitta
dc.contributor.authorIhalainen, Janne A.
dc.date.accessioned2024-05-22T11:30:05Z
dc.date.available2024-05-22T11:30:05Z
dc.date.issued2024
dc.identifier.citationFranz, O., Häkkänen, H., Kovanen, S., Heikkilä-Huhta, K., Nissinen, R., & Ihalainen, J. A. (2024). NIRis: A low-cost, versatile imaging system for near-infrared fluorescence detection of phototrophic cell colonies used in research and education. <i>PLoS ONE</i>, <i>19</i>, Article e0287088. <a href="https://doi.org/10.1371/journal.pone.0287088" target="_blank">https://doi.org/10.1371/journal.pone.0287088</a>
dc.identifier.otherCONVID_215898115
dc.identifier.urihttps://jyx.jyu.fi/handle/123456789/95071
dc.description.abstractA variety of costly research-grade imaging devices are available for the detection of spectroscopic features. Here we present an affordable, open-source and versatile device, suitable for a range of applications. We provide the files to print the imaging chamber with commonly available 3D printers and instructions to assemble it with easily available hardware. The imager is suitable for rapid sample screening in research, as well as for educational purposes. We provide details and results for an already proven set-up which suits the needs of a research group and students interested in UV-induced near-infrared fluorescence detection of microbial colonies grown on Petri dishes. The fluorescence signal confirms the presence of bacteriochlorophyll a in aerobic anoxygenic phototrophic bacteria (AAPB). The imager allows for the rapid detection and subsequent isolation of AAPB colonies on Petri dishes with diverse environmental samples. To this date, 15 devices have been build and more than 7000 Petri dishes have been analyzed for AAPB, leading to over 1000 new AAPB isolates. Parts can be modified depending on needs and budget. The latest version with automated switches and double band pass filters costs around 350€ in materials and resolves bacterial colonies with diameters of 0.5 mm and larger. The low cost and modular build allow for the integration in high school classes to educate students on light properties, fluorescence and microbiology. Computer-aided design of 3D-printed parts and programming of the employed Raspberry Pi computer could be incorporated in computer sciences classes. Students have been also inspired to do agar art with microbes. The device is currently used in seven different high schools in Finland. Additionally, a science education network of Finnish universities has incorporated it in its program for high school students. Video guides have been produced to facilitate easy operation and accessibility of the device.en
dc.format.mimetypeapplication/pdf
dc.language.isoeng
dc.publisherPublic Library of Science (PLoS)
dc.relation.ispartofseriesPLoS ONE
dc.rightsCC BY 4.0
dc.subject.otherfluorescence imaging
dc.subject.otherfluorescence
dc.subject.otherartificial light
dc.subject.othernear-infrared spectroscopy
dc.subject.otherwhite light
dc.subject.other3D printing
dc.subject.othercomputer hardware
dc.subject.otherschools
dc.titleNIRis: A low-cost, versatile imaging system for near-infrared fluorescence detection of phototrophic cell colonies used in research and education
dc.typearticle
dc.identifier.urnURN:NBN:fi:jyu-202405223834
dc.contributor.laitosBio- ja ympäristötieteiden laitosfi
dc.contributor.laitosDepartment of Biological and Environmental Scienceen
dc.type.urihttp://purl.org/eprint/type/JournalArticle
dc.type.coarhttp://purl.org/coar/resource_type/c_2df8fbb1
dc.description.reviewstatuspeerReviewed
dc.relation.issn1932-6203
dc.relation.volume19
dc.type.versionpublishedVersion
dc.rights.copyright© 2024 Franz et al.
dc.rights.accesslevelopenAccessfi
dc.subject.yso3D-tulostus
dc.subject.ysofluoresenssi
dc.subject.ysokeinovalo
dc.subject.ysolähi-infrapunaspektroskopia
dc.format.contentfulltext
jyx.subject.urihttp://www.yso.fi/onto/yso/p27475
jyx.subject.urihttp://www.yso.fi/onto/yso/p3265
jyx.subject.urihttp://www.yso.fi/onto/yso/p11565
jyx.subject.urihttp://www.yso.fi/onto/yso/p27205
dc.rights.urlhttps://creativecommons.org/licenses/by/4.0/
dc.relation.doi10.1371/journal.pone.0287088
jyx.fundinginformationRN and JI received a joined funding from Kone foundation under the project name "Shared light". Funding number is 202009816.
dc.type.okmA1


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