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dc.contributor.authorLaine, Miikka Benjami
dc.contributor.authorMartin‐Creuzburg, Dominik
dc.contributor.authorLitmanen, Jaakko J.
dc.contributor.authorTaipale, Sami J.
dc.date.accessioned2024-03-14T13:11:05Z
dc.date.available2024-03-14T13:11:05Z
dc.date.issued2024
dc.identifier.citationLaine, M. B., Martin‐Creuzburg, D., Litmanen, J. J., & Taipale, S. J. (2024). Sterol limitation of Daphnia on eukaryotic phytoplankton : a combined supplementation and compound‐specific stable isotope labeling approach. <i>Oikos</i>, <i>Early View</i>. <a href="https://doi.org/10.1111/oik.10359" target="_blank">https://doi.org/10.1111/oik.10359</a>
dc.identifier.otherCONVID_207451280
dc.identifier.urihttps://jyx.jyu.fi/handle/123456789/93912
dc.description.abstractEssential biomolecules can critically influence the performance of consumers. A deficiency in dietary sterols has been shown to constrain the food quality of prokaryotic food sources for aquatic consumers. Here, we assessed the importance of dietary cholesterol for life history traits (survival, growth and egg production) of the freshwater herbivore Daphnia magna in supplementation experiments with various sterol-containing eukaryotic phytoplankton diets (dinoflagellates, diatoms, and golden algae). We combined cholesterol supplementation via liposomes with 13C-labelling of the phytoplankton diets and traced the origin of cholesterol in Daphnia using compound-specific stable isotope analysis. All phytoplankton strains used here were rich in polyunsaturated fatty acids (PUFA) but differed in their phytosterol composition. We show that growth and reproduction of D. magna can be limited by sterols even when feeding on sterol-containing eukaryotic phytoplankton diets. The impact of cholesterol supplementation on growth and reproduction of D. magna differed among phytoplankton diets (strains). The positive effect of cholesterol supplementation was most pronounced on diatom diets. Estimation of source proportions using stable isotopes revealed that D. magna preferentially assimilated the supplemented cholesterol rather than synthesizing it from dietary phytosterols. Our experiments suggest that the different responses to cholesterol supplementation on the various phytoplankton diets were unrelated to the suitability of dietary phytosterols to serve as cholesterol precursors but were caused by other biochemical or morphological food quality constraints. The combination of methods applied here could be very useful for uncovering nutritional constraints and thus for assessing the importance of essential biomolecules for the performance of herbivorous consumers.en
dc.format.mimetypeapplication/pdf
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofseriesOikos
dc.rightsCC BY 4.0
dc.subject.otherliposomes
dc.subject.othernutritional ecology
dc.subject.otherstable isotopes
dc.subject.othersterols
dc.subject.otherzooplankton
dc.titleSterol limitation of Daphnia on eukaryotic phytoplankton : a combined supplementation and compound‐specific stable isotope labeling approach
dc.typearticle
dc.identifier.urnURN:NBN:fi:jyu-202403142426
dc.contributor.laitosBio- ja ympäristötieteiden laitosfi
dc.contributor.laitosDepartment of Biological and Environmental Scienceen
dc.type.urihttp://purl.org/eprint/type/JournalArticle
dc.type.coarhttp://purl.org/coar/resource_type/c_2df8fbb1
dc.description.reviewstatuspeerReviewed
dc.relation.issn0030-1299
dc.relation.volumeEarly View
dc.type.versionpublishedVersion
dc.rights.copyright© 2024 the Authors
dc.rights.accesslevelopenAccessfi
dc.relation.grantnumber333564
dc.subject.ysoravitsemuskäyttäytyminen
dc.subject.ysoisotoopit
dc.subject.ysoekologia
dc.subject.ysoplankton
dc.subject.ysoliposomit
dc.subject.ysosterolit
dc.format.contentfulltext
jyx.subject.urihttp://www.yso.fi/onto/yso/p5528
jyx.subject.urihttp://www.yso.fi/onto/yso/p6387
jyx.subject.urihttp://www.yso.fi/onto/yso/p634
jyx.subject.urihttp://www.yso.fi/onto/yso/p3053
jyx.subject.urihttp://www.yso.fi/onto/yso/p38725
jyx.subject.urihttp://www.yso.fi/onto/yso/p4360
dc.rights.urlhttps://creativecommons.org/licenses/by/4.0/
dc.relation.doi10.1111/oik.10359
dc.relation.funderResearch Council of Finlanden
dc.relation.funderSuomen Akatemiafi
jyx.fundingprogramAcademy Project, AoFen
jyx.fundingprogramAkatemiahanke, SAfi
jyx.fundinginformationThis research was supported by the Academy of Finland grant 333564 awarded to SJT.
dc.type.okmA1


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