Characterization of a Type II L-Asparaginase from the Halotolerant Bacillus subtilis CH11
Arredondo-Nuñez, A., Monteiro, G., Flores-Fernández, C. N., Antenucci, L., Permi, P., & Zavaleta Amparo, I. (2023). Characterization of a Type II L-Asparaginase from the Halotolerant Bacillus subtilis CH11. Life, 13(11), Article 2145. https://doi.org/10.3390/life13112145
Julkaistu sarjassa
LifeTekijät
Päivämäärä
2023Oppiaine
Nanoscience CenterHyvinvoinnin tutkimuksen yhteisöBiologisten vuorovaikutusten huippututkimusyksikköNanoscience CenterSchool of WellbeingCentre of Excellence in Biological Interactions ResearchTekijänoikeudet
© 2023 by the authors. Licensee MDPI, Basel, Switzerland
L-asparaginases from bacterial sources have been used in antineoplastic treatments and the food industry. A type II L-asparaginase encoded by the N-truncated gene ansZP21 of halotolerant Bacillus subtilis CH11 isolated from Chilca salterns in Peru was expressed using a heterologous system in Escherichia coli BL21 (DE3)pLysS. The recombinant protein was purified using one-step nickel affinity chromatography and exhibited an activity of 234.38 U mg−1 and a maximum catalytic activity at pH 9.0 and 60 °C. The enzyme showed a homotetrameric form with an estimated molecular weight of 155 kDa through gel filtration chromatography. The enzyme half-life at 60 °C was 3 h 48 min, and L-asparaginase retained 50% of its initial activity for 24 h at 37 °C. The activity was considerably enhanced by KCl, CaCl2, MgCl2, mercaptoethanol, and DL-dithiothreitol (p-value < 0.01). Moreover, the Vmax and Km were 145.2 µmol mL−1 min−1 and 4.75 mM, respectively. These findings evidence a promising novel type II L-asparaginase for future industrial applications.
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2075-1729Asiasanat
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https://converis.jyu.fi/converis/portal/detail/Publication/194398464
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This research was partially funded by FONDECYT-CONCYTEC, grant number 169-2017. GM received grant from São Paulo Research Foundation (FAPESP—grant number 2022/02456-0) and a Productivity Fellowship from the Brazilian National Counsel of Technological and Scientific Development (CNPq 306060/2022-1).Lisenssi
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