Novel Methodology for Localizing and Studying Insect Dorsal Rim Area Morphology in 2D and 3D
Jie, V. W., Miettinen, A., & Baird, E. (2023). Novel Methodology for Localizing and Studying Insect Dorsal Rim Area Morphology in 2D and 3D. Insects, 14(8), Article 670. https://doi.org/10.3390/insects14080670
© 2023 by the authors. Licensee MDPI, Basel, Switzerland.
Polarized light-based navigation in insects is facilitated by a polarization-sensitive part of the eye, the dorsal rim area (DRA). Existing methods to study the anatomy of the DRA are destructive and time-consuming. We presented a novel method for DRA localization, dissection, and measurement using 3D volumetric images from X-ray micro-computed tomography in combination with 2D photographs. Applying the method on size-polymorphic buff-tailed bumblebees, Bombus terrestris, we found that the DRA was easily obtainable from photographs of the dorsal eye region. Allometric analysis of the DRA in relation to body size in B. terrestris showed that it increased with the body size but not at the same rate. By localizing the DRA of individual bumblebees, we could also perform individual-level descriptions and inter-individual comparisons between the ommatidial structures (lens, crystalline cones, rhabdoms) of three different eye regions (DRA, non-DRA, proximate to DRA). One feature distinct to the bumblebee DRA was the smaller dimension of the crystalline cones in comparison to other regions of the eye. Using our novel methodology, we provide the first individual-level description of DRA ommatidial features and a comparison of how the DRA varies with body size in bumblebees. ...
Dataset(s) related to the publicationhttps://doi.org/10.6084/m9.figshare.23628657
Publication in research information system
MetadataShow full item record
Additional information about fundingThis research was funded by Swedish Research Council (2018-06238).
Showing items with similar title or keywords.
Foraging Bumblebees Selectively Attend to Other Types of Bees Based on Their Reward-Predictive Value Romero-González, Jose E.; Royka, Amanda L.; MaBouDi, HaDi; Solvi, Cwyn; Seppänen, Janne-Tuomas; Loukola, Olli J. (MDPI, 2020)Using social information can be an efficient strategy for learning in a new environment while reducing the risks associated with trial-and-error learning. Whereas social information from conspecifics has long been assumed ...
Moradinour, Zahra; Wiklund, Christer; Jie, Vun Wen; Restrepo, Carlos E.; Gotthard, Karl; Miettinen, Arttu; Perl, Craig D.; Baird, Emily (MDPI AG, 2021)In solitary insect pollinators such as butterflies, sensory systems must be adapted for multiple tasks, including nectar foraging, mate-finding, and locating host-plants. As a result, the energetic investments between ...
Brander, Tommi; Ilmavirta, Joonas; Piiroinen, Petteri; Tyni, Teemu (American Institute of Mathematical Sciences (AIMS), 2020)We study an inverse problem where an unknown radiating source is observed with collimated detectors along a single line and the medium has a known attenuation. The research is motivated by applications in SPECT and beam ...
Characterization of spatial porosity and mineral distribution of crystalline rock using X-ray micro computed tomography, C-14-PMMA autoradiography and scanning electron microscopy Voutilainen, Mikko; Miettinen, Arttu; Sardini, Paul; Parkkonen, Joni; Sammaljärvi, Juuso; Gylling, Björn; Selroos, Jan-Olof; Yli-Kaila, Maarit; Koskinen, Lasse; Siitari-Kauppi, Marja (Pergamon, 2019)The spatial porosity and mineral distribution of geological materials strongly affects transport processes in them. X-ray micro computed tomography (X-μCT) has proven to be a powerful tool for characterizing the spatial ...
Romell, Jenny; Jie, Vun Wen; Miettinen, Arttu; Baird, Emily; Hertz, Hans M. (Wiley, 2021)Imaging the visual systems of bumblebees and other pollinating insects may increase understanding of their dependence on specific habitats and how they will be affected by climate change. Current high-resolution imaging ...