Näytä suppeat kuvailutiedot

dc.contributor.authorEriksson, Andreas N.M.
dc.contributor.authorRigaud, Cyril
dc.contributor.authorRokka, Anne
dc.contributor.authorSkaugen, Morten
dc.contributor.authorLihavainen, Jenna H.
dc.contributor.authorVehniäinen, Eeva-Riikka
dc.date.accessioned2022-05-25T05:20:30Z
dc.date.available2022-05-25T05:20:30Z
dc.date.issued2022
dc.identifier.citationEriksson, A. N., Rigaud, C., Rokka, A., Skaugen, M., Lihavainen, J. H., & Vehniäinen, E.-R. (2022). Changes in cardiac proteome and metabolome following exposure to the PAHs retene and fluoranthene and their mixture in developing rainbow trout alevins. <i>Science of the Total Environment</i>, <i>830</i>, Article 154846. <a href="https://doi.org/10.1016/j.scitotenv.2022.154846" target="_blank">https://doi.org/10.1016/j.scitotenv.2022.154846</a>
dc.identifier.otherCONVID_144402783
dc.identifier.urihttps://jyx.jyu.fi/handle/123456789/81270
dc.description.abstractExposure to polycyclic aromatic hydrocarbons (PAHs) is known to affect developing organisms. Utilization of different omics-based technologies and approaches could therefore provide a base for the discovery of novel mechanisms of PAH induced development of toxicity. To this aim, we investigated how exposure towards two PAHs with different toxicity mechanisms: retene (an aryl hydrocarbon receptor 2 (Ahr2) agonist), and fluoranthene (a weak Ahr2 agonist and cytochrome P450 inhibitor (Cyp1a)), either alone or as a mixture, affected the cardiac proteome and metabolome in newly hatched rainbow trout alevins (Oncorhynchus mykiss). In total, we identified 65 and 82 differently expressed proteins (DEPs) across all treatments compared to control (DMSO) after 7 and 14 days of exposure. Exposure to fluoranthene altered the expression of 11 and 19 proteins, retene 29 and 23, while the mixture affected 44 and 82 DEPs by Days 7 and 14, respectively. In contrast, only 5 significantly affected metabolites were identified. Pathway over-representation analysis identified exposure-specific activation of phase II metabolic processes, which were accompanied with exposure-specific body burden profiles. The proteomic data highlights that exposure to the mixture increased oxidative stress, altered iron metabolism and impaired coagulation capacity. Additionally, depletion of several mini-chromosome maintenance components, in combination with depletion of several intermediate filaments and microtubules, among alevins exposed to the mixture, suggests compromised cellular integrity and reduced rate of mitosis, whereby affecting heart growth and development. Furthermore, the combination of proteomic and metabolomic data indicates altered energy metabolism, as per amino acid catabolism among mixture exposed alevins; plausibly compensatory mechanisms as to counteract reduced absorption and consumption of yolk. When considered as a whole, proteomic and metabolomic data, in relation to apical effects on the whole organism, provides additional insight into PAH toxicity and the effects of exposure on heart structure and molecular processes.en
dc.format.mimetypeapplication/pdf
dc.language.isoeng
dc.publisherElsevier BV
dc.relation.ispartofseriesScience of the Total Environment
dc.rightsCC BY 4.0
dc.subject.otherPROTEOMICS
dc.subject.othermetabolomics
dc.subject.otherPAH
dc.subject.othermixture
dc.subject.otherdevelopmental toxicology
dc.subject.otherrainbow trout
dc.titleChanges in cardiac proteome and metabolome following exposure to the PAHs retene and fluoranthene and their mixture in developing rainbow trout alevins
dc.typeresearch article
dc.identifier.urnURN:NBN:fi:jyu-202205252898
dc.contributor.laitosBio- ja ympäristötieteiden laitosfi
dc.contributor.laitosDepartment of Biological and Environmental Scienceen
dc.contributor.oppiaineYmpäristötiedefi
dc.contributor.oppiaineEkologia ja evoluutiobiologiafi
dc.contributor.oppiaineEnvironmental Scienceen
dc.contributor.oppiaineEcology and Evolutionary Biologyen
dc.type.urihttp://purl.org/eprint/type/JournalArticle
dc.type.coarhttp://purl.org/coar/resource_type/c_2df8fbb1
dc.description.reviewstatuspeerReviewed
dc.relation.issn0048-9697
dc.relation.volume830
dc.type.versionpublishedVersion
dc.rights.copyright© 2022 The Authors. Published by Elsevier B.V.
dc.rights.accesslevelopenAccessfi
dc.type.publicationarticle
dc.relation.grantnumber294066
dc.relation.grantnumber285296
dc.relation.grantnumber319284
dc.subject.ysokehitysbiologia
dc.subject.ysoekotoksikologia
dc.subject.ysosydän
dc.subject.ysokirjolohi
dc.subject.ysoaineenvaihdunta
dc.subject.ysoproteomiikka
dc.subject.ysoPAH-yhdisteet
dc.format.contentfulltext
jyx.subject.urihttp://www.yso.fi/onto/yso/p18588
jyx.subject.urihttp://www.yso.fi/onto/yso/p19671
jyx.subject.urihttp://www.yso.fi/onto/yso/p16731
jyx.subject.urihttp://www.yso.fi/onto/yso/p12317
jyx.subject.urihttp://www.yso.fi/onto/yso/p3066
jyx.subject.urihttp://www.yso.fi/onto/yso/p7548
jyx.subject.urihttp://www.yso.fi/onto/yso/p10718
dc.rights.urlhttps://creativecommons.org/licenses/by/4.0/
dc.relation.doi10.1016/j.scitotenv.2022.154846
dc.relation.funderResearch Council of Finlanden
dc.relation.funderResearch Council of Finlanden
dc.relation.funderResearch Council of Finlanden
dc.relation.funderSuomen Akatemiafi
dc.relation.funderSuomen Akatemiafi
dc.relation.funderSuomen Akatemiafi
jyx.fundingprogramResearch costs of Academy Research Fellow, AoFen
jyx.fundingprogramAcademy Research Fellow, AoFen
jyx.fundingprogramResearch costs of Academy Research Fellow, AoFen
jyx.fundingprogramAkatemiatutkijan tutkimuskulut, SAfi
jyx.fundingprogramAkatemiatutkija, SAfi
jyx.fundingprogramAkatemiatutkijan tutkimuskulut, SAfi
jyx.fundinginformationAcademy of Finland project number: 285296, 294066 and 319284 granted to Eeva-Riikka Vehniäinen.
dc.type.okmA1


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