Näytä suppeat kuvailutiedot

dc.contributor.authorEriksson, Andreas N.M.
dc.contributor.authorRigaud, Cyril
dc.contributor.authorKrasnov, Aleksei
dc.contributor.authorWincent, Emma
dc.contributor.authorVehniäinen, Eeva-Riikka
dc.date.accessioned2022-02-02T06:58:58Z
dc.date.available2022-02-02T06:58:58Z
dc.date.issued2022
dc.identifier.citationEriksson, A. N., Rigaud, C., Krasnov, A., Wincent, E., & Vehniäinen, E.-R. (2022). Exposure to retene, fluoranthene, and their binary mixture causes distinct transcriptomic and apical outcomes in rainbow trout (Oncorhynchus mykiss) yolk sac alevins. <i>Aquatic Toxicology</i>, <i>244</i>, Article 106083. <a href="https://doi.org/10.1016/j.aquatox.2022.106083" target="_blank">https://doi.org/10.1016/j.aquatox.2022.106083</a>
dc.identifier.otherCONVID_104089906
dc.identifier.urihttps://jyx.jyu.fi/handle/123456789/79608
dc.description.abstractPolycyclic aromatic hydrocarbons (PAHs) are widely spread environmental contaminants which affect developing organisms. It is known that improper activation of the aryl hydrocarbon receptor (AhR) by some PAHs contributes to toxicity, while other PAHs can disrupt cellular membrane function. The exact downstream mechanisms of AhR activation remain unresolved, especially with regard to cardiotoxicity. By exposing newly hatched rainbow trout alevins (Oncorhynchus mykiss) semi-statically to retene (32 µg l−1; AhR agonist), fluoranthene (50 µg l−1; weak AhR agonist and CYP1a inhibitor) and their binary mixture for 1, 3, 7 and 14 days, we aimed to uncover novel mechanisms of cardiotoxicity using a targeted microarray approach. At the end of the exposure, standard length, yolk area, blue sac disease (BSD) index and PAH body burden were measured, while the hearts were prepared for microarray analysis. Each exposure produced a unique toxicity profile. We observed that retene and the mixture, but not fluoranthene, significantly reduced growth by Day 14 compared to the control, while exposure to the mixture increased the BSD-index significantly from Day 3 onward. Body burden profiles were PAH-specific and correlated well with the exposure-specific upregulations of genes encoding for phase I and II enzymes. Exposure to the mixture over-represented pathways related to growth, amino acid and xenobiotic metabolism and oxidative stress responses. Alevins exposed to the individual PAHs displayed over-represented pathways involved in receptor signaling: retene downregulated genes with a role in G-protein signaling, while fluoranthene upregulated those involved in GABA signaling. Furthermore, exposure to retene and fluoranthene altered the expression of genes encoding for proteins involved in calcium- and potassium ion channels, which suggests affected heart structure and function. This study provides deeper understanding of the complexity of PAH toxicity and the necessity of investigating PAHs as mixtures and not as individual components.en
dc.format.mimetypeapplication/pdf
dc.language.isoeng
dc.publisherElsevier
dc.relation.ispartofseriesAquatic Toxicology
dc.rightsCC BY 4.0
dc.subject.otherMixture
dc.subject.otherRetene
dc.subject.otherFluoranthene
dc.subject.otherPAH
dc.subject.otherOncorhynchus mykiss
dc.subject.otherEarly life development
dc.titleExposure to retene, fluoranthene, and their binary mixture causes distinct transcriptomic and apical outcomes in rainbow trout (Oncorhynchus mykiss) yolk sac alevins
dc.typearticle
dc.identifier.urnURN:NBN:fi:jyu-202202021370
dc.contributor.laitosBio- ja ympäristötieteiden laitosfi
dc.contributor.laitosDepartment of Biological and Environmental Scienceen
dc.contributor.oppiaineEkologia ja evoluutiobiologiafi
dc.contributor.oppiaineYmpäristötiedefi
dc.contributor.oppiaineEcology and Evolutionary Biologyen
dc.contributor.oppiaineEnvironmental Scienceen
dc.type.urihttp://purl.org/eprint/type/JournalArticle
dc.type.coarhttp://purl.org/coar/resource_type/c_2df8fbb1
dc.description.reviewstatuspeerReviewed
dc.relation.issn0166-445X
dc.relation.volume244
dc.type.versionpublishedVersion
dc.rights.copyright© 2022 The Author(s). Published by Elsevier B.V.
dc.rights.accesslevelopenAccessfi
dc.relation.grantnumber319284
dc.relation.grantnumber294066
dc.relation.grantnumber285296
dc.subject.ysokirjolohi
dc.subject.ysoympäristömyrkyt
dc.subject.ysoseokset
dc.subject.ysoekotoksikologia
dc.subject.ysoPAH-yhdisteet
dc.subject.ysoalkionkehitys
dc.subject.ysotranskriptomi
dc.format.contentfulltext
jyx.subject.urihttp://www.yso.fi/onto/yso/p12317
jyx.subject.urihttp://www.yso.fi/onto/yso/p2359
jyx.subject.urihttp://www.yso.fi/onto/yso/p13887
jyx.subject.urihttp://www.yso.fi/onto/yso/p19671
jyx.subject.urihttp://www.yso.fi/onto/yso/p10718
jyx.subject.urihttp://www.yso.fi/onto/yso/p7282
jyx.subject.urihttp://www.yso.fi/onto/yso/p38915
dc.rights.urlhttps://creativecommons.org/licenses/by/4.0/
dc.relation.doi10.1016/j.aquatox.2022.106083
dc.relation.funderResearch Council of Finlanden
dc.relation.funderResearch Council of Finlanden
dc.relation.funderResearch Council of Finlanden
dc.relation.funderSuomen Akatemiafi
dc.relation.funderSuomen Akatemiafi
dc.relation.funderSuomen Akatemiafi
jyx.fundingprogramResearch costs of Academy Research Fellow, AoFen
jyx.fundingprogramResearch costs of Academy Research Fellow, AoFen
jyx.fundingprogramAcademy Research Fellow, AoFen
jyx.fundingprogramAkatemiatutkijan tutkimuskulut, SAfi
jyx.fundingprogramAkatemiatutkijan tutkimuskulut, SAfi
jyx.fundingprogramAkatemiatutkija, SAfi
jyx.fundinginformationAcademy of Finland project number: 285296, 294066 and 319284 granted to Eeva-Riikka Vehniäinen.
dc.type.okmA1


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