Covalent and non-covalent coupling of a Au102 nanocluster with a fluorophore : energy transfer, quenching and intracellular pH sensing
Hulkko, E., Lahtinen, T., Marjomäki, V., Pohjolainen, E., Saarnio, V., Sokolowska, K., Ajitha, A., Kuisma, M., Lehtovaara, L., Groenhof, G., Häkkinen, H., & Pettersson, M. (2021). Covalent and non-covalent coupling of a Au102 nanocluster with a fluorophore : energy transfer, quenching and intracellular pH sensing. Nanoscale Advances, 3(23), 6649-6658. https://doi.org/10.1039/D1NA00368B
Published inNanoscale Advances
DisciplineOrgaaninen kemiaFysikaalinen kemiaSolu- ja molekyylibiologiaNanoscience CenterOrganic ChemistryPhysical ChemistryCell and Molecular BiologyNanoscience Center
© 2021 the Authors
Interactions between an atomically precise gold nanocluster Au102(p-MBA)44 (p-MBA = para mercaptobenzoic acid) and a fluorescent organic dye molecule (KU, azadioxatriangulenium) are studied. In solution, the constituents form spontaneously a weakly bound complex leading to quenching of fluorescence of the KU dye via energy transfer. The KU can be separated from the complex by lowering pH, leading to recovery of fluorescence, which forms a basis for an optical reversible pH sensor. However, the sensor is not a stable entity, which could be delivered inside cells. For this purpose, a covalently bound hybrid is synthesized by linking the KU dye to the ligand layer of the cluster via an ester bond. Covalent linking facilitates entry of the cluster–dye hybrids into cells via endocytosis. Inside cells, the hybrids accumulate in endosomes where Au102 releases its cargo via hydrolysis of the ester bond. Changes of the local pH inside endosomes regulate reversible fluorescence due to variations in the interactions between the Au102 cluster and the dye. This work presents a concept for delivering reporter molecules into cells by using atomically precise gold nanoclusters as carriers and paves the way for future developments of cluster-reporter sensors for in vivo measurements of e.g. absolute pH values or ion concentrations. ...
PublisherRoyal Society of Chemistry (RSC)
Publication in research information system
MetadataShow full item record
Related funder(s)Academy of Finland
Funding program(s)Research post as Academy Professor, AoF; Academy Project, AoF; Research costs of Academy Professor, AoF
Additional information about fundingThis work was supported by Academy of Finland (Grants 292352, 294217, 290677, 257125 to H. H., G. G. and V. M.) and Jane and Aatos Erkko foundation (V. M.).
Showing items with similar title or keywords.
Chemically Selective Imaging of Individual Bonds through Scanning Electron Energy-Loss Spectroscopy : Disulfide Bridges Linking Gold Nanoclusters Sokołowska, Karolina; Luan, Zhongyue; Hulkko, Eero; Rameshan, Christoph; Barrabés, Noelia; Apkarian, Vartkess A.; Lahtinen, Tanja (American Chemical Society, 2020)As proof-of-principle of chemically selective, spatially resolved imaging of individual bonds, we carry out electron energy-loss spectroscopy in a scanning transmission electron microscopy instrument on atomically precise, ...
Studies of Nature of Uncommon Bifurcated I–I···(I–M) Metal-Involving Noncovalent Interaction in Palladium(II) and Platinum(II) Isocyanide Cocrystals Bulatova, Margarita; Ivanov, Daniil M.; Rautiainen, J. Mikko; Kinzhalov, Mikhail A.; Truong, Khai-Nghi; Lahtinen, Manu; Haukka, Matti (American Chemical Society (ACS), 2021)Two isostructural trans-[MI2(CNXyl)2]·I2 (M = Pd or Pt; CNXyl = 2,6-dimethylphenyl isocyanide) metallopolymeric cocrystals containing uncommon bifurcated iodine···(metal–iodide) contact were obtained. In addition to classical ...
Synthesis, characterization, and reactivity of heavier group 13 and 14 metallylenes and metalloid clusters : small molecule activation and more Vasko, Petra (University of Jyväskylä, 2015)
Stark, Marie (University of Jyväskylä, 2017)Developments in diagnostic and therapeutic delivery are trending towards molecular level targeting with nano-platforms. Targeted delivery reduces generalized distribution by localizing diagnostic and/or therapeutic ...
Saarnio, Ville K.; Salorinne, Kirsi; Ruokolainen, Visa P.; Nilsson, Jesper R.; Tero, Tiia-Riikka; Oikarinen, Sami; Wilhelmsson, L. Marcus; Lahtinen, Tanja M.; Marjomäki, Varpu S. (Elsevier, 2020)Fluorescent probes for sensing nucleic acids have found widespread use in the field of cell and molecular biology. However, probes combined with potential for post-synthetic conjugation, e.g. for intra-endosomal measurements ...