dc.contributor.author | Hulkko, Eero | |
dc.contributor.author | Lahtinen, Tanja | |
dc.contributor.author | Marjomäki, Varpu | |
dc.contributor.author | Pohjolainen, Emmi | |
dc.contributor.author | Saarnio, Ville | |
dc.contributor.author | Sokolowska, Karolina | |
dc.contributor.author | Ajitha, Ardra | |
dc.contributor.author | Kuisma, Mikael | |
dc.contributor.author | Lehtovaara, Lauri | |
dc.contributor.author | Groenhof, Gerrit | |
dc.contributor.author | Häkkinen, Hannu | |
dc.contributor.author | Pettersson, Mika | |
dc.date.accessioned | 2021-11-08T07:39:26Z | |
dc.date.available | 2021-11-08T07:39:26Z | |
dc.date.issued | 2021 | |
dc.identifier.citation | Hulkko, E., Lahtinen, T., Marjomäki, V., Pohjolainen, E., Saarnio, V., Sokolowska, K., Ajitha, A., Kuisma, M., Lehtovaara, L., Groenhof, G., Häkkinen, H., & Pettersson, M. (2021). Covalent and non-covalent coupling of a Au102 nanocluster with a fluorophore : energy transfer, quenching and intracellular pH sensing. <i>Nanoscale Advances</i>, <i>3</i>(23), 6649-6658. <a href="https://doi.org/10.1039/D1NA00368B" target="_blank">https://doi.org/10.1039/D1NA00368B</a> | |
dc.identifier.other | CONVID_101761367 | |
dc.identifier.uri | https://jyx.jyu.fi/handle/123456789/78513 | |
dc.description.abstract | Interactions between an atomically precise gold nanocluster Au102(p-MBA)44 (p-MBA = para mercaptobenzoic acid) and a fluorescent organic dye molecule (KU, azadioxatriangulenium) are studied. In solution, the constituents form spontaneously a weakly bound complex leading to quenching of fluorescence of the KU dye via energy transfer. The KU can be separated from the complex by lowering pH, leading to recovery of fluorescence, which forms a basis for an optical reversible pH sensor. However, the sensor is not a stable entity, which could be delivered inside cells. For this purpose, a covalently bound hybrid is synthesized by linking the KU dye to the ligand layer of the cluster via an ester bond. Covalent linking facilitates entry of the cluster–dye hybrids into cells via endocytosis. Inside cells, the hybrids accumulate in endosomes where Au102 releases its cargo via hydrolysis of the ester bond. Changes of the local pH inside endosomes regulate reversible fluorescence due to variations in the interactions between the Au102 cluster and the dye. This work presents a concept for delivering reporter molecules into cells by using atomically precise gold nanoclusters as carriers and paves the way for future developments of cluster-reporter sensors for in vivo measurements of e.g. absolute pH values or ion concentrations. | en |
dc.format.mimetype | application/pdf | |
dc.language.iso | eng | |
dc.publisher | Royal Society of Chemistry (RSC) | |
dc.relation.ispartofseries | Nanoscale Advances | |
dc.rights | CC BY-NC 4.0 | |
dc.title | Covalent and non-covalent coupling of a Au102 nanocluster with a fluorophore : energy transfer, quenching and intracellular pH sensing | |
dc.type | article | |
dc.identifier.urn | URN:NBN:fi:jyu-202111085536 | |
dc.contributor.laitos | Kemian laitos | fi |
dc.contributor.laitos | Bio- ja ympäristötieteiden laitos | fi |
dc.contributor.laitos | Fysiikan laitos | fi |
dc.contributor.laitos | Department of Chemistry | en |
dc.contributor.laitos | Department of Biological and Environmental Science | en |
dc.contributor.laitos | Department of Physics | en |
dc.contributor.oppiaine | Orgaaninen kemia | fi |
dc.contributor.oppiaine | Fysikaalinen kemia | fi |
dc.contributor.oppiaine | Solu- ja molekyylibiologia | fi |
dc.contributor.oppiaine | Nanoscience Center | fi |
dc.contributor.oppiaine | Organic Chemistry | en |
dc.contributor.oppiaine | Physical Chemistry | en |
dc.contributor.oppiaine | Cell and Molecular Biology | en |
dc.contributor.oppiaine | Nanoscience Center | en |
dc.type.uri | http://purl.org/eprint/type/JournalArticle | |
dc.type.coar | http://purl.org/coar/resource_type/c_2df8fbb1 | |
dc.description.reviewstatus | peerReviewed | |
dc.format.pagerange | 6649-6658 | |
dc.relation.issn | 2516-0230 | |
dc.relation.numberinseries | 23 | |
dc.relation.volume | 3 | |
dc.type.version | publishedVersion | |
dc.rights.copyright | © 2021 the Authors | |
dc.rights.accesslevel | openAccess | fi |
dc.relation.grantnumber | 292352 | |
dc.relation.grantnumber | 290677 | |
dc.relation.grantnumber | 294217 | |
dc.subject.yso | fluoresenssi | |
dc.subject.yso | pH | |
dc.subject.yso | nanohiukkaset | |
dc.subject.yso | kemialliset sidokset | |
dc.subject.yso | väriaineet | |
dc.subject.yso | anturit | |
dc.format.content | fulltext | |
jyx.subject.uri | http://www.yso.fi/onto/yso/p3265 | |
jyx.subject.uri | http://www.yso.fi/onto/yso/p4555 | |
jyx.subject.uri | http://www.yso.fi/onto/yso/p23451 | |
jyx.subject.uri | http://www.yso.fi/onto/yso/p10130 | |
jyx.subject.uri | http://www.yso.fi/onto/yso/p2176 | |
jyx.subject.uri | http://www.yso.fi/onto/yso/p11460 | |
dc.rights.url | https://creativecommons.org/licenses/by-nc/4.0/ | |
dc.relation.doi | 10.1039/D1NA00368B | |
dc.relation.funder | Research Council of Finland | en |
dc.relation.funder | Research Council of Finland | en |
dc.relation.funder | Research Council of Finland | en |
dc.relation.funder | Suomen Akatemia | fi |
dc.relation.funder | Suomen Akatemia | fi |
dc.relation.funder | Suomen Akatemia | fi |
jyx.fundingprogram | Research post as Academy Professor, AoF | en |
jyx.fundingprogram | Academy Project, AoF | en |
jyx.fundingprogram | Research costs of Academy Professor, AoF | en |
jyx.fundingprogram | Akatemiaprofessorin tehtävä, SA | fi |
jyx.fundingprogram | Akatemiahanke, SA | fi |
jyx.fundingprogram | Akatemiaprofessorin tutkimuskulut, SA | fi |
jyx.fundinginformation | This work was supported by Academy of Finland (Grants 292352, 294217, 290677, 257125 to H. H., G. G. and V. M.) and Jane and Aatos Erkko foundation (V. M.). | |
dc.type.okm | A1 | |