Show simple item record

dc.contributor.authorHulkko, Eero
dc.contributor.authorLahtinen, Tanja
dc.contributor.authorMarjomäki, Varpu
dc.contributor.authorPohjolainen, Emmi
dc.contributor.authorSaarnio, Ville
dc.contributor.authorSokolowska, Karolina
dc.contributor.authorAjitha, Ardra
dc.contributor.authorKuisma, Mikael
dc.contributor.authorLehtovaara, Lauri
dc.contributor.authorGroenhof, Gerrit
dc.contributor.authorHäkkinen, Hannu
dc.contributor.authorPettersson, Mika
dc.date.accessioned2021-11-08T07:39:26Z
dc.date.available2021-11-08T07:39:26Z
dc.date.issued2021
dc.identifier.citationHulkko, E., Lahtinen, T., Marjomäki, V., Pohjolainen, E., Saarnio, V., Sokolowska, K., Ajitha, A., Kuisma, M., Lehtovaara, L., Groenhof, G., Häkkinen, H., & Pettersson, M. (2021). Covalent and non-covalent coupling of a Au102 nanocluster with a fluorophore : energy transfer, quenching and intracellular pH sensing. <i>Nanoscale Advances</i>, <i>3</i>(23), 6649-6658. <a href="https://doi.org/10.1039/D1NA00368B" target="_blank">https://doi.org/10.1039/D1NA00368B</a>
dc.identifier.otherCONVID_101761367
dc.identifier.urihttps://jyx.jyu.fi/handle/123456789/78513
dc.description.abstractInteractions between an atomically precise gold nanocluster Au102(p-MBA)44 (p-MBA = para mercaptobenzoic acid) and a fluorescent organic dye molecule (KU, azadioxatriangulenium) are studied. In solution, the constituents form spontaneously a weakly bound complex leading to quenching of fluorescence of the KU dye via energy transfer. The KU can be separated from the complex by lowering pH, leading to recovery of fluorescence, which forms a basis for an optical reversible pH sensor. However, the sensor is not a stable entity, which could be delivered inside cells. For this purpose, a covalently bound hybrid is synthesized by linking the KU dye to the ligand layer of the cluster via an ester bond. Covalent linking facilitates entry of the cluster–dye hybrids into cells via endocytosis. Inside cells, the hybrids accumulate in endosomes where Au102 releases its cargo via hydrolysis of the ester bond. Changes of the local pH inside endosomes regulate reversible fluorescence due to variations in the interactions between the Au102 cluster and the dye. This work presents a concept for delivering reporter molecules into cells by using atomically precise gold nanoclusters as carriers and paves the way for future developments of cluster-reporter sensors for in vivo measurements of e.g. absolute pH values or ion concentrations.en
dc.format.mimetypeapplication/pdf
dc.language.isoeng
dc.publisherRoyal Society of Chemistry (RSC)
dc.relation.ispartofseriesNanoscale Advances
dc.rightsCC BY-NC 4.0
dc.titleCovalent and non-covalent coupling of a Au102 nanocluster with a fluorophore : energy transfer, quenching and intracellular pH sensing
dc.typearticle
dc.identifier.urnURN:NBN:fi:jyu-202111085536
dc.contributor.laitosKemian laitosfi
dc.contributor.laitosBio- ja ympäristötieteiden laitosfi
dc.contributor.laitosFysiikan laitosfi
dc.contributor.laitosDepartment of Chemistryen
dc.contributor.laitosDepartment of Biological and Environmental Scienceen
dc.contributor.laitosDepartment of Physicsen
dc.contributor.oppiaineOrgaaninen kemiafi
dc.contributor.oppiaineFysikaalinen kemiafi
dc.contributor.oppiaineSolu- ja molekyylibiologiafi
dc.contributor.oppiaineNanoscience Centerfi
dc.contributor.oppiaineOrganic Chemistryen
dc.contributor.oppiainePhysical Chemistryen
dc.contributor.oppiaineCell and Molecular Biologyen
dc.contributor.oppiaineNanoscience Centeren
dc.type.urihttp://purl.org/eprint/type/JournalArticle
dc.description.reviewstatuspeerReviewed
dc.format.pagerange6649-6658
dc.relation.issn2516-0230
dc.relation.numberinseries23
dc.relation.volume3
dc.type.versionpublishedVersion
dc.rights.copyright© 2021 the Authors
dc.rights.accesslevelopenAccessfi
dc.relation.grantnumber292352
dc.relation.grantnumber290677
dc.relation.grantnumber294217
dc.subject.ysofluoresenssi
dc.subject.ysopH
dc.subject.ysonanohiukkaset
dc.subject.ysokemialliset sidokset
dc.subject.ysoväriaineet
dc.subject.ysoanturit
dc.format.contentfulltext
jyx.subject.urihttp://www.yso.fi/onto/yso/p3265
jyx.subject.urihttp://www.yso.fi/onto/yso/p4555
jyx.subject.urihttp://www.yso.fi/onto/yso/p23451
jyx.subject.urihttp://www.yso.fi/onto/yso/p10130
jyx.subject.urihttp://www.yso.fi/onto/yso/p2176
jyx.subject.urihttp://www.yso.fi/onto/yso/p11460
dc.rights.urlhttps://creativecommons.org/licenses/by-nc/4.0/
dc.relation.doi10.1039/D1NA00368B
dc.relation.funderSuomen Akatemiafi
dc.relation.funderSuomen Akatemiafi
dc.relation.funderSuomen Akatemiafi
dc.relation.funderAcademy of Finlanden
dc.relation.funderAcademy of Finlanden
dc.relation.funderAcademy of Finlanden
jyx.fundingprogramAkatemiaprofessorin tehtävä, SAfi
jyx.fundingprogramAkatemiahanke, SAfi
jyx.fundingprogramAkatemiaprofessorin tutkimuskulut, SAfi
jyx.fundingprogramResearch post as Academy Professor, AoFen
jyx.fundingprogramAcademy Project, AoFen
jyx.fundingprogramResearch costs of Academy Professor, AoFen
jyx.fundinginformationThis work was supported by Academy of Finland (Grants 292352, 294217, 290677, 257125 to H. H., G. G. and V. M.) and Jane and Aatos Erkko foundation (V. M.).


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record

CC BY-NC 4.0
Except where otherwise noted, this item's license is described as CC BY-NC 4.0