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dc.contributor.authorKunttu, Heidi M. T.
dc.contributor.authorRuntuvuori-Salmela, Anniina
dc.contributor.authorMiddelboe, Mathias
dc.contributor.authorClark, Jason
dc.contributor.authorSundberg, Lotta-Riina
dc.date.accessioned2021-11-03T07:10:53Z
dc.date.available2021-11-03T07:10:53Z
dc.date.issued2021
dc.identifier.citationKunttu, H. M. T., Runtuvuori-Salmela, A., Middelboe, M., Clark, J., & Sundberg, L.-R. (2021). Comparison of Delivery Methods in Phage Therapy against Flavobacterium columnare Infections in Rainbow Trout. <i>Antibiotics</i>, <i>10</i>(8), Article 914. <a href="https://doi.org/10.3390/antibiotics10080914" target="_blank">https://doi.org/10.3390/antibiotics10080914</a>
dc.identifier.otherCONVID_99135392
dc.identifier.urihttps://jyx.jyu.fi/handle/123456789/78465
dc.description.abstractViruses of bacteria, bacteriophages, specifically infect their bacterial hosts with minimal effects on the surrounding microbiota. They have the potential to be used in the prevention and treatment of bacterial infections, including in the field of food production. In aquaculture settings, disease-causing bacteria are often transmitted through the water body, providing several applications for phage-based targeting of pathogens, in the rearing environment, and in the fish. We tested delivery of phages by different methods (via baths, in phage-coated material, and via oral delivery in feed) to prevent and treat Flavobacterium columnare infections in rainbow trout fry using three phages (FCOV-S1, FCOV-F2, and FCL-2) and their hosts (FCO-S1, FCO-F2, and B185, respectively). Bath treatments given before bacterial infection and at the onset of the disease symptoms were the most efficient way to prevent F. columnare infections in rainbow trout, possibly due to the external nature of the disease. In a flow-through system, the presence of phage-coated plastic sheets delayed the onset of the disease. The oral administration of phages first increased disease progression, although total mortality was lower at the end of the experiment. When analysed for shelf-life, phage titers remained highest when maintained in bacterial culture media and in sterile lake water. Our results show that successful phage therapy treatment in the aquaculture setting requires optimisation of phage delivery methods in vivo.en
dc.format.mimetypeapplication/pdf
dc.language.isoeng
dc.publisherMDPI AG
dc.relation.ispartofseriesAntibiotics
dc.rightsCC BY 4.0
dc.subject.otheraquaculture
dc.subject.otherbacteriophage
dc.subject.otherbacterial infection
dc.subject.othercolumnaris disease
dc.subject.otherFlavobacterium columnare
dc.subject.otherphage therapy
dc.subject.otherphage delivery
dc.subject.otherrainbow trout
dc.subject.othertreatment
dc.subject.othervirulence
dc.titleComparison of Delivery Methods in Phage Therapy against Flavobacterium columnare Infections in Rainbow Trout
dc.typearticle
dc.identifier.urnURN:NBN:fi:jyu-202111035490
dc.contributor.laitosBio- ja ympäristötieteiden laitosfi
dc.contributor.laitosDepartment of Biological and Environmental Scienceen
dc.contributor.oppiaineAkvaattiset tieteetfi
dc.contributor.oppiaineSolu- ja molekyylibiologiafi
dc.contributor.oppiaineNanoscience Centerfi
dc.contributor.oppiaineAquatic Sciencesen
dc.contributor.oppiaineCell and Molecular Biologyen
dc.contributor.oppiaineNanoscience Centeren
dc.type.urihttp://purl.org/eprint/type/JournalArticle
dc.description.reviewstatuspeerReviewed
dc.relation.issn2079-6382
dc.relation.numberinseries8
dc.relation.volume10
dc.type.versionpublishedVersion
dc.rights.copyright© 2021 by the authors. Licensee MDPI, Basel, Switzerland.
dc.rights.accesslevelopenAccessfi
dc.relation.grantnumber314939
dc.relation.grantnumber
dc.relation.grantnumber311962
dc.subject.ysofagiterapia
dc.subject.ysolohikalat
dc.subject.ysovesiviljely (kalatalous)
dc.subject.ysohoitomenetelmät
dc.subject.ysobakteeritaudit
dc.subject.ysobakteriofagit
dc.subject.ysobakteerit
dc.format.contentfulltext
jyx.subject.urihttp://www.yso.fi/onto/yso/p29496
jyx.subject.urihttp://www.yso.fi/onto/yso/p4245
jyx.subject.urihttp://www.yso.fi/onto/yso/p5099
jyx.subject.urihttp://www.yso.fi/onto/yso/p392
jyx.subject.urihttp://www.yso.fi/onto/yso/p19293
jyx.subject.urihttp://www.yso.fi/onto/yso/p25303
jyx.subject.urihttp://www.yso.fi/onto/yso/p1749
dc.rights.urlhttps://creativecommons.org/licenses/by/4.0/
dc.relation.datasethttps://doi.org/10.17011/jyx/dataset/77208
dc.relation.doi10.3390/antibiotics10080914
dc.relation.funderSuomen Akatemiafi
dc.relation.funderJane ja Aatos Erkon säätiöfi
dc.relation.funderSuomen Akatemiafi
dc.relation.funderAcademy of Finlanden
dc.relation.funderJane and Aatos Erkko Foundationen
dc.relation.funderAcademy of Finlanden
jyx.fundingprogramAkatemiahanke, SAfi
jyx.fundingprogramSäätiöfi
jyx.fundingprogramMuut, SAfi
jyx.fundingprogramAcademy Project, AoFen
jyx.fundingprogramFoundationen
jyx.fundingprogramOthers, AoFen
jyx.fundinginformationThis work resulted from the BONUS FLAVOPHAGE project supported by BONUS (Art 185), funded jointly by the EU and Academy of Finland. We also acknowledge funding from the Academy of Finland (grant #314939) and Jane and Aatos Erkko Foundation.
datacite.isSupplementedBy.doi10.17011/jyx/dataset/77742
datacite.isSupplementedByKunttu, Heidi; Runtuvuori-Salmela, Anniina; Middelboe, Mathias; Clark, Jason; Sundberg, Lotta-Riina (2021). Comparison of Delivery Methods in Phage Therapy against Flavobacterium columnare Infections in Rainbow Trout. V. 26.7.2021. https://doi.org/10.17011/jyx/dataset/77742. <a href="http://urn.fi/URN:NBN:fi:jyu-202109104846">https://urn.fi/URN:NBN:fi:jyu-202109104846</a>


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