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dc.contributor.authorShao, Shuai
dc.contributor.authorDeng, Sha
dc.contributor.authorJiang, Qingyun
dc.contributor.authorZhang, Hangyu
dc.contributor.authorZhang, Zhengyao
dc.contributor.authorLi, Na
dc.contributor.authorCong, Fengyu
dc.contributor.authorTiihonen, Timo
dc.contributor.authorLiu, Bo
dc.date.accessioned2021-10-15T08:17:23Z
dc.date.available2021-10-15T08:17:23Z
dc.date.issued2021
dc.identifier.citationShao, S., Deng, S., Jiang, Q., Zhang, H., Zhang, Z., Li, N., Cong, F., Tiihonen, T., & Liu, B. (2021). A DNA‐Encoded FRET Biosensor for Visualizing the Tension across Paxillin in Living Cells upon Shear Stress. <i>Analysis and Sensing</i>, <i>2</i>(1), -. <a href="https://doi.org/10.1002/anse.202100033" target="_blank">https://doi.org/10.1002/anse.202100033</a>
dc.identifier.otherCONVID_101470646
dc.identifier.urihttps://jyx.jyu.fi/handle/123456789/78225
dc.description.abstractPaxillin is a potential participant in the direct intracellular force transmission which is considered as the foundation of cells sensing and responding to extracellular environment. However, the detection of tension across paxillin has not been achieved due to lacking microsized tools. Herein, a paxillin tension sensor (PaxTs) based on Fluorescence Resonance Energy Transfer (FRET) technique was constructed. PaxTs can be expressed and assembled to FA sites spontaneously to visualize the tension across paxillin with FRET efficiency of ~62.4% in living cells. The tension across paxillin was found to decrease upon shear stress, in which the membrane fluidity and contractility of actin acted as cushions. It is observed that paxillin participates in the pathway of cell membrane-cytoskeleton-FAs for force transmission upon mechanical force in real time visualization, which provides a promising new method to investigate the direct intracellular force transmission in biology and technology.en
dc.format.mimetypeapplication/pdf
dc.language.isoeng
dc.publisherWiley
dc.relation.ispartofseriesAnalysis and Sensing
dc.rightsIn Copyright
dc.subject.otherbiosensors
dc.subject.otherfocal adhesions
dc.subject.otherFRET
dc.subject.otherpaxillin
dc.subject.othershear stress
dc.titleA DNA‐Encoded FRET Biosensor for Visualizing the Tension across Paxillin in Living Cells upon Shear Stress
dc.typearticle
dc.identifier.urnURN:NBN:fi:jyu-202110155257
dc.contributor.laitosInformaatioteknologian tiedekuntafi
dc.contributor.laitosFaculty of Information Technologyen
dc.contributor.oppiaineSecure Communications Engineering and Signal Processingfi
dc.contributor.oppiaineOhjelmisto- ja tietoliikennetekniikkafi
dc.contributor.oppiaineLaskennallinen tiedefi
dc.contributor.oppiaineTietotekniikkafi
dc.contributor.oppiaineSecure Communications Engineering and Signal Processingen
dc.contributor.oppiaineSoftware and Communications Engineeringen
dc.contributor.oppiaineComputational Scienceen
dc.contributor.oppiaineMathematical Information Technologyen
dc.type.urihttp://purl.org/eprint/type/JournalArticle
dc.description.reviewstatuspeerReviewed
dc.format.pagerange-
dc.relation.issn2629-2742
dc.relation.numberinseries1
dc.relation.volume2
dc.type.versionacceptedVersion
dc.rights.copyright© 2021 Wiley-VCH GmbH
dc.rights.accesslevelopenAccessfi
dc.subject.ysosolut
dc.subject.ysomallintaminen
dc.subject.ysobiosensorit
dc.subject.ysovoimansiirto
dc.format.contentfulltext
jyx.subject.urihttp://www.yso.fi/onto/yso/p2409
jyx.subject.urihttp://www.yso.fi/onto/yso/p3533
jyx.subject.urihttp://www.yso.fi/onto/yso/p22008
jyx.subject.urihttp://www.yso.fi/onto/yso/p5699
dc.rights.urlhttp://rightsstatements.org/page/InC/1.0/?language=en
dc.relation.doi10.1002/anse.202100033
jyx.fundinginformationThis work was supported by National Natural Science Foundation of China (Grant No. 32071252), and the Fundamental Research Funds for the Central Universities [DUT20LAB119] in Dalian University of Technology in China.


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