FRET biosensor allows spatio-temporal observation of shear stress-induced polar RhoGDIα activation
Shao, S., Liao, X., Xie, F., Deng, S., Liu, X., Ristaniemi, T., & Liu, B. (2018). FRET biosensor allows spatio-temporal observation of shear stress-induced polar RhoGDIα activation. Communications Biology, 1, Article 224. https://doi.org/10.1038/s42003-018-0232-2
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2018Copyright
© The Author(s) 2018
Rho GDP-dissociation inhibitor α (RhoGDIα) is a known negative regulator of the Rho family
that shuts off GDP/GTP cycling and cytoplasm/membrane translocation to regulate cell
migration. However, to our knowledge, no reports are available that focus on how the
RhoGDIα-Rho GTPases complex is activated by laminar flow through exploring the activation
of RhoGDIα itself. Here, we constructed a new biosensor using fluorescence resonance
energy transfer (FRET) technology to measure the spatio-temporal activation of RhoGDIα in
its binding with Rho GTPases in living HeLa cells. Using this biosensor, we find that the
dissociation of the RhoGDIα-Rho GTPases complex is increased by shear stress, and its
dissociation rate varies with subcellular location. Moreover, this process is mediated by
membrane fluidity, cytoskeleton and Src activity, which indicates that the regulation of
RhoGDIα activation under shear stress application represents a relatively separate pathway
from the shear stress-induced Rho pathway.
...
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Nature Publishing GroupISSN Search the Publication Forum
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