The discovery, distribution, and diversity of DNA viruses associated with Drosophila melanogaster in Europe
Wallace, M. A., Coffman, K. A., Gilbert, C., Ravindran, S., Albery, G. F., Abbott, J., Argyridou, E., Bellosta, P., Betancourt, A. J., Colinet, H., Eric, K., Glaser-Schmitt, A., Grath, S., Jelic, M., Kankare, M., Kozeretska, I., Loeschcke, V., Montchamp-Moreau, C., Ometto, L., . . . Obbard, D. J. (2021). The discovery, distribution, and diversity of DNA viruses associated with Drosophila melanogaster in Europe. Virus Evolution, 7(1), Article veab031. https://doi.org/10.1093/ve/veab031
Published inVirus Evolution
© 2021 the Authors
Drosophila melanogaster is an important model for antiviral immunity in arthropods, but very few DNA viruses have been described from the family Drosophilidae. This deficiency limits our opportunity to use natural host-pathogen combinations in experimental studies, and may bias our understanding of the Drosophila virome. Here we report fourteen DNA viruses detected in a metagenomic analysis of approximately 6500 pool-sequenced Drosophila, sampled from 47 European locations between 2014 and 2016. These include three new nudiviruses, a new and divergent entomopoxvirus, a virus related to Leptopilina boulardi filamentous virus, and a virus related to Musca domestica salivary gland hypertrophy virus. We also find an endogenous genomic copy of galbut virus, a dsRNA partitivirus, segregating at very low frequency. Remarkably, we find that Drosophila Vesanto virus, a small DNA virus previously described as a bidnavirus, may be composed of up to 12 segments and thus represent a new lineage of segmented DNA viruses. Two of the DNA viruses, Drosophila Kallithea nudivirus and Drosophila Vesanto virus are relatively common, found in 2% or more of wild flies. The others are rare, with many likely to be represented by a single infected fly. We find that virus prevalence in Europe reflects the prevalence seen in publicly-available datasets, with Drosophila Kallithea nudivirus and Drosophila Vesanto virus the only ones commonly detectable in public data from wild-caught flies and large population cages, and the other viruses being rare or absent. These analyses suggest that DNA viruses are at lower prevalence than RNA viruses in D. melanogaster, and may be less likely to persist in laboratory cultures. Our findings go some way to redressing an earlier bias toward RNA virus studies in Drosophila, and lay the foundation needed to harness the power of Drosophila as a model system for the study of DNA viruses. ...
PublisherOxford University Press (OUP)
Publication in research information system
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Related funder(s)Academy of Finland
Funding program(s)Research post as Academy Research Fellow, AoF; Academy Project, AoF
Additional information about fundingMegan Wallace was supported by the UK Natural Environmental Research Council through the E3 doctoral training programme (NE/L002558/1), and Sanjana Ravindran was supported by Wellcome Trust PhD programme (108905/Z/15/Z). Andrea Betancourt received funding from BBSRC grant BB/P00685X/1 Thomas Flatt received funding from Swiss National Science Foundation grants 31003A182262, PP00P3_165836, and PP00P3_133641/1. Clément Gilbert received funding from Agence Nationale de la Recherche (grant ANR-15- CE32-0011-01) Josefa González received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (H2020-ERC-2014- CoG-647900) and from the Fundación Española para la Ciencia y la Tecnologia-Ministerio de Economía y Competitividad (FCT-15-10187). Sonja Grath received funding from Deutsche Forschungsgemeinschaft grant GR 4495/2 Maaria Kankare received funding from Academy of Finland projects 268214 and 322980. Martin Kapun received funding from Austrian Science Fund (FWF) grant P32275. Volker Loeschcke received funding from Danish Research council for natural Sciences (FNU) grant nr 4002-00113B Banu Sebnem Onder received funding from the Scientific and Technological Research Council of Turkey (TUBITAK) (Grant No. 214Z238) John Parsch received funding from Deutsche Forschungsgemeinschaft grant PA 903/8 Marina Stamenkovic-Radak, Marija Savic Veselinovic, and Mihailo Jelic received funding from the Ministry of Education, Science and Technological Development of the Republic of Serbia (Grant number 451-03-68/2020-14/200178) Fabian Staubach received funding from Deutsche Forschungsgemeinschaft grant STA1154/4-1; Projektnummer 408908608 Marija Tanaskovic, Aleksandra Patenkovic, and Katarina Eric received funding from the Ministry of Education, Science and Technological Development of the Republic of Serbia (Grant number 451-03-68/2020-14/200007). The DrosEU consortium has been funded by a Special Topics Network (STN) grant by the European Society of Evolutionary Biology (ESEB). ...
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