Photoactivation of Drosophila melanogaster cryptochrome through sequential conformational transitions
Berntsson, O., Rodriguez, R., Henry, L., Panman, M. R., Hughes, A. J., Einholz, C., Weber, S., Ihalainen, J. A., Henning, R., Kosheleva, I., Schleicher, E., & Westenhoff, S. (2019). Photoactivation of Drosophila melanogaster cryptochrome through sequential conformational transitions. Science Advances, 5(7), Article eaaw1531. https://doi.org/10.1126/sciadv.aaw1531
Published inScience Advances
© 2019 The Authors
Cryptochromes are blue-light photoreceptor proteins, which provide input to circadian clocks. The crypto- chrome from Drosophila melanogaster (DmCry) modulates the degradation of Timeless and itself. It is unclear how light absorption by the chromophore and the subsequent redox reactions trigger these events. Here, we use nano- to millisecond time-resolved x-ray solution scattering to reveal the light-activated conformational changes in DmCry and the related (6-4) photolyase. DmCry undergoes a series of structural changes, culminat- ing in the release of the carboxyl-terminal tail (CTT). The photolyase has a simpler structural response. We find that the CTT release in DmCry depends on pH. Mutation of a conserved histidine, important for the biochemical activity of DmCry, does not affect transduction of the structural signal to the CTT. Instead, molecular dynamics simulations suggest that it stabilizes the CTT in the resting-state conformation. Our structural photocycle un- ravels the first molecular events of signal transduction in an animal cryptochrome. ...
PublisherAmerican Association for the Advancement of Science
Publication in research information system
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Related funder(s)Academy of Finland
Funding program(s)Academy Project, AoF
Additional information about fundingS.Wes. acknowledges funding from the Swedish Foundation for International Cooperation in Research and Higher Education, the European Research Council (725642), and the Foundation of Strategic Research, Sweden. E.S. and S.Web. thank the DFG (235777276/GRK1976) for financial support. J.A.I. acknowledges a grant from the Academy of Finland (296135) and the Jane and Aatos Erkko Foundation.
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