DNA recovery from Droplet Digital™ PCR emulsions using liquid nitrogen
Dutra, L., Franz, O., Puupponen, V.-M., & Tiirola, M. (2020). DNA recovery from Droplet Digital™ PCR emulsions using liquid nitrogen. Biotechniques, 69(9), 451-454. https://doi.org/10.2144/btn-2020-0076
© Authors, 2020
Droplet microfluidics is a technology that enables the production and manipulation of small volumes. In biosciences, the most popular application of this technology is Droplet Digital™ PCR (ddPCR™), where parallel nanoliter-scale PCR assays are used to provide a high sensitivity and specificity for DNA detection. However, the recovery of PCR products for downstream applications such as sequencing can be challenging due to the droplets' stability. Here we compared five methods for disrupting the droplets to recover DNA. We found that rapid freezing in liquid nitrogen results in a clear phase separation and recovery of up to 70% of the DNA content. Liquid nitrogen freezing can thus offer a simple and environmentally friendly protocol for recovering DNA from ddPCR.
PublisherFuture Science Ltd
ISSN Search the Publication Forum0736-6205
Publication in research information system
MetadataShow full item record
Related funder(s)Academy of Finland; European Commission
Funding program(s)Academy Project, AoF; FP7 (EU's 7th Framework Programme)
The content of the publication reflects only the author’s view. The funder is not responsible for any use that may be made of the information it contains.
Additional information about fundingThis work was funded by the Academy of Finland (grant 323063) and European Research Council CoG grant (615146) and PoC grant (812729; EU FP7 and H2020 programs, respectively) to M Tiirola.
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