Näytä suppeat kuvailutiedot

dc.contributor.authorRigaud, Cyril
dc.contributor.authorEriksson, Andreas
dc.contributor.authorKrasnov, Aleksei
dc.contributor.authorWincent, Emma
dc.contributor.authorPakkanen, Hannu
dc.contributor.authorLehtivuori, Heli
dc.contributor.authorIhalainen, Janne
dc.contributor.authorVehniäinen, Eeva-Riikka
dc.date.accessioned2020-07-31T05:38:29Z
dc.date.available2020-07-31T05:38:29Z
dc.date.issued2020
dc.identifier.citationRigaud, Cyril; Eriksson, Andreas; Krasnov, Aleksei; Wincent, Emma; Pakkanen, Hannu; Lehtivuori, Heli; Ihalainen, Janne; Vehniäinen, Eeva-Riikka (2020). Retene, pyrene and phenanthrene cause distinct molecular-level changes in the cardiac tissue of rainbow trout (Oncorhynchus mykiss) larvae, part 1 – Transcriptomics. Science of the Total Environment, 745, 141031. DOI: 10.1016/j.scitotenv.2020.141031
dc.identifier.otherCONVID_41654444
dc.identifier.urihttps://jyx.jyu.fi/handle/123456789/71299
dc.description.abstractPolycyclic aromatic hydrocarbons (PAHs) are contaminants of concern that impact every sphere of the environment. Despite several decades of research, their mechanisms of toxicity are still poorly understood. This study explores the mechanisms of cardiotoxicity of the three widespread model PAHs retene, pyrene and phenanthrene in the rainbow trout (Oncorhynchus mykiss) early life stages. Newly hatched larvae were exposed to each individual compound at sublethal doses causing no significant increase in the prevalence of deformities. Changes in the cardiac transcriptome were assessed after 1, 3, 7 and 14 days of exposure using custom Salmo salar microarrays. The highest number of differentially expressed genes was observed after 1 or 3 days of exposure, and retene was the most potent compound in that regard. Over-representation analyses suggested that genes related to cardiac ion channels, calcium homeostasis and muscle contraction (actin binding, troponin and myosin complexes) were especially targeted by retene. Pyrene was also able to alter similar myosin-related genes, but at a different timing and in an opposite direction, suggesting compound-specific mechanisms of toxicity. Pyrene and to a lesser extent phenanthrene were altering key genes linked to the respiratory electron transport chain and to oxygen and iron metabolism. Overall, phenanthrene was not very potent in inducing changes in the cardiac transcriptome despite being apparently metabolized at a slower rate than retene and pyrene. The present study shows that exposure to different PAHs during the first few days of the swim-up stage can alter the expression of key genes involved into the cardiac development and function, which could potentially affect negatively the fitness of the larvae in the long term.en
dc.format.mimetypeapplication/pdf
dc.languageeng
dc.publisherElsevier BV
dc.relation.ispartofseriesScience of the Total Environment
dc.rightsCC BY-NC-ND 4.0
dc.subject.otheraquatic toxicology
dc.subject.othercardiotoxicity
dc.subject.otherpolycyclic aromatic hydrocarbons (PAHs)
dc.subject.othertranscriptomics
dc.titleRetene, pyrene and phenanthrene cause distinct molecular-level changes in the cardiac tissue of rainbow trout (Oncorhynchus mykiss) larvae, part 1 – Transcriptomics
dc.typearticle
dc.identifier.urnURN:NBN:fi:jyu-202007315447
dc.contributor.laitosFysiikan laitosfi
dc.contributor.laitosBio- ja ympäristötieteiden laitosfi
dc.contributor.laitosDepartment of Physicsen
dc.contributor.laitosDepartment of Biological and Environmental Scienceen
dc.type.urihttp://purl.org/eprint/type/JournalArticle
dc.description.reviewstatuspeerReviewed
dc.format.pagerange141031
dc.relation.issn0048-9697
dc.relation.volume745
dc.type.versionacceptedVersion
dc.rights.copyright© 2020 Elsevier B.V. All rights reserved.
dc.rights.accesslevelembargoedAccessfi
dc.relation.grantnumber294066
dc.relation.grantnumber285296
dc.relation.grantnumber319284
dc.subject.ysoekotoksikologia
dc.subject.ysoaromaattiset hiilivedyt
dc.subject.ysolohikalat
dc.subject.ysoepäpuhtaudet
dc.subject.ysokirjolohi
dc.subject.ysotoksiinit
dc.subject.ysovesistöt
dc.subject.ysobiologiset vaikutukset
dc.subject.ysomyrkyllisyys
dc.format.contentfulltext
jyx.subject.urihttp://www.yso.fi/onto/yso/p19671
jyx.subject.urihttp://www.yso.fi/onto/yso/p23503
jyx.subject.urihttp://www.yso.fi/onto/yso/p4245
jyx.subject.urihttp://www.yso.fi/onto/yso/p433
jyx.subject.urihttp://www.yso.fi/onto/yso/p12317
jyx.subject.urihttp://www.yso.fi/onto/yso/p10863
jyx.subject.urihttp://www.yso.fi/onto/yso/p1157
jyx.subject.urihttp://www.yso.fi/onto/yso/p7702
jyx.subject.urihttp://www.yso.fi/onto/yso/p12637
dc.rights.urlhttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.relation.doi10.1016/j.scitotenv.2020.141031
dc.relation.funderSuomen Akatemiafi
dc.relation.funderSuomen Akatemiafi
dc.relation.funderSuomen Akatemiafi
dc.relation.funderAcademy of Finlanden
dc.relation.funderAcademy of Finlanden
dc.relation.funderAcademy of Finlanden
jyx.fundingprogramAkatemiatutkijan tutkimuskulut, SAfi
jyx.fundingprogramAkatemiatutkijan tehtävä, SAfi
jyx.fundingprogramAkatemiatutkijan tutkimuskulut, SAfi
jyx.fundingprogramResearch costs of Academy Research Fellow, AoFen
jyx.fundingprogramResearch post as Academy Research Fellow, AoFen
jyx.fundingprogramResearch costs of Academy Research Fellow, AoFen
jyx.fundinginformationThe present study was funded by the Academy of Finland (projects 285296, 294066 and 319284 to Eeva -Riikka Vehniäinen). The authors are grateful to Mervi Koistinen, Terhi Rahkonen, Jaakko Litmanen as well as the personnel of the Konnevesi Research Station for their technical assistance. We also thank Gerrit Timmerhaus for his precious help during the preparation of the microarrays.


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