Tissue-Specific Dynamics in the Endophytic Bacterial Communities in Arctic Pioneer Plant Oxyria digyna
Given, C., Häikiö, E., Kumar, M., & Nissinen, R. (2020). Tissue-Specific Dynamics in the Endophytic Bacterial Communities in Arctic Pioneer Plant Oxyria digyna. Frontiers in Plant Science, 11, Article 561. https://doi.org/10.3389/fpls.2020.00561
Published inFrontiers in Plant Science
© 2020 the Author(s)
The rapid developments in the next-generation sequencing methods in the recent years have provided a wealth of information on the community structures and functions of endophytic bacteria. However, the assembly processes of these communities in different plant tissues are still currently poorly understood, especially in wild plants in natural settings. The aim of this study was to compare the composition of endophytic bacterial communities in leaves and roots of arcto-alpine pioneer plant Oxyria digyna, and investigate, how plant tissue (leaf or root) or plant origin affect the community assembly. To address this, we planted micropropagated O. digyna plants with low bacterial load (bait plants) in experimental site with native O. digyna population, in the Low Arctic. The endophytic bacterial community structures in the leaves and roots of the bait plants were analyzed after one growing season and one year in the field, and compared to those of the wild plants growing at the same site. 16S rRNA gene targeted sequencing revealed that endophytic communities in the roots were more diverse than in the leaves, and the diversity in the bait plants increased in the field, and was highest in the wild plants. Both tissue type and plant group had strong impact on the endophytic bacterial community structures. Firmicutes were highly abundant in the leaf communities of both plant types. Proteobacteria and Bacteroidetes were more abundant in the roots, albeit with different relative abundances in different plant groups. The community structures in the bait plants changed in the field over time, and increasingly resembled the wild plant endophytic communities. This was due to the changes in the relative abundances of several bacterial taxa, as well as species acquisition in the field, but with no species turnover. Several OTUs that were acquired by the bait plants in the field and represent phosphate solubilizing and diazotrophic bacterial taxa, suggesting major role in nutrient acquisition of these bacteria for this nonmycorrhizal plant, thriving in the nutrient poor arctic soils. ...
Publication in research information system
MetadataShow full item record
Related funder(s)Academy of Finland
Funding program(s)Research post as Academy Research Fellow, AoF
Additional information about fundingThis work was funded by the Academy of Finland (grant #259180 for RN).
Showing items with similar title or keywords.
Strong Regionality and Dominance of Anaerobic Bacterial Taxa Characterize Diazotrophic Bacterial Communities of the Arcto-Alpine Plant Species Oxyria digyna and Saxifraga oppositifolia Gopala Krishnan, Manoj Kumar; van Elsas, Jan Dirk; Nissinen, Riitta (Frontiers Research Foundation, 2017)Arctic and alpine biomes are most often strongly nitrogen-limited, and hence biological nitrogen fixation is a strong driver of these ecosystems. Both biomes are characterized by low temperatures and short growing seasons, ...
Assembly and functioning of endophytic bacterial communities in arcto-alpine pioneer plant Oxyria digyna Given, Cindy Jittrapan (2019)Plant microbiomes consist of diverse communities of microorganisms, among which bacteria are highly abundant. The microbiomes are crucial for plants as they rely on their microbial associates for many essential functions. ...
Bacterial Diversity in a Dynamic and Extreme Sub-Arctic Watercourse (Pasvik River, Norwegian Arctic) Papale, Maria; Rappazzo, Alessandro Ciro; Mikkonen, Anu; Rizzo, Carmen; Moscheo, Federica; Conte, Antonella; Michaud, Luigi; Lo Giudice, Angelina (MDPI AG, 2020)Microbial communities promptly respond to the environmental perturbations, especially in the Arctic and sub-Arctic systems that are highly impacted by climate change, and fluctuations in the diversity level of microbial ...
Plants Assemble Species Specific Bacterial Communities From Common Core Taxa in Three Arcto-Alpine Climate Zones Gopala Krishnan, Manoj Kumar; Brader, Günter; Sessitsch, Angela; Mäki, Anita; Elsas, Jan Dirk Van; Nissinen, Riitta (Frontiers Research Foundation, 2017)Evidence for the pivotal role of plant-associated bacteria to plant health and productivity has accumulated rapidly in the last years. However, key questions related to what drives plant bacteriomes remain unanswered, ...
Poosakkannu, Anbu (University of Jyväskylä, 2016)Plant-associated microbes could play a role in plant colonization of sand dune ecosystems, but microbes associated with plants colonizing those ecosystems in the arctic are poorly known. I characterized Deschampsia ...