Assembly and functioning of endophytic bacterial communities in arcto-alpine pioneer plant Oxyria digyna
Julkaistu sarjassa
JYU dissertationsTekijät
Päivämäärä
2019Tekijänoikeudet
© The Author & University of Jyväskylä
Plant microbiomes consist of diverse communities of microorganisms, among
which bacteria are highly abundant. The microbiomes are crucial for plants as
they rely on their microbial associates for many essential functions. The goal of
this thesis was to study the functional diversity and assembly rules of
endophytic bacterial communities in different plant tissues of the arcto-alpine
pioneer plant species, Oxyria digyna. I used high-throughput sequencing and
bacterial isolations to characterize the endophytic communities in the leaves
and roots of native O. digyna plants (wild plants) and micropropagated aseptic
plants (bait plants) in the field. Wild plants and tissue-propagated bait plants
were shown to harbor endophytic communities with taxonomically similar
structures, but with divergent functional profiles. Several plant-associated
microbial traits, including nitrogen fixation and phosphate solubilization,
correlated with the plant type, as did also the temperature optima of the
endophytic isolates. To study endophyte community assembly, I inoculated bait
plants via either leaves or roots with bacterial consortia specific for leaves or
roots of O. digyna. The assembly of endophytic communities in different tissues
was primarily limited by the adaptation to plant niche in the leaves, and
colonization ability and competitiveness in the roots. Plant inoculation with
bacterial consortia originating from different tissues (leaves or roots)
restructured the innate endophytic communities, and had divergent impact on
the plant phenotype. The observed differences in the plant phenotype and
fitness could be explained by direct impact of inoculated bacteria on the plant
metabolism (plant-microbe interaction) or indirect impact via altered
functioning of the innate endophyte community (microbe-microbe interactions).
Taken together, the findings in this thesis demonstrate that the endophytic
bacterial communities are tissue-specific and tightly associated with their host
plant, but at the same time, are highly dynamic, rapidly adapting to changes in
environmental conditions.
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Julkaisija
Jyväskylän yliopistoISBN
978-951-39-7690-3ISSN Hae Julkaisufoorumista
2489-9003Julkaisuun sisältyy osajulkaisuja
- Artikkeli I: Given, Cindy; Häikiö, Elina; Kumar, Manoj; Nissinen, Riitta (2020). Tissue-Specific Dynamics in the Endophytic Bacterial Communities in Arctic Pioneer Plant Oxyria digyna. Frontiers in Plant Science, 11, 561. DOI: 10.3389/fpls.2020.00561
- Artikkeli II: Given C.J., Häikiö E. & Nissinen R. (2018) The functional diversity of tissue- and plant type-specific endophytic bacterial community of arctic plant, Oxyria digyna. Manuscript.
- Artikkeli III: Given C.J., Häikiö E. & Nissinen R. (2018) The factors determining the assembly of tissue-specific endophytic bacterial communities in Oxyria digyna. Manuscript.
- Artikkeli IV: Given C.J. & Nissinen R. (2018) Inoculation with endophytic bacteria confers heat stress tolerance to Oxyria digyna. Manuscript.
Asiasanat
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