Näytä suppeat kuvailutiedot

dc.contributor.authorRuokolainen, Visa
dc.contributor.authorLaajala, Mira
dc.contributor.authorMarjomäki, Varpu
dc.date.accessioned2020-04-28T07:01:06Z
dc.date.available2020-04-28T07:01:06Z
dc.date.issued2020
dc.identifier.citationRuokolainen, V., Laajala, M., & Marjomäki, V. (2020). Real-time Fluorescence Measurement of Enterovirus Uncoating. <i>Bio-protocol</i>, <i>10</i>(7), Article e3582. <a href="https://doi.org/10.21769/BioProtoc.3582" target="_blank">https://doi.org/10.21769/BioProtoc.3582</a>
dc.identifier.otherCONVID_35296445
dc.identifier.urihttps://jyx.jyu.fi/handle/123456789/68728
dc.description.abstractViruses need to open, i.e., uncoat, in order to release their genomes for efficient replication and translation. Especially for non-enveloped viruses, such as enteroviruses, the cues leading to uncoating are less well known. The status of the virus has previously been observed mainly by transmission electron microscopy using negative staining, cryo electron microscopy, X-ray crystallography or gradient separation (reviewed in Tuthill et al., 2010, Myllynen et al., 2016, Ruokolainen et al., 2019). However, monitoring of uncoating has been limited by the lack of methods detecting dynamic changes of the virions. Here, we present a real-time fluorescence based protocol, which detects the viral genome (RNA) during various stages of uncoating in vitro, while RNA is still inside the particle that has been expanded before the actual RNA release, and when the RNA has been totally released from the viral particle. Our method allows to explore how various molecular factors may promote or inhibit virus uncoating.en
dc.format.mimetypeapplication/pdf
dc.languageeng
dc.language.isoeng
dc.publisherBio-protocol
dc.relation.ispartofseriesBio-protocol
dc.rightsCC BY 4.0
dc.subject.otherPicornavirus
dc.subject.otherEnterovirus
dc.subject.otheruncoating
dc.subject.othergenome
dc.subject.otherRNA
dc.subject.otherRNase
dc.subject.otherSYBR Green II
dc.subject.otherfluorescence spectroscopy
dc.titleReal-time Fluorescence Measurement of Enterovirus Uncoating
dc.typearticle
dc.identifier.urnURN:NBN:fi:jyu-202004282931
dc.contributor.laitosBio- ja ympäristötieteiden laitosfi
dc.contributor.laitosDepartment of Biological and Environmental Scienceen
dc.contributor.oppiaineNanoscience Centerfi
dc.contributor.oppiaineSolu- ja molekyylibiologiafi
dc.contributor.oppiaineNanoscience Centeren
dc.contributor.oppiaineCell and Molecular Biologyen
dc.type.urihttp://purl.org/eprint/type/JournalArticle
dc.type.coarhttp://purl.org/coar/resource_type/c_2df8fbb1
dc.description.reviewstatuspeerReviewed
dc.relation.issn2331-8325
dc.relation.numberinseries7
dc.relation.volume10
dc.type.versionpublishedVersion
dc.rights.copyright©Authors, 2020
dc.rights.accesslevelopenAccessfi
dc.subject.ysoenterovirukset
dc.subject.ysospektroskopia
dc.subject.ysofluoresenssi
dc.subject.ysotutkimusmenetelmät
dc.subject.ysoinfektiot
dc.subject.ysoRNA
dc.format.contentfulltext
jyx.subject.urihttp://www.yso.fi/onto/yso/p20689
jyx.subject.urihttp://www.yso.fi/onto/yso/p10176
jyx.subject.urihttp://www.yso.fi/onto/yso/p3265
jyx.subject.urihttp://www.yso.fi/onto/yso/p415
jyx.subject.urihttp://www.yso.fi/onto/yso/p7316
jyx.subject.urihttp://www.yso.fi/onto/yso/p7689
dc.rights.urlhttps://creativecommons.org/licenses/by/4.0/
dc.relation.doi10.21769/BioProtoc.3582
jyx.fundinginformationWe acknowledge Jane and Aatos Erkko foundation and doctoral school of University of Jyväskylä for funding and the authors of the original paper (Ruokolainen et al., 2019) for contributing to the work where the protocol was introduced.
dc.type.okmA1


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