dc.contributor.author | Ruokolainen, Visa | |
dc.contributor.author | Laajala, Mira | |
dc.contributor.author | Marjomäki, Varpu | |
dc.date.accessioned | 2020-04-28T07:01:06Z | |
dc.date.available | 2020-04-28T07:01:06Z | |
dc.date.issued | 2020 | |
dc.identifier.citation | Ruokolainen, V., Laajala, M., & Marjomäki, V. (2020). Real-time Fluorescence Measurement of Enterovirus Uncoating. <i>Bio-protocol</i>, <i>10</i>(7), Article e3582. <a href="https://doi.org/10.21769/BioProtoc.3582" target="_blank">https://doi.org/10.21769/BioProtoc.3582</a> | |
dc.identifier.other | CONVID_35296445 | |
dc.identifier.uri | https://jyx.jyu.fi/handle/123456789/68728 | |
dc.description.abstract | Viruses need to open, i.e., uncoat, in order to release their genomes for efficient replication
and translation. Especially for non-enveloped viruses, such as enteroviruses, the cues leading to
uncoating are less well known. The status of the virus has previously been observed mainly by
transmission electron microscopy using negative staining, cryo electron microscopy, X-ray
crystallography or gradient separation (reviewed in Tuthill et al., 2010, Myllynen et al., 2016,
Ruokolainen et al., 2019). However, monitoring of uncoating has been limited by the lack of methods
detecting dynamic changes of the virions. Here, we present a real-time fluorescence based protocol,
which detects the viral genome (RNA) during various stages of uncoating in vitro, while RNA is still inside
the particle that has been expanded before the actual RNA release, and when the RNA has been totally
released from the viral particle. Our method allows to explore how various molecular factors may
promote or inhibit virus uncoating. | en |
dc.format.mimetype | application/pdf | |
dc.language | eng | |
dc.language.iso | eng | |
dc.publisher | Bio-protocol | |
dc.relation.ispartofseries | Bio-protocol | |
dc.rights | CC BY 4.0 | |
dc.subject.other | Picornavirus | |
dc.subject.other | Enterovirus | |
dc.subject.other | uncoating | |
dc.subject.other | genome | |
dc.subject.other | RNA | |
dc.subject.other | RNase | |
dc.subject.other | SYBR Green II | |
dc.subject.other | fluorescence spectroscopy | |
dc.title | Real-time Fluorescence Measurement of Enterovirus Uncoating | |
dc.type | article | |
dc.identifier.urn | URN:NBN:fi:jyu-202004282931 | |
dc.contributor.laitos | Bio- ja ympäristötieteiden laitos | fi |
dc.contributor.laitos | Department of Biological and Environmental Science | en |
dc.contributor.oppiaine | Nanoscience Center | fi |
dc.contributor.oppiaine | Solu- ja molekyylibiologia | fi |
dc.contributor.oppiaine | Nanoscience Center | en |
dc.contributor.oppiaine | Cell and Molecular Biology | en |
dc.type.uri | http://purl.org/eprint/type/JournalArticle | |
dc.type.coar | http://purl.org/coar/resource_type/c_2df8fbb1 | |
dc.description.reviewstatus | peerReviewed | |
dc.relation.issn | 2331-8325 | |
dc.relation.numberinseries | 7 | |
dc.relation.volume | 10 | |
dc.type.version | publishedVersion | |
dc.rights.copyright | ©Authors, 2020 | |
dc.rights.accesslevel | openAccess | fi |
dc.subject.yso | enterovirukset | |
dc.subject.yso | spektroskopia | |
dc.subject.yso | fluoresenssi | |
dc.subject.yso | tutkimusmenetelmät | |
dc.subject.yso | infektiot | |
dc.subject.yso | RNA | |
dc.format.content | fulltext | |
jyx.subject.uri | http://www.yso.fi/onto/yso/p20689 | |
jyx.subject.uri | http://www.yso.fi/onto/yso/p10176 | |
jyx.subject.uri | http://www.yso.fi/onto/yso/p3265 | |
jyx.subject.uri | http://www.yso.fi/onto/yso/p415 | |
jyx.subject.uri | http://www.yso.fi/onto/yso/p7316 | |
jyx.subject.uri | http://www.yso.fi/onto/yso/p7689 | |
dc.rights.url | https://creativecommons.org/licenses/by/4.0/ | |
dc.relation.doi | 10.21769/BioProtoc.3582 | |
jyx.fundinginformation | We acknowledge Jane and Aatos Erkko foundation and doctoral school of University of Jyväskylä for funding and the authors of the original paper (Ruokolainen et al., 2019) for contributing to the work where the protocol was introduced. | |
dc.type.okm | A1 | |