Early entry events in Echovirus 30 infection
Vandesande, H., Laajala, M., Kantoluoto, T., Ruokolainen, V., Lindberg, A. M., & Marjomäki, V. (2020). Early entry events in Echovirus 30 infection. Journal of Virology, 94(13), Article e00592-20. https://doi.org/10.1128/JVI.00592-20
Published inJournal of Virology
© 2020 American Society for Microbiology.
Echovirus 30 (E30), a member of the enterovirus B species, is a major cause of viral meningitis, targeting children and adults alike. While it is a frequently isolated enterovirus and the cause of several outbreaks all over the world, suprisingly little is known regarding its entry and replication strategy within cells. In this study, we used E30 Bastianni (E30B) generated from an infectious cDNA clone in order to study early entry events during infection in human RD cells. E30B required the newly discovered Fc echovirus receptor (FcRn) for succesful infection, but not the Coxsackievirus and Adenovirus Receptor (CAR) or Decay-Accelerating Factor (DAF), although an interaction with DAF was observed. Double-stranded RNA replication intermediate was generated between 2 and 3 h post-infection (p.i.). and viral capsid production was initiated between 4 and 5 h p.i. The drugs affecting Rac1 (NSC 23766) and cholesterol (Filipin III) compromised infection, whereas bafilomycin A1, dyngo, U-73122, wortmannin and nocodazole did not, suggesting the virus follows an enterovirus-triggered macropinocytic pathway rather than the clathrin pathway. Colocalization with early endosomes and increased infection due to constitutively active Rab5 expression suggests some overlap and entry to classical early endosomes. Taken together, these results suggest that E30B induces an enterovirus entry pathway, leading to uncoating in early endosomes. ...
PublisherAmerican Society for Microbiology
Publication in research information system
MetadataShow full item record
Additional information about fundingNo funding information.
Showing items with similar title or keywords.
Karjalainen, Mikko (University of Jyväskylä, 2011)
Kakkonen, Elina (University of Jyväskylä, 2009)Elina Kakkonen tutki väitöskirjassaan kahden erilaisen viruksen, echovirus 1:n ja bakuloviruksen soluun sisäänmenon reittejä ja säätelijöitä. Echovirus 1 (EV1) on ihmisiä infektoiva pikornavirus, joka voi aiheuttaa esimerkiksi ...
Kangasniemi, Niko (2019)Enterovirukset ovat viruspeitteettömien, positiivisten RNA-virusten ryhmään kuuluva virusperhe, jonka jäsenet aiheuttamat mitä monituisimpia tauteja suu- ja sorkkataudista aina aivokalvontulehdukseen. Aikaisemmat ...
Martikainen, Mari (University of Jyväskylä, 2016)Enterovirus B group (EV-B) viruses are important human pathogens which cause a variety of diseases from mild respiratory illnesses to more severe acute infections such as myocarditis and meningitis. EV-Bs have also ...
Polyphenols Epigallocatechin Gallate and Resveratrol, and Polyphenol-Functionalized Nanoparticles Prevent Enterovirus Infection through Clustering and Stabilization of the Viruses Reshamwala, Dhanik; Shroff, Sailee; Sheik, Amamuddy Olivier; Laquintana, Valentino; Denora, Nunzio; Zacheo, Antonella; Lampinen, Vili; Hytonen, Vesa P.; Tastan, Bishop Özlem; Krol, Silke; Marjomäki, Varpu (MDPI AG, 2021)To efficiently lower virus infectivity and combat virus epidemics or pandemics, it is important to discover broadly acting antivirals. Here, we investigated two naturally occurring polyphenols, Epigallocatechin gallate ...