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dc.contributor.advisorVihinen-Ranta, Maija
dc.contributor.advisorAho, Vesa
dc.contributor.advisorMattola, Salla
dc.contributor.authorSalminen, Sami
dc.date.accessioned2020-03-13T10:47:56Z
dc.date.available2020-03-13T10:47:56Z
dc.date.issued2019
dc.identifier.urihttps://jyx.jyu.fi/handle/123456789/68153
dc.description.abstractHerpes simplex virus 1 (HSV-1) is a human pathogenic dsDNA virus capable of lytic orolabial infections in humans. Initial lytic infections lead to life-long and sporadically reactivating latent infections. In a lytic infection, viral DNA replication and progeny capsid assembly take place inside the host cell nucleus. The lytic infection results in extensive reorganization of nuclear structures and processes, including formation of viral replication compartments (VRCs) and marginalization of host chromatin to the nuclear periphery. Currently it is not known if these changes in nuclear architecture affect nucleocytoplasmic transport and nuclear permeability. Preliminary studies had indicated cytoplasmic histone accumulation during the infection, which could be due to changes in nuclear permeability. To study nucleocytoplasmic shuttling and localization of histones, confocal microscopy was used to analyze changes in histone distribution. Immunolabeling revealed an increase in the cytoplasmic localization of euchromatin (H3K27ac) marker and especially heterochromatin marker (H3K9me3) at 8 h and 12 h post infection (hpi). Fluorescence recovery after photobleaching (FRAP) experiments revealed a significant increase in the nuclear permeability of GFP at 12 hpi. Additionally, distribution of fluorescent importin β-EGFP shifted towards the nucleoplasm in infected cells at 8 and 12 hpi. These results suggest that marginalized chromatin does not hinder the nucleocytoplasmic shuttling of molecules and cytoplasmic accumulation of histones is not due to obstructed nuclear import. Instead, extranuclear histones and increase in nuclear permeability could be due to previously reported infection-induced nuclear pore impairment.en
dc.format.extent36
dc.format.mimetypeapplication/pdf
dc.language.isoen
dc.subject.othernuclear permeability
dc.subject.otherhistone distribution
dc.titleChanges in nuclear permeability and histone distribution during herpesvirus infection
dc.identifier.urnURN:NBN:fi:jyu-202003132400
dc.type.ontasotPro gradu -tutkielmafi
dc.type.ontasotMaster’s thesisen
dc.contributor.tiedekuntaMatemaattis-luonnontieteellinen tiedekuntafi
dc.contributor.tiedekuntaFaculty of Sciencesen
dc.contributor.laitosBio- ja ympäristötieteiden laitosfi
dc.contributor.laitosDepartment of Biological and Environmental Scienceen
dc.contributor.yliopistoJyväskylän yliopistofi
dc.contributor.yliopistoUniversity of Jyväskyläen
dc.contributor.oppiaineSolu- ja molekyylibiologiafi
dc.contributor.oppiaineCell and molecular biologyen
dc.rights.copyrightJulkaisu on tekijänoikeussäännösten alainen. Teosta voi lukea ja tulostaa henkilökohtaista käyttöä varten. Käyttö kaupallisiin tarkoituksiin on kielletty.fi
dc.rights.copyrightThis publication is copyrighted. You may download, display and print it for Your own personal use. Commercial use is prohibited.en
dc.type.publicationmasterThesis
dc.contributor.oppiainekoodi4013
dc.subject.ysosolubiologia
dc.subject.ysovirukset
dc.subject.ysoherpes simplex -virus
dc.subject.ysoherpesvirukset
dc.subject.ysoinfektiot
dc.subject.ysocell biology
dc.subject.ysoviruses
dc.subject.ysoherpes simplex virus
dc.subject.ysoherpesviruses
dc.subject.ysoinfections
dc.format.contentfulltext
dc.rights.accessrightsTekijä ei ole antanut lupaa avoimeen julkaisuun, joten aineisto on luettavissa vain Jyväskylän yliopiston kirjaston arkistotyösemalta. Ks. https://kirjasto.jyu.fi/fi/tyoskentelytilat/laitteet-ja-tilat..fi
dc.rights.accessrightsThe author has not given permission to make the work publicly available electronically. Therefore the material can be read only at the archival workstation at Jyväskylä University Library (https://kirjasto.jyu.fi/en/workspaces/facilities).en
dc.type.okmG2


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