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dc.contributor.authorVinner, Gurinder K.
dc.contributor.authorRichards, Kerry
dc.contributor.authorLeppänen, Miika
dc.contributor.authorSagona, Antonia P.
dc.contributor.authorMalik 1, Danish J.
dc.date.accessioned2019-09-30T08:52:54Z
dc.date.available2019-09-30T08:52:54Z
dc.date.issued2019
dc.identifier.citationVinner, G. K., Richards, K., Leppänen, M., Sagona, A. P., & Malik 1, D. J. (2019). Microencapsulation of Enteric Bacteriophages in a pH-Responsive Solid Oral Dosage Formulation Using a Scalable Membrane Emulsification Process. <i>Pharmaceutics</i>, <i>11</i>(9), Article 475. <a href="https://doi.org/10.3390/pharmaceutics11090475" target="_blank">https://doi.org/10.3390/pharmaceutics11090475</a>
dc.identifier.otherCONVID_33008034
dc.identifier.urihttps://jyx.jyu.fi/handle/123456789/65677
dc.description.abstractA scalable low-shear membrane emulsification process was used to produce microencapsulated Escherichia coli-phages in a solid oral dosage form. Uniform pH-responsive composite microparticles (mean size ~100 µm) composed of Eudragit® S100 and alginate were produced. The internal microstructure of the gelled microcapsules was studied using ion-milling and imaging, which showed that the microparticles had a solid internal core. The microencapsulation process significantly protected phages upon prolonged exposure to a simulated gastric acidic environment. Encapsulated phages that had been pre-exposed to simulated gastric acid were added to actively growing bacterial cells using in vitro cell cultures and were found to be effective in killing E. coli. Encapsulated phages were also shown to be effective in killing actively growing E. coli in the presence of human epithelial cells. Confocal microscopy images showed that the morphology of encapsulated phage-treated epithelial cells was considerably better than controls without phage treatment. The encapsulated phages were stable during refrigerated storage over a four-week period. The process of membrane emulsification is highly scalable and is a promising route to produce industrial quantities of pH-responsive oral solid dosage forms suitable for delivering high titres of viable phages to the gastrointestinal tract.en
dc.format.mimetypeapplication/pdf
dc.languageeng
dc.language.isoeng
dc.publisherMDPI
dc.relation.ispartofseriesPharmaceutics
dc.rightsCC BY 4.0
dc.subject.othermicroencapsulation
dc.subject.otherbacteriophage therapy
dc.subject.othercontrolled release
dc.subject.otherenteric infections
dc.subject.otherpH-triggered release
dc.subject.otherE. coli
dc.subject.otherEudragit S100
dc.titleMicroencapsulation of Enteric Bacteriophages in a pH-Responsive Solid Oral Dosage Formulation Using a Scalable Membrane Emulsification Process
dc.typearticle
dc.identifier.urnURN:NBN:fi:jyu-201909304283
dc.contributor.laitosFysiikan laitosfi
dc.contributor.laitosDepartment of Physicsen
dc.type.urihttp://purl.org/eprint/type/JournalArticle
dc.description.reviewstatuspeerReviewed
dc.relation.issn1999-4923
dc.relation.numberinseries9
dc.relation.volume11
dc.type.versionpublishedVersion
dc.rights.copyright© 2019 by the authors
dc.rights.accesslevelopenAccessfi
dc.subject.ysomikrorakenteet
dc.subject.ysobakteriofagit
dc.subject.ysokalvot (tekniikka)
dc.subject.ysomaha-suolitulehdus
dc.subject.ysoenterobakteerit
dc.subject.ysofagiterapia
dc.subject.ysolääkesuunnittelu
dc.format.contentfulltext
jyx.subject.urihttp://www.yso.fi/onto/yso/p24463
jyx.subject.urihttp://www.yso.fi/onto/yso/p25303
jyx.subject.urihttp://www.yso.fi/onto/yso/p22892
jyx.subject.urihttp://www.yso.fi/onto/yso/p22919
jyx.subject.urihttp://www.yso.fi/onto/yso/p28554
jyx.subject.urihttp://www.yso.fi/onto/yso/p29496
jyx.subject.urihttp://www.yso.fi/onto/yso/p25180
dc.rights.urlhttps://creativecommons.org/licenses/by/4.0/
dc.relation.doi10.3390/pharmaceutics11090475


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