Visualizing a protein quake with time-resolved X-ray scattering at a free-electron laser
Arnlund, D., Johansson, L. C., Wickstrand, C., Barty, A., Williams, G. J., Malmerberg, E., Davidsson, J., Milathianaki, D., DePonte, D. P., Shoeman, R. L., Wang, D., James, D., Katona, G., Westenhoff, S., White, T. A., Aquila, A., Bari, S., Berntsen, P., Bogan, M., . . . Neutze, R. (2014). Visualizing a protein quake with time-resolved X-ray scattering at a free-electron laser. Nature methods, 11(9), 923-926. https://doi.org/10.1038/nmeth.3067
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Nature methodsAuthors
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2014Discipline
Epäorgaaninen ja analyyttinen kemiaNanoscience CenterInorganic and Analytical ChemistryNanoscience CenterCopyright
© 2014 Springer Nature
We describe a method to measure ultrafast protein structural changes using time-resolved wide-angle X-ray scattering at an X-ray free-electron laser. We demonstrated this approach using multiphoton excitation of the Blastochloris viridis photosynthetic reaction center, observing an ultrafast global conformational change that arises within picoseconds and precedes the propagation of heat through the protein. This provides direct structural evidence for a 'protein quake': the hypothesis that proteins rapidly dissipate energy through quake-like structural motions.
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Nature Publishing GroupISSN Search the Publication Forum
1548-7091Publication in research information system
https://converis.jyu.fi/converis/portal/detail/Publication/23875342
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