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dc.contributor.authorBerntsson, Oskar
dc.contributor.authorRodriguez, Ryan
dc.contributor.authorHenry, Léocadie
dc.contributor.authorPanman, Matthijs R.
dc.contributor.authorHughes, Ashley J.
dc.contributor.authorEinholz, Christopher
dc.contributor.authorWeber, Stefan
dc.contributor.authorIhalainen, Janne A.
dc.contributor.authorHenning, Robert
dc.contributor.authorKosheleva, Irina
dc.contributor.authorSchleicher, Erik
dc.contributor.authorWestenhoff, Sebastian
dc.date.accessioned2019-07-24T11:07:36Z
dc.date.available2019-07-24T11:07:36Z
dc.date.issued2019
dc.identifier.citationBerntsson, Oskar; Rodriguez, Ryan; Henry, Léocadie; Panman, Matthijs R.; Hughes, Ashley J.; Einholz, Christopher; Weber, Stefan; Ihalainen, Janne A.; Henning, Robert; Kosheleva, Irina et al. (2019). Photoactivation of Drosophila melanogaster cryptochrome through sequential conformational transitions. Science Advances, 5 (7), eaaw1531. DOI: 10.1126/sciadv.aaw1531
dc.identifier.otherCONVID_32141858
dc.identifier.urihttps://jyx.jyu.fi/handle/123456789/65105
dc.description.abstractCryptochromes are blue-light photoreceptor proteins, which provide input to circadian clocks. The crypto- chrome from Drosophila melanogaster (DmCry) modulates the degradation of Timeless and itself. It is unclear how light absorption by the chromophore and the subsequent redox reactions trigger these events. Here, we use nano- to millisecond time-resolved x-ray solution scattering to reveal the light-activated conformational changes in DmCry and the related (6-4) photolyase. DmCry undergoes a series of structural changes, culminat- ing in the release of the carboxyl-terminal tail (CTT). The photolyase has a simpler structural response. We find that the CTT release in DmCry depends on pH. Mutation of a conserved histidine, important for the biochemical activity of DmCry, does not affect transduction of the structural signal to the CTT. Instead, molecular dynamics simulations suggest that it stabilizes the CTT in the resting-state conformation. Our structural photocycle un- ravels the first molecular events of signal transduction in an animal cryptochrome.en
dc.format.mimetypeapplication/pdf
dc.languageeng
dc.publisherAmerican Association for the Advancement of Science
dc.relation.ispartofseriesScience Advances
dc.rightsCC BY-NC 4.0
dc.subject.otherDrosophila melanogaster
dc.titlePhotoactivation of Drosophila melanogaster cryptochrome through sequential conformational transitions
dc.typearticle
dc.identifier.urnURN:NBN:fi:jyu-201907243668
dc.contributor.laitosDepartment of Biological and Environmental Scienceen
dc.contributor.laitosBio- ja ympäristötieteiden laitosfi
dc.type.urihttp://purl.org/eprint/type/JournalArticle
dc.description.reviewstatuspeerReviewed
dc.relation.issn2375-2548
dc.relation.numberinseries7
dc.relation.volume5
dc.type.versionpublishedVersion
dc.rights.copyright© 2019 The Authors
dc.rights.accesslevelopenAccessfi
dc.relation.grantnumber296135
dc.subject.ysovuorokausirytmi
dc.subject.ysobanaanikärpänen
dc.subject.ysoproteiinit
dc.subject.ysofotobiologia
dc.format.contentfulltext
jyx.subject.urihttp://www.yso.fi/onto/yso/p16613
jyx.subject.urihttp://www.yso.fi/onto/yso/p24873
jyx.subject.urihttp://www.yso.fi/onto/yso/p4332
jyx.subject.urihttp://www.yso.fi/onto/yso/p27666
dc.rights.urlhttps://creativecommons.org/licenses/by-nc/4.0/
dc.relation.doi10.1126/sciadv.aaw1531
dc.relation.funderAcademy of Finland
jyx.fundingprogramAcademy Project, AoF
jyx.fundinginformationS.Wes. acknowledges funding from the Swedish Foundation for International Cooperation in Research and Higher Education, the European Research Council (725642), and the Foundation of Strategic Research, Sweden. E.S. and S.Web. thank the DFG (235777276/GRK1976) for financial support. J.A.I. acknowledges a grant from the Academy of Finland (296135) and the Jane and Aatos Erkko Foundation.


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