dc.contributor.author | Vuornos, Kaisa | |
dc.contributor.author | Ojansivu, Miina | |
dc.contributor.author | Koivisto, Janne T. | |
dc.contributor.author | Häkkänen, Heikki | |
dc.contributor.author | Belay, Birhanu | |
dc.contributor.author | Montonen, Toni | |
dc.contributor.author | Huhtala, Heini | |
dc.contributor.author | Kääriäinen, Minna | |
dc.contributor.author | Hupa, Leena | |
dc.contributor.author | Kellomäki, Minna | |
dc.contributor.author | Hyttinen, Jari | |
dc.contributor.author | Ihalainen, Janne | |
dc.contributor.author | Miettinen, Susanna | |
dc.date.accessioned | 2019-03-19T09:33:54Z | |
dc.date.available | 2021-06-01T21:35:08Z | |
dc.date.issued | 2019 | |
dc.identifier.citation | Vuornos, K., Ojansivu, M., Koivisto, J. T., Häkkänen, H., Belay, B., Montonen, T., Huhtala, H., Kääriäinen, M., Hupa, L., Kellomäki, M., Hyttinen, J., Ihalainen, J., & Miettinen, S. (2019). Bioactive glass ions induce efficient osteogenic differentiation of human adipose stem cells encapsulated in gellan gum and collagen type I hydrogels. <i>Materials Science and Engineering C</i>, <i>99</i>, 905-918. <a href="https://doi.org/10.1016/j.msec.2019.02.035" target="_blank">https://doi.org/10.1016/j.msec.2019.02.035</a> | |
dc.identifier.other | CONVID_28928851 | |
dc.identifier.uri | https://jyx.jyu.fi/handle/123456789/63215 | |
dc.description.abstract | Background: Due to unmet need for bone augmentation, our aim was to promote osteogenic
differentiation of human adipose stem cells (hASCs) encapsulated in gellan gum (GG) or collagen
type I (COL) hydrogels with bioactive glass (experimental glass 2-06 of composition [wt-%]: Na2O
12.1, K2O 14.0, CaO 19.8, P2O5 2.5, B2O3 1.6, SiO2 50.0) extract based osteogenic medium (BaG
OM) for bone construct development. GG hydrogels were crosslinked with spermidine (GG-SPD)
or BaG extract (GG-BaG).
Methods: Mechanical properties of cell-free GG-SPD, GG-BaG, and COL hydrogels were tested in
osteogenic medium (OM) or BaG OM at 0, 14, and 21d. Hydrogel embedded hASCs were cultured
in OM or BaG OM for 3, 14, and 21d, and analyzed for viability, cell number, osteogenic gene
expression, osteocalcin production, and mineralization. Hydroxyapatite-stained GG-SPD samples
were imaged with Optical Projection Tomography (OPT) and Selective Plane Illumination
Microscopy (SPIM) in OM and BaG OM at 21d. Furthermore, Raman spectroscopy was used to
study the calcium phosphate (CaP) content of hASC-secreted ECM in GG-SPD, GG-BaG, and COL
at 21d in BaG OM.
Results: The results showed viable rounded cells in GG whereas hASCs were elongated in COL.
Importantly, BaG OM induced significantly higher cell number and higher osteogenic gene
expression in COL. In both hydrogels, BaG OM induced strong mineralization confirmed as CaP by
Raman spectroscopy and significantly improved mechanical properties. GG-BaG hydrogels rescued
hASC mineralization in OM. OPT and SPIM showed homogeneous 3D cell distribution with strong
mineralization in BaG OM. Also, strong osteocalcin production was visible in COL.
Conclusions: Overall, we showed efficacious osteogenesis of hASCs in 3D hydrogels with BaG
OM with potential for bone-like grafts. | fi |
dc.format.mimetype | application/pdf | |
dc.language.iso | eng | |
dc.publisher | Elsevier | |
dc.relation.ispartofseries | Materials Science and Engineering C | |
dc.rights | CC BY-NC-ND 4.0 | |
dc.subject.other | adipose stem cell | |
dc.subject.other | bioactive glass | |
dc.subject.other | osteogenic differentiation | |
dc.subject.other | gellan gum hydrogel | |
dc.subject.other | collagen type I hydrogel | |
dc.title | Bioactive glass ions induce efficient osteogenic differentiation of human adipose stem cells encapsulated in gellan gum and collagen type I hydrogels | |
dc.type | research article | |
dc.identifier.urn | URN:NBN:fi:jyu-201903081782 | |
dc.contributor.laitos | Bio- ja ympäristötieteiden laitos | fi |
dc.contributor.laitos | Department of Biological and Environmental Science | en |
dc.contributor.oppiaine | Solu- ja molekyylibiologia | fi |
dc.contributor.oppiaine | Nanoscience Center | fi |
dc.contributor.oppiaine | Cell and Molecular Biology | en |
dc.contributor.oppiaine | Nanoscience Center | en |
dc.type.uri | http://purl.org/eprint/type/JournalArticle | |
dc.date.updated | 2019-03-08T10:15:07Z | |
dc.type.coar | http://purl.org/coar/resource_type/c_2df8fbb1 | |
dc.description.reviewstatus | peerReviewed | |
dc.format.pagerange | 905-918 | |
dc.relation.issn | 0928-4931 | |
dc.relation.numberinseries | 0 | |
dc.relation.volume | 99 | |
dc.type.version | acceptedVersion | |
dc.rights.copyright | © 2019 Elsevier B.V. | |
dc.rights.accesslevel | openAccess | fi |
dc.type.publication | article | |
dc.subject.yso | luukudokset | |
dc.subject.yso | implantit | |
dc.subject.yso | lasi | |
dc.subject.yso | biologinen aktiivisuus | |
dc.subject.yso | kantasolut | |
dc.subject.yso | geelit | |
dc.format.content | fulltext | |
jyx.subject.uri | http://www.yso.fi/onto/yso/p24381 | |
jyx.subject.uri | http://www.yso.fi/onto/yso/p20281 | |
jyx.subject.uri | http://www.yso.fi/onto/yso/p16484 | |
jyx.subject.uri | http://www.yso.fi/onto/yso/p24582 | |
jyx.subject.uri | http://www.yso.fi/onto/yso/p13517 | |
jyx.subject.uri | http://www.yso.fi/onto/yso/p12973 | |
dc.rights.url | https://creativecommons.org/licenses/by-nc-nd/4.0/ | |
dc.relation.doi | 10.1016/j.msec.2019.02.035 | |
dc.type.okm | A1 | |