Alternative biosynthetic starter units enhance the structural diversity of cyanobacterial lipopeptides
Mareš, J., Hájek, J., Urajová, P., Kust, A., Jokela, J., Saurav, K., Galica, T., Čapková, K., Mattila, A., Haapaniemi, E., Permi, P., Mysterud, I., Skulberg, O. M., Karlsen, J., Fewer, D. P., Sivonen, K., Tønnesen, H. H., & Hrouzek, P. (2019). Alternative biosynthetic starter units enhance the structural diversity of cyanobacterial lipopeptides. Applied and Environmental Microbiology, 85(4), Article e02675-18. https://doi.org/10.1128/AEM.02675-18
Published inApplied and Environmental Microbiology
DisciplineSolu- ja molekyylibiologiaOrgaaninen kemiaNanoscience CenterCell and Molecular BiologyOrganic ChemistryNanoscience Center
© 2019 American Society for Microbiology
Puwainaphycins (PUWs) and minutissamides (MINs) are structurally analogous cyclic lipopeptides possessing cytotoxic activity. Both types of compound exhibit high structural variability, particularly in the fatty acid (FA) moiety. Although a biosynthetic gene cluster responsible for synthesis of several PUW variants has been proposed in a cyanobacterial strain, the genetic background for MINs remains unexplored. Herein, we report PUW/MIN biosynthetic gene clusters and structural variants from six cyanobacterial strains. Comparison of biosynthetic gene clusters indicates a common origin of the PUW/MIN hybrid nonribosomal peptide synthetase and polyketide synthase. Surprisingly, the biosynthetic gene clusters encode two alternative biosynthetic starter modules, and analysis of structural variants suggests that initiation by each of the starter modules results in lipopeptides of differing lengths and FA substitutions. Among additional modifications of the FA chain, chlorination of minutissamide D was explained by the presence of a putative halogenase gene in the PUW/MIN gene cluster of Anabaena minutissima strain UTEX B 1613. We detected PUW variants bearing an acetyl substitution in Symplocastrum muelleri strain NIVA-CYA 644, consistent with an O-acetyltransferase gene in its biosynthetic gene cluster. The major lipopeptide variants did not exhibit any significant antibacterial activity, and only the PUW F variant was moderately active against yeast, consistent with previously published data suggesting that PUWs/MINs interact preferentially with eukaryotic plasma membranes. IMPORTANCE Herein, we deciphered the most important biosynthetic traits of a prominent group of bioactive lipopeptides. We reveal evidence for initiation of biosynthesis by two alternative starter units hardwired directly in the same gene cluster, eventually resulting in the production of a remarkable range of lipopeptide variants. We identified several unusual tailoring genes potentially involved in modifying the fatty acid chain. Careful characterization of these biosynthetic gene clusters and their diverse products could provide important insight into lipopeptide biosynthesis in prokaryotes. Some of the variants identified exhibit cytotoxic and antifungal properties, and some are associated with a toxigenic biofilm-forming strain. The findings may prove valuable to researchers in the fields of natural product discovery and toxicology. ...
PublisherAmerican Society for Microbiology
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