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dc.contributor.authorShao, Shuai
dc.contributor.authorLiao, Xiaoling
dc.contributor.authorXie, Fei
dc.contributor.authorDeng, Sha
dc.contributor.authorLiu, Xue
dc.contributor.authorRistaniemi, Tapani
dc.contributor.authorLiu, Bo
dc.date.accessioned2019-01-03T12:38:51Z
dc.date.available2019-01-03T12:38:51Z
dc.date.issued2018fi
dc.identifier.citationShao, S., Liao, X., Xie, F., Deng, S., Liu, X., Ristaniemi, T., & Liu, B. (2018). FRET biosensor allows spatio-temporal observation of shear stress-induced polar RhoGDIα activation. <em>Communications Biology</em>, 1, 224. <a href="https://doi.org/10.1038/s42003-018-0232-2">doi:10.1038/s42003-018-0232-2</a>fi
dc.identifier.otherTUTKAID_79821
dc.identifier.urihttps://jyx.jyu.fi/handle/123456789/60867
dc.description.abstractRho GDP-dissociation inhibitor α (RhoGDIα) is a known negative regulator of the Rho family that shuts off GDP/GTP cycling and cytoplasm/membrane translocation to regulate cell migration. However, to our knowledge, no reports are available that focus on how the RhoGDIα-Rho GTPases complex is activated by laminar flow through exploring the activation of RhoGDIα itself. Here, we constructed a new biosensor using fluorescence resonance energy transfer (FRET) technology to measure the spatio-temporal activation of RhoGDIα in its binding with Rho GTPases in living HeLa cells. Using this biosensor, we find that the dissociation of the RhoGDIα-Rho GTPases complex is increased by shear stress, and its dissociation rate varies with subcellular location. Moreover, this process is mediated by membrane fluidity, cytoskeleton and Src activity, which indicates that the regulation of RhoGDIα activation under shear stress application represents a relatively separate pathway from the shear stress-induced Rho pathway.fi
dc.format.mimetypeapplication/pdf
dc.language.isoeng
dc.publisherNature Publishing Group
dc.relation.ispartofseriesCommunications Biology
dc.rightsCC BY 4.0
dc.subject.otherbiosensoritfi
dc.subject.otherbiosensorsfi
dc.titleFRET biosensor allows spatio-temporal observation of shear stress-induced polar RhoGDIα activationfi
dc.typearticle
dc.identifier.urnURN:NBN:fi:jyu-201812125074
dc.contributor.laitosInformaatioteknologian tiedekuntafi
dc.contributor.laitosFaculty of Information Technologyen
dc.contributor.oppiaineTietotekniikka
dc.type.urihttp://purl.org/eprint/type/JournalArticle
dc.date.updated2018-12-12T07:15:16Z
dc.description.reviewstatuspeerReviewed
dc.relation.issn2399-3642
dc.relation.numberinseries0
dc.relation.volume1
dc.type.versionpublishedVersion
dc.rights.copyright© The Author(s) 2018
dc.rights.accesslevelopenAccessfi
dc.format.contentfulltext
dc.rights.urlhttps://creativecommons.org/licenses/by/4.0/
dc.relation.doi10.1038/s42003-018-0232-2


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