Broad thermal tolerance is negatively correlated with virulence in an opportunistic bacterial pathogen
Ashrafi, R., Bruneaux, M., Sundberg, L.-R., Pulkkinen, K., Valkonen, J., & Ketola, T. (2018). Broad thermal tolerance is negatively correlated with virulence in an opportunistic bacterial pathogen. Evolutionary Applications, 11(9), 1700-1714. https://doi.org/10.1111/eva.12673
Published inEvolutionary Applications
DisciplineEkologia ja evoluutiobiologiaSolu- ja molekyylibiologiaAkvaattiset tieteetEcology and Evolutionary BiologyCell and Molecular BiologyAquatic Sciences
© 2018 The Authors
Predicting the effects of global increase in temperatures on disease virulence is challenging, especially for environmental opportunistic bacteria, because pathogen fitness may be differentially affected by temperature within and outside host environment. So far, there is very little empirical evidence on the connections between optimal temperature range and virulence in environmentally growing pathogens. Here, we explored whether the virulence of an environmentally growing opportunistic fish pathogen, Flavobacterium columnare, is malleable to evolutionary changes via correlated selection on thermal tolerance. To this end, we experimentally quantified the thermal performance curves (TPCs) for maximum biomass yield of 49 F. columnare isolates from eight different geographic locations in Finland over ten years (2003–2012). We also characterized virulence profiles of these strains in a zebra fish (Danio rerio) infection model. We show that virulence among the strains increased over the years, but thermal generalism, and in particular tolerance to higher temperatures, was negatively associated with virulence. Our data suggest that temperature has a strong effect on the pathogen genetic diversity and therefore presumably also on disease dynamics. However, the observed increase in frequency and severity of F. columnare epidemics over the last decade cannot be directly linked to bacterial evolution due to increased mean temperature, but is most likely associated with factors related to increased length of growing season, or other time‐dependent change in environment. Our study demonstrates that complex interactions between the host, the pathogen and the environment influence disease virulence of an environmentally growing opportunistic pathogen. ...
PublisherWiley-Blackwell Publishing, Inc.
Dataset related to the publicationhttps://doi.org/10.5061/dryad.858j5m7
Publication in research information system
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Related funder(s)Academy of Finland
Funding program(s)Research post as Academy Research Fellow, AoF; Others, AoF
Additional information about fundingThis work was supported by KONE Foundation (Roghaieh Ashrafi via project “Constraints of evolutionary adaptation to climate change” to Tarmo Ketola), OLVI Foundation (Roghaieh Ashrafi #201620393), the Jane and Aatos Erkko Foundation (Lotta‐Riina Sundberg), Finnish Cultural Foundation (Katja Pulkkinen) and Academy of Finland (Lotta‐Riina Sundberg # 266879 and # 304615, Tarmo Ketola # 278751, Jouni Taskinen # 260704 to Katja Pulkkinen) and Centre of Excellence in Biological Interactions (# 252411, Prof. Johanna Mappes) to Roghaieh Ashrafi, Lotta‐Riina Sundberg and Tarmo Ketola. ...
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