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dc.contributor.authorMunukka, Eveliina
dc.contributor.authorAhtiainen, Juha
dc.contributor.authorPuigbo, Pere
dc.contributor.authorJalkanen, Sirpa
dc.contributor.authorPahkala, Katja
dc.contributor.authorRintala, Anniina
dc.contributor.authorKujala, Urho
dc.contributor.authorPietilä, Sami
dc.contributor.authorHollmén, Maija
dc.contributor.authorElo, Laura
dc.contributor.authorHuovinen, Pentti
dc.contributor.authorD'Auria, Giuseppe
dc.contributor.authorPekkala, Satu
dc.date.accessioned2018-10-25T07:47:45Z
dc.date.available2018-10-25T07:47:45Z
dc.date.issued2018
dc.identifier.citationMunukka, E., Ahtiainen, J., Puigbo, P., Jalkanen, S., Pahkala, K., Rintala, A., Kujala, U., Pietilä, S., Hollmén, M., Elo, L., Huovinen, P., D'Auria, G., & Pekkala, S. (2018). Six-Week Endurance Exercise Alters Gut Metagenome That Is not Reflected in Systemic Metabolism in Over-weight Women. <i>Frontiers in Microbiology</i>, <i>9</i>, Article 2323. <a href="https://doi.org/10.3389/fmicb.2018.02323" target="_blank">https://doi.org/10.3389/fmicb.2018.02323</a>
dc.identifier.otherCONVID_28271123
dc.identifier.otherTUTKAID_78885
dc.identifier.urihttps://jyx.jyu.fi/handle/123456789/59950
dc.description.abstractRecent studies suggest that exercise alters the gut microbiome. We determined whether six-weeks endurance exercise, without changing diet, affected the gut metagenome and systemic metabolites of overweight women. Previously sedentary overweight women (n = 19) underwent a six-weeks endurance exercise intervention, but two were excluded due to antibiotic therapy. The gut microbiota composition and functions were analyzed by 16S rRNA gene amplicon sequencing and metagenomics. Body composition was analyzed with DXA X-ray densitometer and serum metabolomics with NMR metabolomics. Total energy and energy-yielding nutrient intakes were analyzed from food records using Micro-Nutrica software. Serum clinical variables were determined with KONELAB instrument. Soluble Vascular Adhesion Protein 1 (VAP-1) was measured with ELISA and its' enzymatic activity as produced hydrogen peroxide. The exercise intervention was effective, as maximal power and maximum rate of oxygen consumption increased while android fat mass decreased. No changes in diet were observed. Metagenomic analysis revealed taxonomic shifts including an increase in Akkermansia and a decrease in Proteobacteria. These changes were independent of age, weight, fat % as well as energy and fiber intake. Training slightly increased Jaccard distance of genus level β-diversity. Training did not alter the enriched metagenomic pathways, which, according to Bray Curtis dissimilarity analysis, may have been due to that only half of the subjects' microbiomes responded considerably to exercise. Nevertheless, tranining decreased the abundance of several genes including those related to fructose and amino acid metabolism. These metagenomic changes, however, were not translated into major systemic metabolic changes as only two metabolites, phospholipids and cholesterol in large VLDL particles, decreased after exercise. Training also decreased the amine oxidase activity of pro-inflammatory VAP-1, whereas no changes in CRP were detected. All clinical blood variables were within normal range, yet exercise slightly increased glucose and decreased LDL and HDL. In conclusion, exercise training modified the gut microbiome without greatly affecting systemic metabolites or body composition. Based on our data and existing literature, we propose that especially Akkermansia and Proteobacteria are exercise-responsive taxa. Our results warrant the need for further studies in larger cohorts to determine whether exercise types other than endurance exercise also modify the gut metagenome.fi
dc.format.mimetypeapplication/pdf
dc.language.isoeng
dc.publisherFrontiers Research Foundation
dc.relation.ispartofseriesFrontiers in Microbiology
dc.rightsCC BY 4.0
dc.subject.otherexercise intervention
dc.subject.othergut microbiota composition
dc.subject.othergut microbiota function
dc.subject.othersystemic metabolites
dc.subject.othercardiovascular effects
dc.titleSix-Week Endurance Exercise Alters Gut Metagenome That Is not Reflected in Systemic Metabolism in Over-weight Women
dc.typearticle
dc.identifier.urnURN:NBN:fi:jyu-201810044347
dc.contributor.laitosLiikuntatieteellinen tiedekuntafi
dc.contributor.laitosFaculty of Sport and Health Sciencesen
dc.contributor.oppiaineLiikuntalääketiedefi
dc.contributor.oppiaineValmennus- ja testausoppifi
dc.contributor.oppiaineSports and Exercise Medicineen
dc.contributor.oppiaineScience of Sport Coaching and Fitness Testingen
dc.type.urihttp://purl.org/eprint/type/JournalArticle
dc.date.updated2018-10-04T09:15:24Z
dc.description.reviewstatuspeerReviewed
dc.relation.issn1664-302X
dc.relation.numberinseries0
dc.relation.volume9
dc.type.versionpublishedVersion
dc.rights.copyright© 2018 Munukka, Ahtiainen, Puigbó, Jalkanen, Pahkala, Keskitalo, Kujala, Pietilä, Hollmén, Elo, Huovinen, D'Auria and Pekkala.
dc.rights.accesslevelopenAccessfi
dc.subject.ysokestävyysharjoittelu
dc.subject.ysosuolistomikrobisto
dc.subject.ysoaineenvaihdunta
dc.subject.ysoylipaino
dc.subject.ysonaiset
dc.format.contentfulltext
jyx.subject.urihttp://www.yso.fi/onto/yso/p7676
jyx.subject.urihttp://www.yso.fi/onto/yso/p37925
jyx.subject.urihttp://www.yso.fi/onto/yso/p3066
jyx.subject.urihttp://www.yso.fi/onto/yso/p826
jyx.subject.urihttp://www.yso.fi/onto/yso/p16991
dc.rights.urlhttps://creativecommons.org/licenses/by/4.0/
dc.relation.doi10.3389/fmicb.2018.02323


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