Six-Week Endurance Exercise Alters Gut Metagenome That Is not Reflected in Systemic Metabolism in Over-weight Women
Munukka, E., Ahtiainen, J., Puigbo, P., Jalkanen, S., Pahkala, K., Rintala, A., Kujala, U., Pietilä, S., Hollmén, M., Elo, L., Huovinen, P., D'Auria, G., & Pekkala, S. (2018). Six-Week Endurance Exercise Alters Gut Metagenome That Is not Reflected in Systemic Metabolism in Over-weight Women. Frontiers in Microbiology, 9, Article 2323. https://doi.org/10.3389/fmicb.2018.02323
Published inFrontiers in Microbiology
DisciplineLiikuntalääketiedeValmennus- ja testausoppiSports and Exercise MedicineScience of Sport Coaching and Fitness Testing
© 2018 Munukka, Ahtiainen, Puigbó, Jalkanen, Pahkala, Keskitalo, Kujala, Pietilä, Hollmén, Elo, Huovinen, D'Auria and Pekkala.
Recent studies suggest that exercise alters the gut microbiome. We determined whether six-weeks endurance exercise, without changing diet, affected the gut metagenome and systemic metabolites of overweight women. Previously sedentary overweight women (n = 19) underwent a six-weeks endurance exercise intervention, but two were excluded due to antibiotic therapy. The gut microbiota composition and functions were analyzed by 16S rRNA gene amplicon sequencing and metagenomics. Body composition was analyzed with DXA X-ray densitometer and serum metabolomics with NMR metabolomics. Total energy and energy-yielding nutrient intakes were analyzed from food records using Micro-Nutrica software. Serum clinical variables were determined with KONELAB instrument. Soluble Vascular Adhesion Protein 1 (VAP-1) was measured with ELISA and its' enzymatic activity as produced hydrogen peroxide. The exercise intervention was effective, as maximal power and maximum rate of oxygen consumption increased while android fat mass decreased. No changes in diet were observed. Metagenomic analysis revealed taxonomic shifts including an increase in Akkermansia and a decrease in Proteobacteria. These changes were independent of age, weight, fat % as well as energy and fiber intake. Training slightly increased Jaccard distance of genus level β-diversity. Training did not alter the enriched metagenomic pathways, which, according to Bray Curtis dissimilarity analysis, may have been due to that only half of the subjects' microbiomes responded considerably to exercise. Nevertheless, tranining decreased the abundance of several genes including those related to fructose and amino acid metabolism. These metagenomic changes, however, were not translated into major systemic metabolic changes as only two metabolites, phospholipids and cholesterol in large VLDL particles, decreased after exercise. Training also decreased the amine oxidase activity of pro-inflammatory VAP-1, whereas no changes in CRP were detected. All clinical blood variables were within normal range, yet exercise slightly increased glucose and decreased LDL and HDL. In conclusion, exercise training modified the gut microbiome without greatly affecting systemic metabolites or body composition. Based on our data and existing literature, we propose that especially Akkermansia and Proteobacteria are exercise-responsive taxa. Our results warrant the need for further studies in larger cohorts to determine whether exercise types other than endurance exercise also modify the gut metagenome. ...
PublisherFrontiers Research Foundation
Publication in research information system
MetadataShow full item record
- Liikuntatieteiden tiedekunta 
Showing items with similar title or keywords.
Lensu, Sanna; Pekkala, Satu (MDPI AG, 2021)Trillions of microbes inhabiting the gut modulate the metabolism of the host. Cross-sectional studies have reported associations between physical performance and the gut microbiota (GM). Physical activity seems to increase ...
Kauko, Kirsi (2010)Abstract Effect of Short-Term Guided Aerobic Exercise and Diet Intervention on Overweight Women Kauko, Kirsi LLTS007 Master’s Thesis of Sport Medicine University of Jyväskylä, Faculty of Sport and Health Sciences, Department ...
Effects of a weight management program delivered by social media on weight and metabolic syndrome risk factors in overweight and obese adults : A randomised controlled trial Jane, Monica; Hagger, Martin; Foster, Jonathan; Ho, Suleen; Kane, Robert; Pal, Sebely (Public Library of Science, 2017)Introduction The aim of this project was to evaluate the effectiveness of using social media to augment the delivery of, and provide support for, a weight management program delivered to overweight and obese individuals ...
Body composition and molecular reflections of obesity-related cardio-metabolic disorders : a cross-sectional and longitudinal study in women Wiklund, Petri (University of Jyväskylä, 2016)
Intrinsic aerobic capacity governs the associations between gut microbiota composition and fat metabolism age-dependently in rat siblings Pekkala, Satu; Lensu, Sanna; Nokia, Miriam; Vanhatalo, Sanja; Koch, Lauren G.; Britton, Steven L.; Kainulainen, Heikki (American Physiological Society, 2017)Host genetic factors affecting the gut microbiome play an important role in obesity, yet limited attention has been paid on the host genetic factors linked to physical fitness in modifying the microbiome. This study ...